Atrial tumor myocytes (AT-1 cells) were derived from tumor-bearing mice in which SV40 T large antigen drives atrial natriuretic promoter. AT-1 cells possess many features typically seen in cardiac cells from other species, such as spontaneous beating, multiple ion channels and responses to drugs, etc. Major ion channels are present in AT-1 cells: sodium, calcium and potassium channels. Sodium channel in AT-1 cells is highly sensitive to blocker tetrodotoxin. L- and T-type calcium channels respond to classical blockers. An important outward potassium current in AT-1 cells is the rapidly-activating delayed rectifier (IKr). IKr Is the sole time-dependently repolarizing potassium current in AT-1 cells. AT-1 cells are a very useful tool for studies in cardiac electrophysiology and pharmacology. As yet, a great number of cardiac and non-cardiac drugs have been tested for their IKr blocking actions in AT-1 cells. The polymorphic ventricular arrhythmia (torsade de pointes) is a severe side effect of IKr blockers.

Objective To compare of pregnancy induced hypertension caused postpartum hemorrhage by two treatment methods. Methods 104 patients with pregnancy induced hypertension were randomly divided into misopr-ostol plus oxytecin group ( observation group)54 cases and the control group ( 50 eases ), used oxytcoin alone, respec-tively, Results postpartum hemorrhage in The observation group of is five cases(9.2% )which was significantly low-er than the control group of 11 cases(22.0% ) ( X2= 3.95 ,P<0.05 ). average postparttun hemorrhage in observation group is ( 195.6±88.4) ml which was significantly lower than the control group (303.2±111.6) ml (t=2.31, P<0.05). Conclusion Misoprostol combined with oxytocin is effective, safe and convenient to patients with postpartum hemorrhage caused by pregnancy induced hypertension.

Objective To evaluate the efficacy of recombinant tissue-type plasminogen activator(rt-PA)and mild hypothermia for acute cerebral infarctions. Methods72 patients consistent with the inclusion criteria were randomly divided into 2 groups,hypothermia group receivied rt-PA combined with mild hypothermia and control group receiving rt-PA only.Neurological functions was evaluated with European Stroke Scale(ESS). ResultsAs intravenous thrombolytic therapy within 6h after the onset,the ESS scores examined 3 days after thrombolysis in hypothermia group was significantly higher than those in control group.As intravenous thrombolytic therapy during 6h to 12h after the onset,the ESS scores of hypothermia group increased more significantly from 24h after thrombolysis than those in control group. Conclusionft-PA combined with mild hypothermia was more effective than rT-PA only for acute cerebral infarctions,which was effective and relatively safe in the treatment of acute ischemic cerebral vascular disease.

Objective:To study the influence of cusp inclination of artificial posterior teeth on abutment displacement of unilateral distal-extension telescope crown denture.Methods:Study models of mandibular unilateral distal-extension and telescope crown dentures with the cusp inclination of 0?,12?,25? and 33? were respectively prepared. When food chip was masticated on the occlusal surface and the dentures were loaded by 29.4 N, 49 N or 68.6 N, the abutment displacement was measured.Then the data were calculated and reciprocally compared with statistical analyses. Results:Between anatomic and 25? semi-anatomic artificial posterior teeth, there were significant differences in the buccolingual and vertical abutment movement(P0.05). The parallel, inclination, twist-like and vertical movement of abutment increased with the increase of the load on the denture. The difference among different level of load was statistically significant (P

The emphasis and basic target of medical education is to cultivate medical students with high qualities. The qualities of medical students include professional ethics, self-cultivation, medical knowledge and physical-psychological quality, which is critical to the medical education. It has been proven that the teaching of Clinical Pathway is one of the most important factors to promote the improvement of comprehensive qualities of medical students.

Ophthalmology is a clinical course which requires students to have practical skills. The traditional clinical teaching method lacks varied means resulting in unsatisfactory teaching effects. Application of multimedia technology not only changes the teaching model, enriches the teaching contents but also improves the quality of ophthalmology teaching. By analyzing the advantages and disadvantages of multimedia application in clinical teaching based on our practice, the paper raises some methods for improvment in order to achieve the best teaching effects.

This study was aimed to explore the nonlinear relationship between syndromes and prescriptions by using the artificial neural network (ANN) algorithm. The clauses composed of syndromes and prescriptions in Shanghan Lun (Treatise on Cold Damage and Miscellaneous Diseases) were sorted out. A total of 245 clauses were trained with ANN to establish the knowledge base model of syndromes and prescriptions. After that, 100 clauses were tested. And then, the symptoms of Gui-Zhi-Tang (GZT) syndrome, Ma-Huang-Tang (MHT) syndrome, Xiao-Chai-Hu-Tang (XCHT) syndrome and Bai-Hu-Tang (BHT) syndrome were input into the model to predict the Chinese herbs. The results showed that the test accuracy of the model was 79%. For the herbs to GZT and MHT syndrome, the test error was less than 0.1. For the main herbs to XCHT and BHT syndrome, the test error was less than 0.1. While for other herbs, the test error was less than 0.3. It was concluded that the ANN algorithm can simulate the nonlinear relationship between syndromes and prescriptions, which can be applied to study the syndrome differentiation, in order to contribute to the syndromes and prescriptions standardization and the traditional Chinese medicine (TCM) information.

OBJECTIVE To investigate the pathogens of HAP and their drug resistance in our hospital,the impact of duration of hospitalization on etiology,and the risk factors for the resistant pathogens.METHODS The respiratory tract secretion was collected from HAP patients to culture and analyze the susceptibility of antimicrobials,compare the etiology of early-onset and late-onset HAP including prior antibiotics exposure and severity of HAP to drug resistant bacteria.RESULTS During the period of study,there were 1.04% of patients suffering from HAP.Early-onset HAP was commonly caused by G+ cocci while the main agents of late-onset HAP were G-bacilli.Most of the HAP pathogens were resistant to the antimicrobials commonly used in clinic with the prior antimicrobials exposure and the severe HAP increased the resistant bacteria rate(P

OBJECTIVE:To discuss the approach of drug pricing by applying the pharmacoeconomics evaluation.METHODS:Theory deductions together with demonstration analysis were performed based on the theory of pharmacoeco-nomics.RESULTS:In drug pricing for new drugs,the pharmacoeconomic evaluation should be conducted with suitable ref-erence drugs and suitable reference prices as references,meanwhile based on the results,the reported costs-based price should be reevaluated and adjusted accordingly.CONCLUSION:The rationality of drug pricing can be effectively improved through pharmacoeconomics evaluation.

Objective To evaluate the hospitalization charges of medical-insured patients with different in-comes, different age and the burdens shared by individuals, to analyze the multiple factors that influence the charges and provide basis for goverment policy making. Method We investigate the medical costs and incomes of 1588 medi-cal-insured inpafients in a Nantong hospital ,study the expenditure burdens and influencing factors of patients with dif-ferent income and different age appling T-test,F-test,and χ2 test. Results There is singificant difference in expendi-ture burdens between patients of different income level ,the expenditure burdens are obviously heavier in group of old age patients than in other age groups. Conclusion It is necessary to reinforce the government functions and perfect the health security system, low income groups should be given more attentions and cares.