Objective:To establish an HPLC method for the determination of the concentration of ganciclovir in vitro,and to inspect sustain-release of medical biofibrin glue carrying Ganciclovir. Methods:The HPLC system consisted of water-methanol(98:2)as mobile phase with the flowrate of 1.0 ml/min,and the detection wavelength was 252 nm and the injection volumn was 20 ?l. Column tempetature was 30℃. Results:The linear range of ganciclovir was 5~350 ?g/ml(r=0.9996),displaying a good linear relationship. The within-day and between-days relative standard deviations(RSD)were 0.89%～2.35%. The methodological recovery ranged from 98.4 % to 100.8 %. The elaborated method was sensitive,precise and applicable for determining the concentration and release rate of ganciclovir in vitro. Conclusion:The sustain-release effect of medical biofibrin glue carrying ganciclovir in vitro is good.

Objective To investigate the clinical diagnosis and treatment of cosmetics-induced skin lesions.Methods The medical history,clinical manifestation and treatment effects were reviewed in 6230 cases of cosmetic skin damage during 2001-2012.Results The characteristics of cosmetic skin damage included the thinning of the cuticle,glossiness increases and pigment macule during the use of cosmetics time,increased pigment macule after stop and increased blood capillary.A comprehensive treatment was given,including the removal of the causes,correct use of cosmetics,promoting skin metabolism,laser and IPL treatment and maintenance.Through the treatment for 1-2 years,facial cosmetic skin damage returned to normal,and the effects of both doctors and patients were satisfactory.Conclusions Combining with the clinical history,a diagnosis of cosmetics skin damage can be made based on the above 4 characteristics.Comprehensive treatment method is worthy of clinical application.

Animals ; Antioxidants ; pharmacology ; Cerebellum ; cytology ; Dietary Supplements ; Drugs, Chinese Herbal ; chemistry ; Fruit ; chemistry ; Male ; Muscle Fatigue ; drug effects ; Neurons ; drug effects ; pathology ; Oxidative Stress ; drug effects ; Physical Exertion ; Rats ; Rats, Wistar ; Vegetables ; chemistry

Animals ; Female ; Hypertension, Pregnancy-Induced ; metabolism ; pathology ; Kidney ; metabolism ; pathology ; Pregnancy ; Protein Kinase C-alpha ; genetics ; metabolism ; Rats ; Rats, Wistar ; Transcription, Genetic

Acupuncture Therapy ; Female ; Humans ; Huntington Disease ; therapy ; Middle Aged

Objective To study the etiologies of long duration fever in neonates and to provide clinical evidence for early diagnosis and intervention. Methods From January 1st, 2004 to December 31st, 2013, data from neonates admitted to the Department of Neonatology, Childrenˊ s Hospital of Chongqing Medical University were retrospectively analyzed. Results Among 238 neonates with long duration fever, 165 cases were male and 73 female. Pneumonia(n = 196), skin / mucosa infection (n =67), sepsis (n = 46), meningitis/ encephalitis (n = 25) and neonatal necrotizing enterocolitis (n = 24) were the most common etiologies. Diabetes insipidus was the only noninfectious cause of fever in this study. The incidence of high fever in cases diagnosed with meningitis/ encephalitis, sepsis, diabetes insipidus, immunodeficiency or urinary tract infection were significantly higher than other causes (P <0. 05) . Continuous fever or other special fever types were more common in cases diagnosed with pneumonia ( χ2 = 4. 581, P = 0. 032 ) . Incidences of sepsis, infectious diarrhea or meningitis/encephalitis in late neonates were significantly higher than early neonates ( P < 0. 05 ) . Conclusions Long duration fever in neonates is most likely caused by infectious diseases and diabetes insipidus is the only noninfectious cause of neonatal fever in our study.

To emphasize the great function of medical engineering science in the development of sanitarium through analyzing the actual status of medical engineering science in military sanitarium,especially the actual status of management for medical equipment. Starting from the overall management of medical equipment,the reinforcement of medical engineering construction in sanitarium shall ceaselessly develop the connotation of medical engineering and improve the professionalism of medical engineering staff through various ways,so that it can contribute to the healthy,stable and fast development of military sanitarium.

By analyzing the present medical metrology in army medical service units,the importance of medical metrology is emphasized in the development and construction of every unit.In particular,manifold methods should be used to unceasingly improve the professional quality of medical metrology personnel.The development and construction of this subject should be unceasingly expanded in its connotation.It is expected to promote the development of medical service units in a healthy,stable and rapid way by strengthening the construction of medical metrology.

OBJECTIVE: To determine the contents of telmisartan and hydrochlorothiazid in compound telmisartan preparation by TLC. METHODS: Using acetic ether-chloroform-methanol-glacial acetic acid (50∶40∶10∶5) as developer, the contents of telmisartan and hydrochlorothiazid were determined by dual wavelength thin layer chromatography with the reflection linearity parameter SX=7 and slit=1.0 mm?5.0 mm. The determinated wavelength of telmisartan was as follows: ?R=365 nm and ?S=305 nm; the wavelength of hydrochlorothiazid was as follows: ?R=370 nm and ?S=275 nm. RESULTS: The linear ranges of telmisartan and hydrochlorothiazid were 1.114～5.57 ?g (r=0.999 6) and 3.664～18.32 ?g (r=0.999 8), respectively, and the average recovery rates of telmisartan and hydrochlorothiazid were 97.95% (RSD=1.30%) and 98.32% (RSD=1.13%), respectively. CONCLUSION: The method is simple, rapid, accurate and it can be used for the quality control of compound telmisartan preparation.

The emphasis and basic target of medical education is to cultivate medical students with high qualities. The qualities of medical students include professional ethics, self-cultivation, medical knowledge and physical-psychological quality, which is critical to the medical education. It has been proven that the teaching of Clinical Pathway is one of the most important factors to promote the improvement of comprehensive qualities of medical students.