EGFR/HER-2 is an attractive therapeutic target for solid tumors.Lapatinib is an orally administered dual inhibitor of EGFR/HER-2 tyosine kinases.Preclinical experiments in vitro and in vivo models indicate that lapatinib is active against various solid tumors.Phase Ⅰ trials have shown an acceptable adverse event.Phase Ⅱand Ⅲ trials demonstrate the promising results for the treatment of metastatic,refractory,inflammatory,or brain metastatic breast cancer.With the further developments of biology,the molecular targeted chemotherapy becomes a novel adjuvant therapy for advanced breast cancer patients.

Systemic lupus erythematosus(SLE)is an autoimmune disease that affects primarily women,commonly in their reproductive years but does not influence fertility.More recent prospective studies indicate that pregnancy is safe for the majority of mothers if it is planned when SLE is quiescent.In pregnant women with lupus nephritis,the outcome of the fetal and maternal is strongly correlated with lupus activity,kidney function and the presence of aPL at the time of conception.To prevent renal complications,prednisone is given after conception,and reinforcement for a few days before the estimated delivery and for a week after delivery or miscarriage with rapid tapering to maintenance levels may be suggested.Treatment of flares includes corticosteroids,hydroxychloroquine,azothioprine and cyclosporin A.Blood pressure is controlled with methyldopa,labetalol nifedipine or hydralazine.

0.05). Platelet decreased significantly in group A (P

China has high prevalence of gastric cancer, with the morbidity and mortality leading the list of malignancy. The conditions of diagnosis and treatment of gastric cancer in China fail to meet the need of a large number of patients because of restriction in re-gional development. Therefore, we are facing a very serious situation of fighting against gastric cancer. Multidiscipline, individualiza-tion, and standardization are the development tendency of diagnosis and treatment of gastric cancer. Numerous topics about gastric cancer are currently controversial and solutions to these problems depend on the development of evidence-based medicine. This re-view summarized the recent progress in the clinical methods used for stomach cancer, laparoscopic surgical techniques, diagnosis and treatment of early gastric cancer, conversion therapy of advanced gastric cancer, therapeutic strategy of esophageal-gastric junction cancer, enhanced recovery after surgery, and translational research and clinical trials of gastric cancer. Moreover, the foci and prob-lems of the diagnosis and treatment of gastric cancer were discussed to provide reference for further studies.

Objective To investigate the effects of minocycline on regional cerebral blood flow and the expression of endothe-lin-1(ET-1) in ischemic penumbra of rats after focal cerebral ischemia reperfusion injury .Methods Middle cerebral artery occlusion (MCAO) with nylon suture was used to be established as focal cerebral ischemia reperfusion mode (I/R) ,a total of 35 male Spra-geue-Dawley rats were randomly divided into 3 groups :sham-operated group(n= 10) ,model group(n= 15) and minocycline group (n= 15) .After 24 hours of I/R ,The neurobehavioral function of rats was evaluated by Longa′s test ,the regional cerebral blood flow in ischemic penumbra was assessed with laser-Doppler flowmetry .After 6 and 24 hours of I/R ,the expression of ET-1 in peri-in-farct region was measured by both immunohistochemistry and radioimmunoassay .Results Compared with sham-group ,the Longa′s test scales ,ET-1 protein expression increased and the rCBF decreased in ischemic penumbra in model group(P< 0 .05) .the Longa′s test scales ,ET-1 protein expression decreased and the rCBF decreased in ischemic penumbra in minocycline group when compared to the model group(P< 0 .05) .Conclusion Minocycline could promote neurological functional recovery of rats after MCAO ,which might be attributed to increase the cerebral blood flow and regulate the endothelin-1 expression in ischemic penumbra .

AIM: Theaflavins are the major constituent of black tea, and have a lot of pharmacological activities, for example, regulating blood fat, anti-oxygen, anti-tumor, resisting cerebrovascular disease, and so on. Previously, theaflavins' effect on human fat metabolism mostly belongs to macro-analysis, but this experiment try to find out the effect of theaflavins on the differentiation of rabbit mesenchymal stem cells (MSCs) into adipocytes in terms of cells. METHODS: The experiment was completed in the center laboratory of Anhui Provincial Hospital Affiliated to Anhui Medical University from May to September in 2007.①Ten rabbits of cleaning grade and 4-8 weeks old were provided by the animal experiment center of Anhui Medical University. The disposal of animals in process of experiment referred to the ethical standard of animals.②The heparinized rabbits were sacrificed in drugged state, then bilateral femur, tibia and humerus were obtained and their soft tissues were removed, cutting epiphysis of two sides including epiphyseal plate. The MSCs were isolated and cultured with whole bone marrow culture method, inoculated at a density of 2?108 L-1. When the cells grew to the fusion of 80%-90%, digesting and passage culture were performed. Cells of the third generation were collected and inoculated at a density of 1?105/cm2 to be cultured for 2 weeks with DMEM adipocyte-induced liquid, which contained recombinant human insulin 10 mg/L, dexamethasone 10-6 mol/L, and IBMX 0.5 mmol/L. Three groups were set up: adipocytes control group in 1 mL adipocyte-induced liquid; theaflavins group with the 500 ?g/L theaflavins in 1 mL adipocyte-induced liquid; blank group in equal amount of ordinary DMEM culture medium.③The cultured cells of the second generation were selected to draw growth curve; oil O staining was used to identify the induced adipocytes and calculate the differentiation efficiency of adipocytes. RESULTS: ①Cell growth curve: MSCs had vigorous reproductive activity, 1 day of the culture was the cell adaptive phase, 3 days was the increased logarithmic phase, and 8 days was the platform phase. After 8 days, cell proliferation quickly stepped down and cells reduced.②Identification of adipocytes by oil O stain: Lipid droplets in adipocytes were stained into salmon pink, and the nucleus was stained into blue. Most of MSCs in adipocyte control group were induced into adipocytes, the differentiation efficiency was (64.8?4.8)%, while that in theaflavins group was only (32.0?3.4)%. No adipocytes obviously formed in blank control group. CONCLUSION: Theaflavins can obviously inhibit differentiation from rabbit MSCs to adipocytes.

Objective To explore the application of near infrared fluorescent dye C-dots in cell labeling.Methods B16 melanoma cells were cultured with C-dots of various diameters.The distribution of C-dots in cells was observed by laser confocal fluorescent microscope.The proliferation of both C-dots-labeled and unlabeled cells were measured by MTT colorimetry.The viability of labeled cells was checked by trypan blue staining.Results C-dots with diameter of 5 or 10 nanometers can be used to label cells.The near infrared fluorescence of C-dots was taken into the cells and observed on the cellular membrane.No difference of cellular viability was found between the labeled and unlabeled cells.Conclusion C-dots can be used as an intracellular marker for the research on trace and proliferation of cells.

Objective To establish the HPLC fingerprints of Fructus Ligustri Lucidi.Methods HPLC-ELSD analysis was carried out with Lichrospher C18 column(4.6 mm ? 250 mm,5 ?m).The detector was Alltech ELSD 2000.The method was developed by gradient elution with methanol and water as the mobile phase.With oleanolic acid as the marker,sixteen batches of Fructus Ligustri Lucidi were analyzed with computer-aided similarity evaluation system.Results HPLC fingerprints of Fructus Ligustri Lucidi showed 14 characteristic peaks and the similarity of the fingerprints of 10 batches of samples was over 0.90,indicating the fingerprints being stable and repeatable.Conclusion The method is simple,accurate and with a good reproducibility,and can be used for the identification and quality control of Fructus Ligustri Lucidi.