OBJECTIVE: To evaluate the status quo and the tendency of utilization of antihypertensives in our hospital. METHODS: The consumptions of antihypertensives from 2001 to 2004 were analyzed statistically with Microsoft Excel 2000. RESULTS: The use of antihypertensives assumed an increased tendency, of which, about 90% were included in the catalog of the Drugs of National Basic Hospitalization Insurance. Calcium antagonists, diuretics, ACEI and ?-receptor blocking agents were the basic clinical drugs. Usage of angiotensin;angiotensin Ⅱ receptor blocking agents increased to a large degree year by year. CONCLUSION: The utilization structure of antihypertensives in our hospital is basically reasonable. Developing compound antihypertensive that of excellent quality and reasonable price is of great significance in the management of hypertension and its complications.

Objective To investigate the clinical characteristics ,pathogenic bacteria detection rate and drug sensitivity situation of pulmonary infection in surgical trauma inpatients .Methods The characteristics ,pathogenic clinical examination and treatment method of pulmonary infection among traumatic inpatients in Fengdu County Hospital of Traditional Chinese Medicine from June 2013 to December 2014 were retrospectively analyzed .Results The clinical symptoms of pulmonary infection were usually atypical . The laboratory detection showed 31 cases of WBC>10 .0 × 109/L ,32 cases of (4-10)× 109/L ,0 case of <4 .0 × 109/L ,and 55 ca‐ses of neutrophils proportion>70% ,8 cases of neutrophils proportion 50% -70% and 0 case<50% .51 cases (81% ) were definite in pathogenic diagnosis ,in which 46 cases were obtained by sputum culture and 12 cases were obtained from blood culture .Lung in‐fections were mainly caused by bacterial infections (68 .3% ) ,which was nominated by Gram‐negative bacteria ,including 15 cases of single bacterial infections ,23 cases of complex bacterial infection and 15 cases of complicating fungal infection .Conclusion The treatment success rate of surgical trauma patients is affected by the combined action of lung infection and complicated drug resist ‐ance ,so the clinical examination plays a very important role .

AIM: To evaluate Ribbond ultrahigh molecular weight polyethylene fiber (UMWPF) on hamster's pouch by oral membrane irritation. METHODS: Experiments were performed at the Animal Experimental Center of Henan Province from July to December 2004. Ten healthy male golden hamsters aged 60-70 days were selected. The samples of UMWPF, positive and negative control group (n=10, diameter 4 mm, thickness 1 mm) were fixed on the oral membrane of hamster's pouch with medical thread. The thread should pass through membrane and fixed on the animals' skin. The experimental samples were on the one side of pouch membrane (UMWPF toward membrane); the positive and negative samples were on the cephalic and tail of the other side respectively. After two weeks' systemic and local observation, the local oral membrane specimens were viewed under microscope. RESULTS: Totally 10 hamsters were included in the final analysis. ①2 weeks after the surgery, the animals pouch membrane contacting with the experiment and negative samples showed no hyperemia, hydrops, erosion or ulcer, and where positive samples contacted showed rough; 7 of them showed light hyperemia and hydrops. ②The epithelia layer of the animal membrane contacting with experiment and negative samples were complete, which cells were in order, epithelial processes stretched to the basal layer. There was no inflammatory cells infiltration, congestion or edema in the connective tissue, but which showed contrast in the positive group. ③The epithelial cells of the experiment and negative groups had no changes in morphology, while in the positive group a few cells swelled and their nucleolus were more clear than normal, and their shapes were circle which was different from normal. There were no systemic or local abnormal response to UMWPF and that the histopathological findings were also normal. CONCLUSION: Ribbond ultrahigh molecular weight polyethylene fiber has no abnormal irritating response to oral membrane of golden hamster.

Objective:To survey and evaluate the situation of present clinic use of antidiabetic drugs,estimate their consuming trend and discuss the rational methods in applying antidiabetic drugs.Method:The drug purchasing data during the years of 2002 to 2006 in hospitals were compared,and statistically analyzed with Microsoft Excel.Result:Ac- counting approximately 52%-72% in total antidiabetie DDDS,sulfonylureas and bigunides were most commonly used.In- sulin and?-Glycosidase inhibitors also took more than 25%-35% share in total DDDs.Some other new drugs and tradi- tional Chinese medicine were less than 10% in total DDDs.The first line of antidiabetic drugs included mefformin,gliclas- ide,acarbose,glipizide and insulin.Conclusion:Clinical application of antidiabetic drugs was reasonable in Nanjing dis- trict.The cost of diabetes patient is the main factor which will influence the application of antidiabetic drugs normatively and continuously.

Objective:To study the perioral soft tissue characteristics of the subjects with ClassⅡDivisionⅠand various vertical pat-terns. Methods:Lateral cephalograms of 90 adults ( half men, half women) were divided into 3 groups based on GoGn-SN angle:low angle, normal angle and high angle(n=30). The perioral soft tissues were measured and compared for the determination of the vertical skeletal variables influencing soft tissue characteristics. Results: The subjects with low angle showed significantly smaller values than those with high angle for basic lower lip thickness and lower lip length(P<0. 05). Most measurements of soft tissue thickness were greater in men than in women in all groups, especially for basic upper lip thickness and upper lower lip thickness(P<0. 05). Conclu-sion:Orthodontists need to pay more attention to the differences of perioral soft tissue in the subjects with various vertical patters to ob-tain balance of the facial harmony in orthodontic treatment.

In view of the main problems of Matlab teaching of biomedical engineering profession,the paper proposes main measures for teaching reforming,including the adjustment of teaching hours,teaching content,teaching materials and other aspects,as well as reform of teaching methods and examination.The investigation result shows that the reform has a positive meaning on the improvement of students' learning quality.

Objective To assess the antimicrobial resistance and resistance-related mutations in rpoB and katG genes in Mycobacteria tuberculosis isolates from patients with cutaneous tuberculosis. Methods Seven strains of Mycobacteria were isolated from lesions or secretions of patients with cutaneous tuberculosis and identified as M. tuberculosis. Proportion method was used to test the susceptibility of M. tuberculosis isolates to rifampicin, isoniazid, streptomycin and ethambutol. PCR and sequencing were performed to analyze the mutations in rpoB and katG genes. Results Of the 7 isolates of M. tuberculosis, 1 was resistant to rifampicin,isoniazid and ethambutol simultaneously, and the other 6 were sensitive to rifampicin, isoniazid, streptomycin and ethambutol. All the 7 isolates were positive for the amplification of rpoB and katG genes by PCR. DNA sequencing revealed two mutations at codon 531 (TCG to TTG) and codon 315 (AGC to ACC) in the multi-drug resistant strain, which were absent in the other 6 strains. Conclusion Multi-drug resistance has emerged in M. tuberculosis isolates from patients with cutaneous tuberculosis, which is likely to be related to improper treatment.

Objective To study the clinical characteristics of hereditary nonpolyposis colorectal cancer (HNPCC). WT5”HZMethods Ten kindreds of HNPCC in South Zhejiang area were analyzed. ResultsWT5”BZ Among the 10 families, 27 persons had colorectal cancer with age ranging from 19 to 71(average 45 3 years),74 percent of cases being diagnosed before the age of 50. The total of the colorectal cancer foci in 27 cases were 37, 40 percent of focis located proximal to splenic flexure, 30 percent of cases had multiple primary colorectal cancer, among which 14 8 percent of cases had synchronous multiple primary colorectal cancer, 18 5 percent of cases had metachronous multiple primary colorectal cancer. Thirteen cases died during follow-up of 1～23 years, 14 cases were still alive during follow-up of 1～28 years. KG2Conclusion HNPCC has definite charactistics that are of much help to early diagnosis.

Objective To investigate the effects of low dose of extracellular histone on endothelial cells in infectious diseases such as sepsis. Methods The endothelial cells were treated with 10 μg/mL recombinant human histone H3/H4 complex in replacement of calf thymus histones (CTH) for various periods of time, and the morphology changes and the viability of the endothelial cells were recorded. In addition, flow cytometry was applied to identify the characteristics of endothelial cells and enzyme-linked immunosorbnent assay (ELISA) was used to detect the extracellular histones level in endothelial cells culture. Results The low dose of CTH could continuously induce endothelial cells death, cell morphological changes and function loss, which was reproduced by 10 μg/mL recombinant histone H3/H4 complex. Results of histones quantitation showed that histone can cause a series of intracellular reactions in a short period of time. Conclusions It is showed that 10 μg/mL H3/H4 can induce the toxicity in infectious disease and this level of the dose is a lower than those used in previous studies and more close to the pathological conditions.

Objective To study Medpor combined with autogenous outer table of calvarial in management of deformities secondary to orbitozygomatic fracture.Methods In the reconstruction of the orbitozygomatic fracture,we firstly did the operation of osteotomies to reset reliable internal fixation,then Medpor combined with autogenous outer table of calvarial was used to repair wall and in correction of depressed orbitozygomatic region.Results From January 2007 to December 2010,13 cases of deformities secondary to orbitozygomatic fracture were repaired.The enlarged orbital volume was reduced,enophthalmos and facial asymmetry were corrected or improved.Facial deformity was significantly improved and no complications occurred.Conclusions Medpor combined with autogenous outer table of calvarial is benefit to most of secondary deformities to orbitozygomatic fracture.It is an effective solution to the orbital content shift and double vision problem,and it also can reduce the autologous damage and foreign body rejection.