Animals ; Female ; Hypertension, Pregnancy-Induced ; metabolism ; pathology ; Kidney ; metabolism ; pathology ; Pregnancy ; Protein Kinase C-alpha ; genetics ; metabolism ; Rats ; Rats, Wistar ; Transcription, Genetic

Cytomegalovirus(CMV) is an important pathogen of congenital and postnatal infections in children,which causes a series of acute and chronic infectious diseases and nervous system sequelaes.Early and accurate diagnosis of pediatric CMV infection is an effective way to improve health in children.This paper will introduce the types,laboratory techniques and diagnostic strategies of CMV infection based on the diagnostic standards at home and abroad,and also focus on current progress in diagnosis of pediatric CMV infection.

The article reviews the change of thyroid function in the women during pregnancy and the development of fetal thyroid function and introduces the principles of treating pregnancy with hyperthyroidism or hypothyroidism.

Objective To discuss the clinical features of dystonia manifested as Parkinsonism ( PKS) . Methods Clinical materials of a patient with dystonia manifested as PKS were analyzed retrospectively. Results The onset age of the young women was 31 years old, who was started asymmetrically with symptoms of claudication and tremor of the right foot. Levodopa had a short-term effect. The results of dopamine transporter ( DAT) PET showed that DAT in retrolentiform part were decreased significantly. Atypical Parkinson's disease was considered and she was treated as PKS long-termly. Subsequently, heterozygous mutation of c. 268-4T>A (NM_018105) in DYT6 gene was found through the next-generation sequencing, which was a kind of splicing mutation and confirmed by the first-generation sequencing. Conclusions Patients with dystonia might share similar clinical manifestations with PKS. Particularly, they should be differentiated with young-onset Parkinson's disease combined with focal dystonia. Clinical observation and genetic testing are important approaches to differentiate them.

AIM: Theaflavins are the major constituent of black tea, and have a lot of pharmacological activities, for example, regulating blood fat, anti-oxygen, anti-tumor, resisting cerebrovascular disease, and so on. Previously, theaflavins' effect on human fat metabolism mostly belongs to macro-analysis, but this experiment try to find out the effect of theaflavins on the differentiation of rabbit mesenchymal stem cells (MSCs) into adipocytes in terms of cells. METHODS: The experiment was completed in the center laboratory of Anhui Provincial Hospital Affiliated to Anhui Medical University from May to September in 2007.①Ten rabbits of cleaning grade and 4-8 weeks old were provided by the animal experiment center of Anhui Medical University. The disposal of animals in process of experiment referred to the ethical standard of animals.②The heparinized rabbits were sacrificed in drugged state, then bilateral femur, tibia and humerus were obtained and their soft tissues were removed, cutting epiphysis of two sides including epiphyseal plate. The MSCs were isolated and cultured with whole bone marrow culture method, inoculated at a density of 2?108 L-1. When the cells grew to the fusion of 80%-90%, digesting and passage culture were performed. Cells of the third generation were collected and inoculated at a density of 1?105/cm2 to be cultured for 2 weeks with DMEM adipocyte-induced liquid, which contained recombinant human insulin 10 mg/L, dexamethasone 10-6 mol/L, and IBMX 0.5 mmol/L. Three groups were set up: adipocytes control group in 1 mL adipocyte-induced liquid; theaflavins group with the 500 ?g/L theaflavins in 1 mL adipocyte-induced liquid; blank group in equal amount of ordinary DMEM culture medium.③The cultured cells of the second generation were selected to draw growth curve; oil O staining was used to identify the induced adipocytes and calculate the differentiation efficiency of adipocytes. RESULTS: ①Cell growth curve: MSCs had vigorous reproductive activity, 1 day of the culture was the cell adaptive phase, 3 days was the increased logarithmic phase, and 8 days was the platform phase. After 8 days, cell proliferation quickly stepped down and cells reduced.②Identification of adipocytes by oil O stain: Lipid droplets in adipocytes were stained into salmon pink, and the nucleus was stained into blue. Most of MSCs in adipocyte control group were induced into adipocytes, the differentiation efficiency was (64.8?4.8)%, while that in theaflavins group was only (32.0?3.4)%. No adipocytes obviously formed in blank control group. CONCLUSION: Theaflavins can obviously inhibit differentiation from rabbit MSCs to adipocytes.

Objective To investigate the relationship between the leptin level and neonatal weight and the expression of leptin in placenta. Methods The concentrations of leptin in 100 maternal blood and umbilical blood of the term pregnant women were examined by radioimmunoassay (RIA). According to the neonatal weight to divide into the large for gestational age (LGA) group 19 cases, the appropriate for gestational age (AGA) group 65 cases, the small for gestational age (SGA) 16 cases. The level of leptin mRNA in 41 placental tissue was examined by Reverse transcription polymerase chain reaction (RT PCR). Results (1) The expression level of leptin mRNA in placenta was 0 97?0 04, which was positively related to the neonatal weight significantly ( r =0 43, P 0 05).(3)The concentration of leptin in umbilical blood was (7 58? 5 15) ?g/L, which was positively related to the neonatal weight ( r =0 57, P

Objective To determine the relation between the expression of urocortin and corticotropin-releasing hormone receptor 2? (CRH-R2?) in the placenta and pathogenesis of preeclampsia. Methods Placentas were collected from 20 pregnant women with preeclampsia as study group and 20 normal pregnant women as control group. Urocortin mRNA and CRH-R2? mRNA were measured by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR). Urocortin peptide was measured by immunohistochemistry. Results (1) The mRNA expression of urocortin was significantly higher (P

Objective To study the change of interleakin-18 (IL-18) in patients of pregnancy induced hypertension syndrome(PIH) and its correlation with endothelint-1 (ET-1). Methods Serum levels of IL-18 and plasma levels of ET-1 in 47 PIH patients and 30 normal pregnancy women were measured with ELISA and RSA respectively. Results (1)Serum IL-18 of PIH group (M=43.76 ng/L) was significantly higher than that of normal pregnancy group (M=37.975 ng/L) , H=42.845,P

Objective To address angiotensin Ⅱ type 1 receptor (AT 1R) gene polymorphism and its relationship with plasma endothelin (ET) in PIH. Methods Gene polymorphism of AT 1R at site 1166 was detected with polymerase chain reaction (PCR), digestion of restriction enzyme and electrophoresis. Plasma ET was measured by radioimmunoassay. Results 1. The frequencies of genotype(AC and CC) at 1166 of AT 1R gene and C allele in PIH individuals were significantly higher than those of control group, (? 2=11.06, 9.73, respectively, P0.05). Conclusions 1. The variant (A→C) of 1166 polymorphism site of AT 1R is associated with the generation of PIH, and C allele may be a susceptible gene to PIH. 2.There is relationship between AT 1R gene and plasma ET level in PIH patients.