ObjectiveTo investigate the clinical value ofDNA ploidy analysis in the diagnosis of benign and malignant pleural effusion.MethodsDNA ploidy in 24 benign pleural effusion and 39 malignant pleural effusion were detected by flow cytometry (FCM) and compared with the results of cytologic detection at the same time.ResultsThe positive rates of FCM detection in benign and malignant pleural effusion were 8.33％(2/24) and 64.10％ (25/39),there was significant difference (P＜0.05).The positive rates of cytologic detection in benign and malignant pleural effusion were 4.17％( 1/24 ) and 53.85％( 21/39),there was significant difference (P＜0.05).The sensitivity of FCM and cytologic detection in malignant pleural effusion was 64.10％ (25/39) and 53.85％ (21/39),the specificity of two methods was 91.67％ (22/24) and 95.83％ (23/24.),the results of two methods showed no significant differences (P ＞0.05).ConclusionDNA ploidy analysis by FCM has important clinical value in the diagnosis of benign and malignant pleural effusion.

Objective: To evaluate the diagnostic value of IL-6,IL-8,and TNF-? in malignant pleural effusion and tuberculous pleural effusion.Methods:We detected IL-6,IL-8,and TNF-? in malignant pleural effusion(21 cases)and tuberculous pleural effusion(13cases).Results:The mean values of the three cytokines measured in tuberculous pleural effusion were significantly higher than malignant pleural effusion( P

Objective:To implove the level of diagnosis and treatment of renal tuberculosis complicated with active pulmonary tuberculosis.MethodsThe diagnosis and treatment of 28 cases with renal tuberculosis complicated with active pulmonary tuberculosis were analyzed.Results:88.2% of patients were negative in PPD test, and 55.6% positive in the sputum acid fast stain. All the patients except 4 received thetreatment of anti-tuberculosis continuosly for 3-6 months before surgery.Conclusion:It is necessary to treat the patients with anti-tuberculosis drugs and improve their immune function before proper surgical treatment.Patients in fair general condition could be operated on 2 weeks after anti-tuberculosis therapy.

Objective To study the related factors of coronary no-reflow phenomenon in H-hypertensive acute myocardial infarction (AMI) patients after percutaneous coronary intervention (PCI).Methods 120 AMI patients were selected as the study subjects,all patients were given PCI,the infarct-related coronary artery stenosis forward flow(TIMI) grade ≦ 2 level was the no-flow criteria.The patients were divided into two groups:the no-reflow group (35 patients),the other 85 cases classified as normal blood group.The age,gender,body mass index (BMI),history of diabetes,hyperlipidemia hyperlipidemia history,family history,blood glucose,homocysteine (Hcy),white blood cell count,C-reactive protein (CRP),erythrocyte sedimentation rate (ESR),creatine kinase (CK),creatine kinase (CK-MB),triglycerides,total cholesterol,high density lipoprotein (HDL),low density lipoprotein (LDL),troponin-Ⅰ (cTn-Ⅰ),treatment time and other factors were observed and compared.Logistic regression analysis was used to identify relevant factors of no-reflow.Results 120 patients with primary PCI,no-reflow in 35 patients,the incidence rate was 30.1％.The sex,smoking history,history of hyperlipidemia,diabetes,history of angina,and Killip classification before treatment,time from chest pain to before treatment between no-reflow patients and normal blood flow patients had no significant differences (t =1.275,P ＞ 0.05).Multivariate Logistic regression analysis showed that:age,diabetes,triglycerides,HCY,cTn-Ⅰ,CK-MB,CRP were related factors of no-reflow(t =3.427,P ＜ 0.05).Conclusion Age,without pre-infarction history of angina,coronary artery open long hours,admission NYHA low,white blood cell count high,CK-MB and cTn-Ⅰ levels higher are independent risk factors of no-reflow phenomenon,PCI with no-reflow phenomenon indicates the poor prognosis.

Objective To investigate the effects of preoperative biliary drainage ( PBD ) on the morbidity and mortality of pancreaticoduodenectomy in patients with malignant obstructive jaundice in the lower bile duct. Methods Clinical data of 74 cases undergoing pancreaticoduodenectomy from Jan. 2000 to Dec. 2005 with preoperative total bilirubin level over 85 μmol/L were collected and retrospectively analyzed. Comparison was performed between patients receiving PBD and those not. Before surgery. The parameters sincluding perioperative situation, in-hospital death rate, post-operative complications and were calculated to evaluate the influence of pre-operative biliary drainage on the outcomes. Univariate analysis and Logistic analysis were used to identify the risk factors for post-operative complications. Results Forty (40/74) cases received PTCD procedure. The total bilirubin was significantly reduced from (338. 10±88. 38 )μwnol/L to ( 228. 50±82.24) μ,mol/L in PTCD population and was significantly lower than ( 328. 60±93. 02) μmol/L of the non-PTCD group. There was no significance between the two groups in terms of total complications and individual complication Logistic regression analysis showed that preoperative TB over 340 μmol/L and blood loss over 600 ml were important risk factors for post-operative complications. Conclusion High pre-operative total bilirubin over 340 μmol/L increases the risk of post-operative complications in the patients with malignant jaundice. Preoperative biliary drainage is useful to reduce the total bilirubin and improve the hepatic function of the patients. Prolonged pre-operative biliary drainage could be considered in the patients with high preoperative bilirubin,poor nutritional condition and impaired coagulation to enhance the tolerance of surgery.

A method was developed for the determination of 39 polybrominated diphenyl ethers(PBDEs) from mono- to hepta-brominated in coastal sediment by gas chromatography/mass spectrometry. The sample was extracted with a solution of dichloromethane: n-hexane( 1:1,V/V) ,followed by ultrasonic extraction for 60 min at 25℃. The clean-up step was optimized using silica gel and alumina. The detection limit of the method was in the range of 0.003 - 0.10μg/kg. The results indicate that the average recovery of the internal standard was from 66.2% to 118. 6%. The relative standard deviation RSD( % ) was from 0. 8 % to 18. 2%. The method for the detection of 39 PBDE congeners in coastal sediment was sensitive,high reproducible with satisfactory recoveries.

Faced with huge demand for building public health capacity in the post-Ebola era, Africa Union has planned to establish Africa Center for Disease Control and Prevention ( Africa CDC,ACDC), with headquarters in Addis Ababa, Ethiopia, and at least 5 regional collaborating centers in each region of the continent .The strategic objectives of ACDC focus on building a continental integrated public health system and strengthening needed public health capacity .The current study analyzed the background , plans, development layout and strategy of ACDC , and provided references for China-Africa cooperation in the areas of public health and for the formulation of China global strategies of biosecurity and disease prevention and control .

Objective To study the application of the intelligent optimum tube voltage ( Care kV) combined with automatic tube current ( Care Dose 4D) technique in dual source CT scanning for the abdomen .Methods 180 adult patients underwent whole abdominal CT examination from February 2016 to March 2016 of were selected as the retrospective research objects .On the basis of reference mAs of the image quality the patients were divided into three groups using completely randomized grouping method with 60 patients in each group, such as 250 mAs group, 200 mAs group, and 150 mAs group.The other scanning parameters and image reconstruction parameters were the same .The liver parenchyma CT values and image noise( SD) at the second hepatic portal level were measured .The signal-to-noise ratios ( SNR) in the three groups were compared as well as contrast to noise ratio ( CNR ) , noise ( SD ) , the average volume CT dose index (CTDIvol), dose length product (DLP) and effective dose(E).Results The kV and mAs values in the plain scan , arterial phase scan and portal phase scan in the three groups of patients had statistically significant differences(F=35.25, P<0.05).With the reducing of the refference mAs, the frequency of choosing lower kV in three groups of patients increased gradually .The CTDIvol, DLP and E of plain scan, arterial and portal phase in three groups of patientshad significant difference (F=31.51, P<0.05).With the decline of the reference mAs, the radiation dose decreased gradually , with 150 mAs group lowest.The SNR, CNR and noise of three phases in 250 mAs group and 200 mAs group had no statistically significant difference (F=1.61, P>0.05).In 250 mAs group and 150 mAs group, the SNR and the noise of plain scan and arterial phase images had no statistically significant difference ( F=1.98, P>0.05) , and SNR of portal phase images and CNR of three phases had statistically significant difference (F=27.64, P<0.05);In group 200 mAs and group 150 mAs, the SNR, CNR and noise of plain scan and arterial phase images had no statistically significant difference (F=1.95, P>0.05), and the SNR and CNR of portal phase images had significant difference (F=19.63, P<0.05).Conclusions For the combined use of Care kV and Care dose 4D,it is feasible to select appropriate reference mAs in reducing radiation dose without sacrificing imaging quality .

0.05) none of the 39 cases of small cell type was in the AB blood group

Objective To analyze the clinic and imaging characteristics as well as development of intervertebral disc calcification in children.Methods 6 cases underwent intervertebral disc calcification.X-ray films and CT scan of cervical spine had been taken in all cases,two cases had MRI examination.All cases were followed-up for 2 to 3 years by radiograph and CT.Results Only one intervertebral disc involved in all cases.X-ray and CT findings included disc calcification,disc herniation and change of vertebral boy in form.The clinical symptoms disappeared after conservative therapy 2 to 4 weeks.But the disc calcification disappeared until 6 months to 3 years.Conclusion Intervertebral disc calcification in children is a self-limiting disease with excellent prognosis,its diagnosis depends on imageology.