Objective:To investigate the expression of the collagenases MMP-13 and its inhibitor, tissue inhibitor metalloproteinase-1 (TIMP-1) in the rat lung of the experimental models of COPD.Methods:Male Wistar rats (10 weeks of age) were divided into two groups—model and control groups.The rat of model group were given intratracheal lipopolysaccharide (LPS),and then animals were exposed to cigarette smoke for 32 days.The lung function was measured,and pathological changes were also observed.Transcriptional levels of MMP-13 and TIMP-1 mRNA extracted from the lungs were assessed by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR).Results:(1)In COPD model group,the pathological changes of bronchi and lung tissue,the changes of lung function were similar to those of the COPD patients.(2)The mRNA expression of MMP-13 and TIMP-1 in COPD model group were significantly increased compared with those in control group( P

Objective To explore the influence factors of thrombocytopenia in patients with severe infection in ICU ,so as to provide a reference for clinic prevention and treatment and the judgment of prognosis .Methods 76 patients(39 male ,37 female) with severe infection from May 2014 to December 2014 were retrospectively analyzed in this hospital .Among them there were 40 cases with thrombocytopenia (Group A ) ,and 36 patients without thrombocytopenia (group B) .Influence factors and prognosis differences were investigated for thrombocytopenia combine with severe infection .Results There was correlation between the age , numeration of leukocyte ,neutrophil ratio ,gram negative bacteria infection ,respiratory infection and severe infection combine with thrombocytopenia(P < 0 .05 ) ,ICU dwell time and mortality differences between the two groups was statistic significance ( P<0 .05) .Conclusion Platelet count should be monitored in patients with severe infection ,especially in those with old age ,respiratory infections ,Gram negative bacteria infection ,we should give priority intervention and find the point in time in order to prevention and cure and improve its poor prognosis .

Aim To determine the effect of phospholipase C(PLC) on the rabbit platelet triphosphoryl inositol(IP_3) induced by ADP,and then to explore the mechanism of anti-aggregation to platelet by PLC.Method PRP was made from rabbit blood by removing red cells,and then was parted to eight groups.The first group was treated with PSS.The second was treated with with ADP.The third was treated with ASP and then induced by ADP.Four to eight groups were treated with different dose of PLC respectively and then induced by ADP.IP_3 of rabbit platelet was made by trichloro-acetic acid method,and its content was tested by radioimmunological assay.Result IP_3 of rabbit platelet treated with PSS was 0.29 pmol/10~8 platelet.IP_3 of rabbit platelet treated with ADP was 0.39 pmol/10~8 platelet.IP_3 of rabbit platelet,which were treated with ASP 668 ?mol?L~(-1),5,10,15,20 and 25 U PLC?ml~(-1) respectively and then induced by ADP,were 0.29,0.19,0.08,0.15,0.25,0.04 and 0.18 pmoL/10~8 platelet respectively.IP_3 of ADP group was apparently higher than that of PSS group,but the IP_3 of ASP group and different dose PLC group were significantly lower than ADP group(P

Objective To investigate the ultrasonographic features corresponding to classification of peripheral neuropathies in neurofibromatosis type Ⅰ.Methods Thirty-six patients with peripheral neuropathies and diagnosis of neurofibromatosis type I were enrolled in this study.Ultrasound appearance of peripheral neuropathies was analyzed and divided into four groups according to their pathological classifications.There were 19 males and 17 females with age between 7-47 years.High frequency or board frequency ultrasound probes were used for scanning.Results The ultrasound findings were varied based on different pathological classifications.Multiple nodular class(n=5) was presented as multiple nodes.Ultrasound demonstrated that tumors were multiple,oval or lobulated,and had well defined margin,and the echogenic of lesions was mainly hypoechoic.Plexiform class (n=12) was presented as cluster nodes along the peripheral nerve trunk.Ultrasound showed trunk nerves in extremities became thicker in a long range and lost the normal structure,and catenular or racemosum in shape.The echogenicity was hypoechoic.Diffuse class (n=14) was diffuse subcutaneous disease.Tumors range was infiltrative.The subcutaneous soft tissue was thicken and echogenicity was increased.The structure of nerve bundle was disturbed and associated with multiple hypoechoic nodes.Mixture class (n=5) was defined as mixture of multiple nodular class and plexiform class.In this group,diffuse disorders were co-existed with multiple nodes disorder.Conclusions The ultrasonographic appearances of peripheral nerve disorders in neurofibromatosis type I was closely corresponded to their pathological characters.And the ultrasonic examination would be helpful to diagnosis and provide important information for therapy.

Positive psychology focuses on enhancing individual positive emotion and promoting the development of individual positive personality.Introducing positive psychology into the first stage of internal medical resident standardized training will improve the resident ability to solve clinical problems.It's helpful for resident doctors to improve their clinical thinking ability,doctor education innovation ability and lifelong self-education ability.It inspires their creative thinking by motivating their positive emotions It would help them to avoid occupational tiredness.Using the idea of positive psychology could improve the comprehensive quality of residents.

This paper summarized the effects of the treatment of chronic hepatitis B (CHB) by lamivudine combined with traditional Chinese medicine or western medicine in the past few years. Combined treatment of lamivudine and the other medicine had higher efficiency than lamvudine alone in the treatment of CHB. The Combined treatment should be a tendency of the treatment for chronic hepatitis B. The focal point is that the traditional Chinese recipe produces a multitarget effect in the treatment of CHB. The combined treatment of lamivudine and traditional Chinese recipe can reduce the treatment course and the recurrence rate. Combined treatment of traditional Chinese medicine and western medicine has notable superiority and broad developing prospect in the treatment of CHB.

To increase patint anesthesia safety, a device for anesthesia respiratory circle and montoring line fixation is designed. The device is composed of the railroad, cardan shaft, union join and fixation plate. Being simple, flexible, reliable and easy to use and store, it has a brilliant perspective in clinical application.

Objective To investigate whether the serum adiponectin is related to the atherosclerosis in type 2 diabetes.Methods Thirty three normal control subjects(NC group),66 type 2 diabetes subjects without atherosclerosis(NAS group),and 66 type 2 diabetes subjects with atherosclerosis(AS group) were recruited.The intima media thickness(IMT) of dorsalis pedis artery were measured using B-mode Doppler ultrasonography.Serum adiponectin and other gluco-lipid metabolic indices were determined.Results 1)Compared with NC group and NAS group,the IMT value in AS patients were significantly increased(P

Objective To study the serum levels of PSA, PAP joint detection in diagnosis of prostate cancer and the effect of estrogen on it.Methods60 patients with prostate cancer were selected in Jinyun County People's Hospital department of urology patients, giving diethylstilbestrol 3mg, oral once a day, and give Aspirin Enteric-coated Sustained Release Tablets 100mg oral, once daily, for 2 consecutive months.At the same time, we selected 60 cases of patients with benign prostatic hyperplasia, normal control group of 60 healthy subjects were the same period.Using radioimmunoassay, were observed inprostatic hyperplasia group, normal control group, the prostate cancer group before treatment, the prostate cancer group after treatment, serum PSA, PAP levels, as well as the group before and after treatment in patients with prostate cancer IPSS score index.ResultsThe prostate cancer group, serum PSA, PAP levels were significantly higher than that of benign prostatic hyperplasia group and normal control group, the difference was statistically significant (P<0.05);no significant difference was found between the prostatic hyperplasia group and normal control group, PSA in prostate cancer positive detection rate and the detection sensitivity is 78.33% the specificity was 70%, the index of PAP in prostate cancer, and the positive detection rate and the detection sensitivity was 53.33%, specificity index reached 93.33%;PSA for prostate cancer detection positive rate and sensitivity was higher than that of PAP, but the specificity is less than PAP, the difference is statistically significant (P<0.05) after treatment;the prostate cancer group, serum PSA, PAP levels were improved, compared with that before treatment, serum PSA, PAP levels decreased significantly, the difference was statistically significant (P<0.05);and after the treatment, the prostate cancer group of prostate symptoms have improved, compared with before treatment, IPSS score decreased significantly, the difference was statistically significant (P<0.05).ConclusionThe combined detection of serum PSA, PAP in the diagnosis of prostate cancer can improve the sensitivity and specificity, and can be used for early diagnosis of prostate cancer;estrogen can lower their level, improve the symptoms of prostate cancer, inhibit the progress of the disease.

Objective To compare the analytical performance and results correlation of cortisol detection by the Beckman Access 2 Immunoassay System chemiluminescence system and the Roche COBAS E 411 electrochemical luminescence system .Methods The precision ,functional sensitivity ,reference range ,positive coincidence rate and negative coincidence rate of both Beckman Access 2 Immunoassay System and Roche COBAS E 411 systems for detecting cortisol were evaluated according to the method of ISO15198 .Results Both Beckman Access 2 Immunoassay System and Roche COBAS E 411 system exhibited better precision ,the functional sensitivity conformed to the lower limit of clinical detection set by the manufacturers ,after establishing the reference range of cortisol by the laboratory ,the cortisol values detected by these two systems were performed the linear regression analysis , the results indicated that the correlation between them was good ,the linear regression coefficient r2 =0 .977 ,and the positive and negative coincidence rates were 100% .Conclusion The two analytical systems have excellent performance for cortisol ,which all meet the clinical detection requirements .The methodological comparison shows good correlation between them ,and the detection re‐sults could be cited between these two systems by the regression equation .