Objective To explore the influence factors of thrombocytopenia in patients with severe infection in ICU ,so as to provide a reference for clinic prevention and treatment and the judgment of prognosis .Methods 76 patients(39 male ,37 female) with severe infection from May 2014 to December 2014 were retrospectively analyzed in this hospital .Among them there were 40 cases with thrombocytopenia (Group A ) ,and 36 patients without thrombocytopenia (group B) .Influence factors and prognosis differences were investigated for thrombocytopenia combine with severe infection .Results There was correlation between the age , numeration of leukocyte ,neutrophil ratio ,gram negative bacteria infection ,respiratory infection and severe infection combine with thrombocytopenia(P < 0 .05 ) ,ICU dwell time and mortality differences between the two groups was statistic significance ( P<0 .05) .Conclusion Platelet count should be monitored in patients with severe infection ,especially in those with old age ,respiratory infections ,Gram negative bacteria infection ,we should give priority intervention and find the point in time in order to prevention and cure and improve its poor prognosis .

Aim To determine the effect of phospholipase C(PLC) on the rabbit platelet triphosphoryl inositol(IP_3) induced by ADP,and then to explore the mechanism of anti-aggregation to platelet by PLC.Method PRP was made from rabbit blood by removing red cells,and then was parted to eight groups.The first group was treated with PSS.The second was treated with with ADP.The third was treated with ASP and then induced by ADP.Four to eight groups were treated with different dose of PLC respectively and then induced by ADP.IP_3 of rabbit platelet was made by trichloro-acetic acid method,and its content was tested by radioimmunological assay.Result IP_3 of rabbit platelet treated with PSS was 0.29 pmol/10~8 platelet.IP_3 of rabbit platelet treated with ADP was 0.39 pmol/10~8 platelet.IP_3 of rabbit platelet,which were treated with ASP 668 ?mol?L~(-1),5,10,15,20 and 25 U PLC?ml~(-1) respectively and then induced by ADP,were 0.29,0.19,0.08,0.15,0.25,0.04 and 0.18 pmoL/10~8 platelet respectively.IP_3 of ADP group was apparently higher than that of PSS group,but the IP_3 of ASP group and different dose PLC group were significantly lower than ADP group(P

Objective:To investigate the expression of the collagenases MMP-13 and its inhibitor, tissue inhibitor metalloproteinase-1 (TIMP-1) in the rat lung of the experimental models of COPD.Methods:Male Wistar rats (10 weeks of age) were divided into two groups—model and control groups.The rat of model group were given intratracheal lipopolysaccharide (LPS),and then animals were exposed to cigarette smoke for 32 days.The lung function was measured,and pathological changes were also observed.Transcriptional levels of MMP-13 and TIMP-1 mRNA extracted from the lungs were assessed by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR).Results:(1)In COPD model group,the pathological changes of bronchi and lung tissue,the changes of lung function were similar to those of the COPD patients.(2)The mRNA expression of MMP-13 and TIMP-1 in COPD model group were significantly increased compared with those in control group( P

Objective To investigate the ultrasonographic features corresponding to classification of peripheral neuropathies in neurofibromatosis type Ⅰ.Methods Thirty-six patients with peripheral neuropathies and diagnosis of neurofibromatosis type I were enrolled in this study.Ultrasound appearance of peripheral neuropathies was analyzed and divided into four groups according to their pathological classifications.There were 19 males and 17 females with age between 7-47 years.High frequency or board frequency ultrasound probes were used for scanning.Results The ultrasound findings were varied based on different pathological classifications.Multiple nodular class(n=5) was presented as multiple nodes.Ultrasound demonstrated that tumors were multiple,oval or lobulated,and had well defined margin,and the echogenic of lesions was mainly hypoechoic.Plexiform class (n=12) was presented as cluster nodes along the peripheral nerve trunk.Ultrasound showed trunk nerves in extremities became thicker in a long range and lost the normal structure,and catenular or racemosum in shape.The echogenicity was hypoechoic.Diffuse class (n=14) was diffuse subcutaneous disease.Tumors range was infiltrative.The subcutaneous soft tissue was thicken and echogenicity was increased.The structure of nerve bundle was disturbed and associated with multiple hypoechoic nodes.Mixture class (n=5) was defined as mixture of multiple nodular class and plexiform class.In this group,diffuse disorders were co-existed with multiple nodes disorder.Conclusions The ultrasonographic appearances of peripheral nerve disorders in neurofibromatosis type I was closely corresponded to their pathological characters.And the ultrasonic examination would be helpful to diagnosis and provide important information for therapy.

Positive psychology focuses on enhancing individual positive emotion and promoting the development of individual positive personality.Introducing positive psychology into the first stage of internal medical resident standardized training will improve the resident ability to solve clinical problems.It's helpful for resident doctors to improve their clinical thinking ability,doctor education innovation ability and lifelong self-education ability.It inspires their creative thinking by motivating their positive emotions It would help them to avoid occupational tiredness.Using the idea of positive psychology could improve the comprehensive quality of residents.

This paper summarized the effects of the treatment of chronic hepatitis B (CHB) by lamivudine combined with traditional Chinese medicine or western medicine in the past few years. Combined treatment of lamivudine and the other medicine had higher efficiency than lamvudine alone in the treatment of CHB. The Combined treatment should be a tendency of the treatment for chronic hepatitis B. The focal point is that the traditional Chinese recipe produces a multitarget effect in the treatment of CHB. The combined treatment of lamivudine and traditional Chinese recipe can reduce the treatment course and the recurrence rate. Combined treatment of traditional Chinese medicine and western medicine has notable superiority and broad developing prospect in the treatment of CHB.

Amino acids (AA) as substrates of gluconeogenesis could promote endogenous glucose production.Some AA can enhance insulin secretion. Recently,many studies have found that some AA can also activate mammalian target of rapamycin-S6 kinase 1 ( mTOR-S6K1 ) pathway,resulting in maintaining pancreatic β cell function on one hand,and on the other hand,inhibiting insulin signal transduction of insulin-sensitive cells and thus causing insulin resistance.

Objective To investigate the significance of detecting the level of CRP,TNF-α,IL-6 and IGF-1 in the patients with type 2 diabetes mellitus(T2DM).Methods Sixty patients with T2DM in the Pingliang Municipal People′s Hospital from February 2013 to March 2015 were selected as the observation group And contemporaneous 60 individuals undergoing healthy physical exami-nation were selected as the control group.The levels of FBG,2 hBG,FINS,HbA1c,CRP,TNF-α,IL-6 and IGF-1 were detected in these two groups.The correlation between CRP,TNF-α,IL-6 and IGF-1 with FBG,FINS and HbA1c were analyzed.Results The levels of FBG,2 hBG and HbA1c in the observation group were significantly higher than those in the control group,the difference was statistically significant (P<0.05).The FINS level in the observation group was significantly lower than that in the control group,the difference has statistically significant (P<0.05).The levels of CRP,TNF-α,IL-6 and IGF-1 in the observation group were (8.46±2.45)mg/L,(18.56±4.23)ng/L,(13.53±3.24)pg/L and (125.33±23.14)ng/L respectively,which were signifi-cantly higher than (3.23±1.02)mg/L,(11.25±3.01)ng/L,(7.24±1.78)pg/L and (98.45±18.56)ng/L in the control group, the difference was statistically significant(P<0.05).CRP,TNF-αand IL-6 were positively correlated with FBG,FINS and HbA1c;IGF-1 was negatively correlated with FBG and HbA1c.Conclusion Detecting the CRP,TNF-α,IL-6 and IGF-1 levels can provide a basis for the diagnosis of T2DM and thinking for its treatment.

A method was developed for the determination of 39 polybrominated diphenyl ethers(PBDEs) from mono- to hepta-brominated in coastal sediment by gas chromatography/mass spectrometry. The sample was extracted with a solution of dichloromethane: n-hexane( 1:1,V/V) ,followed by ultrasonic extraction for 60 min at 25℃. The clean-up step was optimized using silica gel and alumina. The detection limit of the method was in the range of 0.003 - 0.10μg/kg. The results indicate that the average recovery of the internal standard was from 66.2% to 118. 6%. The relative standard deviation RSD( % ) was from 0. 8 % to 18. 2%. The method for the detection of 39 PBDE congeners in coastal sediment was sensitive,high reproducible with satisfactory recoveries.

Neural stem cells are a study hot spot in neurobiological science and neurosurgery in the near future. The transplantation of neural stem cells already became a new way which be used for treating the injury and degenerative disease in the nervous system, because of the self-renewal and cell differentiation bionomics. However, how to get massive neural stem cells, which can be used in animal experiment and clinical application, has constrainted the development of stem cell technique.This article introduced the harvesting methods of embryonic stem cells, adult neural stem cells and mesenchymal stem cells, in order to lead cellular transplant treatment into clinical treatment plan by a safe and effective mode.