Objective:To establish an HPLC method for the determination of the concentration of ganciclovir in vitro,and to inspect sustain-release of medical biofibrin glue carrying Ganciclovir. Methods:The HPLC system consisted of water-methanol(98:2)as mobile phase with the flowrate of 1.0 ml/min,and the detection wavelength was 252 nm and the injection volumn was 20 ?l. Column tempetature was 30℃. Results:The linear range of ganciclovir was 5~350 ?g/ml(r=0.9996),displaying a good linear relationship. The within-day and between-days relative standard deviations(RSD)were 0.89%～2.35%. The methodological recovery ranged from 98.4 % to 100.8 %. The elaborated method was sensitive,precise and applicable for determining the concentration and release rate of ganciclovir in vitro. Conclusion:The sustain-release effect of medical biofibrin glue carrying ganciclovir in vitro is good.

Objective To investigate the clinical diagnosis and treatment of cosmetics-induced skin lesions.Methods The medical history,clinical manifestation and treatment effects were reviewed in 6230 cases of cosmetic skin damage during 2001-2012.Results The characteristics of cosmetic skin damage included the thinning of the cuticle,glossiness increases and pigment macule during the use of cosmetics time,increased pigment macule after stop and increased blood capillary.A comprehensive treatment was given,including the removal of the causes,correct use of cosmetics,promoting skin metabolism,laser and IPL treatment and maintenance.Through the treatment for 1-2 years,facial cosmetic skin damage returned to normal,and the effects of both doctors and patients were satisfactory.Conclusions Combining with the clinical history,a diagnosis of cosmetics skin damage can be made based on the above 4 characteristics.Comprehensive treatment method is worthy of clinical application.

Animals ; Antioxidants ; pharmacology ; Cerebellum ; cytology ; Dietary Supplements ; Drugs, Chinese Herbal ; chemistry ; Fruit ; chemistry ; Male ; Muscle Fatigue ; drug effects ; Neurons ; drug effects ; pathology ; Oxidative Stress ; drug effects ; Physical Exertion ; Rats ; Rats, Wistar ; Vegetables ; chemistry

Objective To investigate if the effects of integated traditional Chinese and westetrt medicine treat ing diabetic osteoporesis.Methods 120 patients aging from 50 to 70 years were randomly recruited into two groups according to theirgender,namely DN1 and DN2.DN1 groupwastreatedwitll vitamin D3 and DN2 groupwastreatedwitll vitaminD3 plus Tanggukang capsule.Results X-ray showed that the bone density in the DN2 group WaS higher than DNI group,and the difference was significant(P＜0.05).Conclusion The integrated traditional Chinese and western medicine has good effects in treating diabetic osteoporosis.

Objective: To evaluate the development, safety and efficacy of percutaneous nephrolithotomy(PNL) for management of upper urinary tract calculi in pediatric patients.Methods: In the study, 77 pediatric patients undergoing 87 PNLs through mini or standard tract for upper urinary tract calculi between January 2005 and December 2016 in Peking University People''s hospital were reviewed, including 69 renal calculi, 6 upper ureteral calculi, 12 renal and upper ureteral calculi, 35 single calculi, 43 multiple calculi and 9 staghorn calculi.The development and efficacy of PNL in pediatric patients were studied by analyzing the characteristics and clinical indexes, and by reviewing the associated literature.The Clavien classification system was used to evaluate the complications after PNL.Results: A total of 87 PNLs were performed in 77 pediatric patients.Eighty-one upper urinary tract calculi were managed through a single tract(93.1%), 5 pediatric patients were managed through 2 tracts(5.7%), and 1 pe-diatric patient was managed through 3 tracts(1.2%).The mean operating time was (77.0±29.8) min.The stone-free rate after one session was 100% for single calculi and 71.2% for multiple or staghorn calculi, 5(5.8%) children underwent auxiliary procedure to remove the residual calculi and the final stone-free rate of PNL was 88.5%.One of the main complications of pediatric PNL was fever.Sixteen(18.4%) had moderate fever(38-39 ℃), 5(5.7%) had high fever(39-40 ℃) and there were no severe complications of infection, such as sepsis or septic shock.The mean hemoglobin loss was (10.3±16.1) g/L and the serum creatinine rise was (7.0±13.3) μmol/L.One(1.2%) pediatric patient suffered ureteroscopic lithotripsy because of the obstruction by the residual stone in ureter.No injury of organs or retroperitoneal urinary extravasation occurred.General assessment of the complications showed Clavien grade Ⅰ complications in 14 (16.1%) pediatric patients, grade Ⅱ in 7(8.0%) children and grade Ⅲ in 1(1.2%) children.There was no grade Ⅳ or Ⅴ complications.The overall complication rate was 25.3%.Conclusion: PNL for management of upper urinary tract calculi in pediatric patients is effective.Complications after PNL, as assessed with Clavien classification system, are mild and PNL in pediatric patients is safe.

By analyzing the present medical metrology in army medical service units,the importance of medical metrology is emphasized in the development and construction of every unit.In particular,manifold methods should be used to unceasingly improve the professional quality of medical metrology personnel.The development and construction of this subject should be unceasingly expanded in its connotation.It is expected to promote the development of medical service units in a healthy,stable and rapid way by strengthening the construction of medical metrology.

SUMMARY Lymphoma is a systemic disease. It is not uncommon to be found involved in digestive or central nervous system. However, lymphoma involved in these two systems at the same time is rare. The clinical feature of a case of lymphoma with gastrointestinal bleeding and limbs weakness was investigated and the literature was reviewed. The patient came to our hospital with melena and hematemesis. She was diagnosed as gastric ulcer by gastroscopy and biopsy showed lymphoma. Two days after she came to hospital, the patient presented with progressing limbs weakness. Magnetic resonance imaging (MRI) showed irregular abnormal signals in T2-T4 vertebra, which was enhanced obviously. A strip abnormal signal could be seen in spinal cord and involved in neighboring centrum and ribbing. The lesion extended to paravertebral tissue. The final diagnosis was lymphoma involved in stomach and spinal cord. Diseases presented with both upper digestive tract bleeding and symptoms of central nervous system were rare, including malignancies, virus infection and some therapy. Lymphoma was one of the causes. On the other hand, spinal cord ischemia might occur after gastrointestinal bleeding. Thus, doctors should examine the patients carefully to diagnose these diseases.

OBJECTIVE: To determine the contents of telmisartan and hydrochlorothiazid in compound telmisartan preparation by TLC. METHODS: Using acetic ether-chloroform-methanol-glacial acetic acid (50∶40∶10∶5) as developer, the contents of telmisartan and hydrochlorothiazid were determined by dual wavelength thin layer chromatography with the reflection linearity parameter SX=7 and slit=1.0 mm?5.0 mm. The determinated wavelength of telmisartan was as follows: ?R=365 nm and ?S=305 nm; the wavelength of hydrochlorothiazid was as follows: ?R=370 nm and ?S=275 nm. RESULTS: The linear ranges of telmisartan and hydrochlorothiazid were 1.114～5.57 ?g (r=0.999 6) and 3.664～18.32 ?g (r=0.999 8), respectively, and the average recovery rates of telmisartan and hydrochlorothiazid were 97.95% (RSD=1.30%) and 98.32% (RSD=1.13%), respectively. CONCLUSION: The method is simple, rapid, accurate and it can be used for the quality control of compound telmisartan preparation.

The emphasis and basic target of medical education is to cultivate medical students with high qualities. The qualities of medical students include professional ethics, self-cultivation, medical knowledge and physical-psychological quality, which is critical to the medical education. It has been proven that the teaching of Clinical Pathway is one of the most important factors to promote the improvement of comprehensive qualities of medical students.

Ophthalmology is a clinical course which requires students to have practical skills. The traditional clinical teaching method lacks varied means resulting in unsatisfactory teaching effects. Application of multimedia technology not only changes the teaching model, enriches the teaching contents but also improves the quality of ophthalmology teaching. By analyzing the advantages and disadvantages of multimedia application in clinical teaching based on our practice, the paper raises some methods for improvment in order to achieve the best teaching effects.