Objective:To explore protective action of ultramicro-powder of Rhizoma Gastrodiae on nerves in the rat of Cerebral ischemia- reperfusion injury.Methods:The local ischemia-reperfusion injury at model was established by cerebral ischemia for 2 hours and reperfusion for 24 hours.HE staining,2,3,5-triphenytetrazolium(TTC)staining,immunohistochemical methods were respectively used to investigate pathological changes of brain slices,the size of cerebral infarction and nervous cell apoptotic relative proteins Bcl-2,Bax expression after treatment with ultramicro-powder of Rhizoma Gastrodiae.Results:(1)Ultramicro-powder of Rhizoma Gastrodiae could reduce the size of cerebral infarction.When the low,middle and high dose groups were compared with the ischemia group there were significant differences(P

Environmental Monitoring ; methods ; Noise, Occupational ; statistics & numerical data

Activated protein kinase ( AMPK) , an important energy receptor , plays a very important role in regulating organ-ism and the energy metabolism of the cell .AMPK has complex relationship with survival of different types of breast cancer cells .Ac-cording to different conditions , AMPK may exist both anti or promoting effect on tumor .In this review , we summarize the relationship between AMPK and breast cancer related signal pathways , AMP and breast cancer endocrine therapy , breast cancer chemotherapy , ra-diotherapy for breast cancer , treatment of triple negative breast cancer and multi drug resistance of the relationship , we also expound some drugs related to AMPK and used in clinical setting .

Objective In the LPS-induced acute lung injury (ALI) there is massive accumulation of neutrophils in the lung. The effect of neutrophil collagenase on the degradation of lung matrix collagen ig still unknown. The aim of this study was to investigate the role of neutrophil collagenase in LPS-induced ALI.Methods Thirty SD rats of either sex weighing 190 ? 40 g were randomly divided into five groups of 6 animals : group 1 control and group 2-5 LPS. In LPS groups animals were anesthetized with intraperitoneal 3% pentobarbital 30 mg?kg-1. Right jugular vein was cannulated. LPS (O55: B5 Sigma USA) 5 mg?kg-1 was injected iv. The animals were sacrificed at 2 h (group 2), 4 h (group 3), 6 h( group 4) and 8 h( group 5) after iv LPS administration. Bronchoalveolar lavage was performed immediately with 3 ml of normal saline. The fluid washed out was collected and subjected to hypothermic centrifugation (3000 r?min-1, 10 min 4℃) . The supernatant was collected for determination of its protein content. Lung tissue was obtained for determination of myeloperoxidase (MPO) activity, expression of matrix metallo-proteinase-8 (MMP-8) , CrossLaps protein content (the degradation product of type Ⅰ collagen) and wet/dry lung weight ratio(W/D), and microscopic examination. Pulmonary permeability index was calculated (protein content in bronchoalveolar wash-out fluid / plasma protein concentration) .Results At 4, 6 and 8 h after iv LPS administration (in group 3-5) the MMP-8 expression, crosslaps protein content, MPO activity, W/D and lung permeability index were significantly increased as compared with those in control group ( P

Objective To investigate the effects of genistein pretreatment on endotoxin-induced acute lung injury in rats and assess the possible mechanism. Methods Thirty-two male Wistar rats weighing 240-280 g were randomly divided into 4 groups ( n = 8, each group) : group Ⅰ control; group Ⅱ genistein; group ? LPS and group Ⅳ genistein pretreatment. The jugular vein was cannulated for administration of fluid and drug. The animals were anesthetized with intraperitoneal 3% pentobarbital 40 mg? kg-1 . In group Ⅰ and Ⅱ Ⅳ normal saline (NS) 1 ml?kg-1 was Ⅳ given 30min after IP NS 1 ml?kg-1 (Ⅰ ) or genistein 50 mg?kg-1 (Ⅱ ). In group ? and Ⅳ LPS 6 ml? kg-1 was Ⅳ given 30 min after IP NS 1 ml?kg-1 (?) or genistein 50 mg? kg -1 (Ⅳ). Four hours after.LPS injection, rats were sacrificed. The lungs were removed for evaluation of histological injury and determination of wet/dry lung weight (W/D) ratio, myeloperoxidase (MPO) activity, malondialdehyde (MDA) content, expression of TNF-f55 mRNA, HO-1 mRNA, TNF-f55, and HO-1. Bronchoalveolar lavage fluid (BALF) was collected for determination of PMN count, protein content, and MPO activity.Results LPS administration induced marked lung injury and significant increases in W/D ratio, MPO activity, and MDA content in the lung tissues, and PMN count, protein content, and MPO activity in BALF. All of these changes were significantly reduced by genistein pretreatment. Genistein also markedly suppressed LPS-induced expression of TNF-a mRNA and protein, and enhanced LPS-induced expression of HO-1 mRNA and protein. Conclusion Pretreatment with genistein has protective effect against endotoxin-induced acute lung injury. The underlying mechanism is via an inhibition of neutrophilic recruitment and activity, a down-regulation in TNF-f55 production, and a up-regulation in HO-1 expression.

Objective To compare the prognosis effect of different Helicobacter pylori (Hp)eradication methods in patients with chronic gastritis.Methods One hundred and twenty patients with chronic gastritis diagnosed by gastroscopy and pathology examination were divided into sequential therapy group (36 cases),triple-combined therapy group (34 cases),Hp positive without eradication group (30cases) and Hp negative group (20 cases) according to the Hp infection status.All patients were followed up by gastroscopy,biopsy and rapid urease test before and after therapy.Results The Hp eradication rate in sequential therapy group was 94.44％ (34/36),in triple-combined therapy group was 73.53％ (25/34),and there was statistical difference (x2 =5.775,P =0.016).The symptoms scores and gastroscopy scores after therapy in the four groups were significantly lower than those before therapy,and there were statistical differences (P ＜ 0.05).The symptoms scores and gastroscopy scores after therapy in sequential therapy group were significantly lower than those in triple-combined therapy group,and there were statistical differences (P ＜0.05).The symptoms scores and gastroscopy scores after therapy in sequential therapy group,triple-combined therapy group and Hp negative group were significantly lower than those in Hp positive without eradication group [(0.84 ± 0.60),(1.34 ± 0.59),(1.49 ± 0.62) scores vs.(2.98 ± 0.54) scores,(0.47 ± 0.37),(0.83 ± 0.35),(0.96 ± 0.75) scores vs.(1.22 ± 0.40) scores],and there were statistical differences (P ＜ 0.05).Conclusions The Hp eradication rate of sequential therapy is higher than that of triple-combined therapy.The two therapy methods can both improve the symptoms score and gastroscopy score,but the symptoms after sequential therapy are relieved faster compared with triple-combined therapy.

Objective To investigate the effect of Bcl-2 gene knockdown on apotosis , proliferation and drug sensitivity of gastric carcinoma HGC-27 cells. Methods The HGC-27 cells were divided into five groups:the untreated control group , the control siRNA group , the specific siRNA targeting Bcl-2 gene group , 5-FU treated group and the combination group (Bcl-2 siRNA and 5-FU treatment). Then flow cytometry and MTT assays were performed to detect the apoptosis and proliferation of HGC-27 cells. The cysteine protease activityand Cytochrome C release level were tested by ELISA method. Results Bcl-2 knockdown enhanced apoptosis and inhibited proliferation of HGC-27 cells. Comparedwith the 5-FU-treated group , the cell apoptosis level, activities of Caspase-3 and Caspase-9, plasma Cytochrome C were significantly increased in the combination group(P <0.01). Conclusion Bcl-2 gene knockdown induced apoptosis, inhibited cell proliferation and enhanced the drug sensitivity of 5-FU in gastric cancer cells , which might be considered as a potential therapeutic strategy forgastric carcinoma.

Serious troubles were caused by the highly heterogeneous cancer cells in clinical cancer treatment.In recent years,molecular characterization of tumors and corresponding individualized cancer management has become hotspots for cancer research.Circulating tumor cells (CTCs) are shed from primary solid tumors or metastases into the peripheral blood.CTCs could serve as liquid biopsy for patients with cancer,with non-invasive,real-time and repeatable access.Serial monitoring of the amount and molecular characteristics of CTCs in the blood samples has significant clinical value for prognostic prediction,targeted drugs choice and real-time evaluation of clinical effectiveness in individualized cancer treatment.This review summarizes current methods for the CTC isolation and detection,and discusses the perspectives of CTC analyses in real-time personalized cancer therapy.Some future research directions in this field are proposed.

Sepsis associated encephalopathy (SAE)is the most common form of encephalopathy in the pediatric intensive care units and might appear before other systemic features of sepsis.The pathogenesis of SAE is complex and not clear.SAE causes increased morbidity and mortality but has limited therapeutic options.SAE has become a hot issue in critical care medicine.

Breast Neoplasms ; classification ; pathology ; Carcinoma, Ductal, Breast ; classification ; Carcinoma, Intraductal, Noninfiltrating ; classification ; Carcinoma, Papillary ; classification ; Classification ; methods ; Female ; Humans ; World Health Organization