Objective To investigate the influences of carbon dioxide (CO2) pneumoperitoneum and operation position on intraocular pressure (IOP) during laparoscopic surgery under general anesthesia. Methods Thirty-six patients were divided into two groups of udergoing laparoscopic pelvic surgeries (group A) and udergoing laparoscopic cholecytectomies(group B) with 18 cases each. The patients in group A were operated under 25 degrees head-down position and those in group B under 25 degrees head-up position. IOP was recorded before surgery(T0), at 5 min after tracheal intubation (T1), 5 min after CO2 pneumoperitoneum(T2), 5 min(T3) and 30 min(T4) after body position changed and the time of normal PETCO2 regained(T5). Results IOP was remarkably decreased after induction of general anesthesia in both groups. IOP of group A raised to (21.0±1.6) mm Hg at T4, which was dropped to (14. 6±1.6) mm Hg at T5. IOP of group B raised to (12.1±2.9) mm Hg at T4,which was dropped to (11.1±1.2) mm Hg at T5. Changes of IOP of both groups were positively correlated with the changes of PETCO2. Conclusion IOP can be decreased remarkably after induction of general anesthesia with propofol. The head-up position and normal range of PETCO2 are the two important factors to avoid an increase of IOP in surgical patients under general anesthesia.

Ocular surface diseases are common diseases in ophthalmology.It is difficult for clinicians to make early and accurate diagnosis based on symptoms, signs and serologic examination.Compared with the blood test, the tear test can reflect the condition of the local ocular surface and it has the characteristics of being non-invasive, having high diagnostic accuracy and fast diagnostic speed.Tear detection uses modern detection techniques such as polymerase chain reaction, enzyme-linked immunosorbent assay, gene chip and protein chip to detect the pathogen and immune components in tear, including microbial nucleic acid, tear antibody (IgM, IgG, IgE, IgA, antinuclear antibody, etc.) and cytokines (interleukin, tumor necrosis factor, interference factor, transforming growth factor, epidermal growth factor, etc.). Tear detection provides information about the pathogen infection and immune response on the ocular surface.At present, a large number of domestic and foreign tear detection studies show that there are certain regular changes in tear composition, not only in ocular surface diseases, uveitis, fundus diseases, thyroid related ophthalmopathy, but even in systemic diseases such as diabetes, hepatitis B, acquired immune deficiency syndrome etc.In addition, the research of tear pathogen and immune components is of great significance to study the cause, biochemical and immune process, treatment of diseases.In this paper, the latest research progress of tear collection, the clinical significance of tear pathogen and immune components and the characteristics of tear in various diseases was summarized and analyzed to provide reference for clinical practice.

Objective To evaluate the efficacy of seretide (fluticasone/salmeterol) in treatment of patients with cough variant asthma(CVA). Methods 86 CVA patients were treated with fluticasone/salmeterol propionate 8 weeks, after 8 weeks, 86 patients were randomly assigned to treatment group and control group, each group with 43 patients, the treatment group continued medicine for 4 weeks, the control group without any treatment.The two groups of patients FENO measurement values, cough symptom score before treatment and after treatment for 8 weeks, 12 weeks were compared. Results After treatment for 8 weeks, cough symptom scores and FeNO in the two groups values decreased significantly compared with those before treatment(P<0.05), 12 weeks after treatment, treatment group cough product, FeNO values, were lower than in control group(P<0.05).Conclusion Fluticasone salmeterol powder inhalation can effectively improve the symptoms of cough cough variant asthma.Symptom score and FENO value can be used as a monitoring index to evaluate the therapeutic effect of CVA, and the FENO value is more effective, which has the advantages of non-invasive, simple, safe, rapid and repeatable.

Child ; Desensitization, Immunologic ; Humans ; Rhinitis, Allergic ; Rhinitis, Allergic, Perennial ; therapy

Air Pollutants ; analysis ; Dust ; analysis ; Environment Design ; Environmental Monitoring ; instrumentation

Objective To investigate the efficacy and side effects of new combination of high-dose methotrexate (MTX),cisplatin (DDP),pirarubicin (THP),and ifosfamide (IFO) in the treatment of extremity osteosarcoma patients.Methods A retrospective analysis was conducted for 125 osteosarcoma patients treated with the optimized neo-adjuvant chemotherapy combined with the four drugs mentioned above (MTX 200 mg/kg iv 6 h d1,CF 9 mg at one time,12 times; DDP 100 mg/m2,THP 30 mg iv,d8-10;IFO 3.0 g/m2 iv d16-18,mesna after IFO for 0,3,6,9 h).The efficacy and side effects of the therapeutic scheme were evaluated.Results The effective salvage rates of Ⅱ a and Ⅱb schemes were 36.4％ (8/22) and 79.6％ (82/103) with a statistical significance (P ＜ 0.05).The 2-year survival rate of schemes was 62.4％,and the survival time was(17.7 ± 8.3)months.The main side effects were myelo-suppression,nausea,and vomiting,etc.The incidence rate of myelo-suppression,nausea,and vomiting with grade Ⅲ-Ⅳ accounted for 71.2％,37.6％,and 13.6％,respectively.Conclusions The optimized neo-adjuvant therapy strategy based on high-dose MTX,DDP,THP,and IFO is effective in the treatment of osteosarcoma.

Objective To establish the quality standard of Alatan Wuwei Pill. Methods Chebulae Fructus and Granatii Fructus were identified by TLC. Gallic acid and ellagic acid in Gardeniae Fructus and Granatii Fructus were determined by HPLC. Results The TLC spots developed were clear. Gallic acid showed good linear relationship in the range of 0.058 72-1.056 96 mg (r=1.000 0), the average recovery was 99.15%(RSD=1.3%). Ellagic acid showed good linear relationship in the range of 0.079 64-1.194 6 mg (r=1.000 0), the average recovery was 100.02% (RSD=2.3%). Conclusion The method is simple and reproducible. It can be used to control the quality of Alatan Wuwei Pill.

BACKGROUND:At the beginning of the 20th century a strength training method in the backward direction appeared:hip stress strength training method. There are many articles focusing on the concept, function, effect and mechanism of this training method. So far, relevant research has formed a unique branch of special strength training research directions.
OBJECTIVE:To conclude the concept, function, effect, features and mechanisms of hip stress technical strength training through the systematic analysis of literature concerning hip stress strength training. METHODS:CNKI (1998/2010) and Wanfang databases were searched by the first author for relevant articles. The keywords were“hip stress, strength training, trajectory control, exercise training, muscle strength, knee, physical fitness, bone”in Chinese and English. The obtained data were summarized using contrast method and logic analysis method.
RESULTS AND CONCLUSION:Total y 90 papers were retrieved, and final y 34 papers were included. The results showed that the hip technical strength training method is the most effective training method currently to cultivate the running, jumping and throwing back pedaling force after hip extension forces. It has a new training idea and special training equipments, which can effectively solve many sports difficulties in strength training and technical training and has the vital significance for the high-level sports training, skil s assessment and sports teaching. Hip stress special technical strength training research has formed a special research branch of special strength trainings, which wil play a more significant research role.

[Objective] To compare the expression of matrix metalloproteinase 2(MMP-2)and matrix metalloproteinase 9(MMP-9)induced by alumina and ultra-high-molecular-weight polyethylene(UHMWPE)wear particles,and investigate the role of MMP-2 and MMP-9 in the mechanism of the periprosthetic osteolysis induced by wear debris during aseptic loosening.[Methods]Seventy-two Kunmin mice were divided into 3 groups(24 per group)in a murine air pouch model of inflammation.The air pouches were injected with 3ml of suspension containing 1?108 /ml alumina particles in group A,while UHMWPE particles in group B,and control pouches received 3ml of sterile PBS.All animals were sacrificed at 3、7、14 days after injection.Histological changes and cell counting were assessed by light microscope.RT-PCR and immunohistochemistry method were applied to detect the gene and protein expression of MMP-2 and MMP-9 in the pouches.[Results]Increased cell infiltration and MMP-2,MMP-9 mRNA and immunoreactivity expression were detected in group A and B,and compared to control group,cell number and gene and protein expression of MMP-2 and MMP-9 in group A and B was significantly higher(P

Objective:To understand the liability situation of urban public hospitals in Jiangxi.Methods:Qualitative and quantitative analysis,descriptive analysis and comparative analysis were used to analyze the causes and changes of urban public hospitals' debt increase.Results:To meet the increasing demand for health diagnosis and services,debt infrastructure and the purchase of equipment coupled with inadequate financial investment were performed in public hospitals,which resulted in the sustainably increasing of liabilities level in urban public hospitals.Conclusion:Governments at all levels should increase the capital expenditure of hospital construction and equipment investment,strictly control the public hospital debt behavior,the implementation of the reform of public hospitals canceled drug addition,establishing the characteristics of the medical industry personnel salary system and other measures to resolve the synchronous speed of public hospital debt and prompt public hospital debt risk control in the reasonable scope.