0.05).The AOD of ?2 subunit protein immunoreactivity in the frontal lobe and CA4 region decreased significantly in the seizure group than those in the control group(P0.05),but the expressions of ?1 subunits mRNA in the hippocampus and ?2 subunit mRNA in the cerebral cortex in the seizure group decreased significantly as compared with those in the control group(P

Faced with huge demand for building public health capacity in the post-Ebola era, Africa Union has planned to establish Africa Center for Disease Control and Prevention ( Africa CDC,ACDC), with headquarters in Addis Ababa, Ethiopia, and at least 5 regional collaborating centers in each region of the continent .The strategic objectives of ACDC focus on building a continental integrated public health system and strengthening needed public health capacity .The current study analyzed the background , plans, development layout and strategy of ACDC , and provided references for China-Africa cooperation in the areas of public health and for the formulation of China global strategies of biosecurity and disease prevention and control .

Objective To explore the effects of sports training on endothelin-1 ( ET-1 ) and endothelin-1 receptor (ETR) and its subtypes in the myocardium in rats. Methods After sports training, levels of endothelin-1 were measured using radioimmunoassay, and changes in ETAR and ETBR levels by radioligand binding assay. Results The endothelin-1 level in myocardial cells was significantly reduced ( P

Objective To investigate the clinical treatment principles of asymptomatic cholecystolithiasis. Methods 136 cases of asymptomatic cholecystolithiasis, found on health check up, were randomly divided into two groups :(1) Preventive cholecystectomy group,66 cases;(2)therapeutic cholecystectomy group,70 cases. The data of postoperative pathologic examination, complications and degree of operative difficulty between the two groups were compared. Results The postoperative pathologic examination, complications and degree of operative difficulty between the two groups were significantly different(P

Hip-stressed lower limb specific strength training apparatus is a new kind of training equipment with the combination of specific technical strength training and quantitative evaluating. Base on the mechanism of sports physiology and sports biomechanics, by applying the theory of motor learning and control, the apparatus adopts the training model of the "hip stress loading device" and "hip-feet starting and ending stress point" to simulate the track of working limb in running, jumping and throwing, controls the degrees of body movement freedom reasonably. For the first time, actualizes the heavy load specific strength and sports skill training of leg driving, leg pawing and trunk-backward knees-bend leg driving. The distinctive training pattern of forced track control and the training method of strength & skill coupling" used in this apparatus, makes the action in strength training approach to real sport skill in greatest extent, and shifts the result of strength training to the skill training as much as possible. So it remarkably increase the efficiency of strength training and has an great importance in changing the concept of strength training.

OBJECTIVE To establish hospital infection information surveillance system connecting with hospital information system(HIS)in order to achieve real time monitoring,process management and data upload for hospital infection.METHODS According to the national rules of law and the experience of hospital infection management,the software had been developed by using PowerBuilder as program tool and Oracle as database.RESULTS The software could be utilized in the real time monitoring for all inpatients.It had the function of inquiry,statistics and upload.CONCLUSIONS The subsystem connecting with HIS may improve efficiency and effectiveness for preventing and controlling hospital infection.

Objective To investigate and analyze structure of risk perceived by people using HIV voluntary counseling and testing services and related factors.Method Two hundred and sixty eight VCT users were selected from 2 CDCs and 2 hospitals in Beijing and their perceived risks in the process of VCT were assessed by using self-administered question- naires.Factor analysis was performed to understand the relationship between different perceived risks,and risks perceived by different users were evaluated.Results Six factors were obtained from the factor analysis which included:fear of priva- cy disclosure,embarrassment,concern over the reliability of HIV tests,cost & time consumption,the tragic outcomes of HIV positive tests,and potential medical expenditures in the future.And the risks perceived by low and high income users were much higher than those perceived by middle income users.Conclusion There are clear structure of risks perceived by people using HIV voluntary counseling and testing,and people with different incomes have different levels of perceived risks.

Objective To observe the marker effects of two different fluorescent dyes, DIL and DAPI, in labeling endogenous neural stem cells (ENSCs) in rat central nervous system. Methods Thirty-six Sprague-Dawley rats were randomized into staining groups, comprising DIL group and DAPI group, and the corresponding control groups, including DMSO group for DIL group and PBS group for DAPI group. 0.2% DIL 10μl or 10μg/ml DAPI 10μl was stereotactically injected into the lateral ventricle of rats of DIL group or DAPI group, while DMSO or PBS 10μl was introduced into that of DMSO group or PBS group. Neurological severity score (NSS) was determined 2 hours and 24 hours respectively after the operation. Rats were sacrificed at day 1, 3, 7 after the injection. Serial coronal sections of the brain and spinal cord were carried out on a cryostat, and then they were observed under a confocal microscope. The fluorescence intensity of the targeted area, which highlighted by labeled ependymal cells, in the brain and spinal cord of cervical vertebrae, thoracic vertebrae and lumbar vertebrae were semi-quantified. Fluorescence intensity of each section was measured in triplicate, and a mean value was obtained. Statistical analysis was performed on 3 data sets, randomly selected from sections of brain and spinal cord obtained at day 1, 3, 7. Results Two hours after DIL injection, the rats showed no evident neurological defect. NSS value was very low, and there was no significant difference compared with the DMSO group (P>0.05). Twenty-four hours later, normal neurological function recovered in all the rats. Red fluorescence could be seen in the cytoplasm of ependymal cells in the lateral ventricle and each spinal cord segment at day 1 after the DIL injection, and it did not disappear until the 7th day. Nuclei of DAPI-labeled lateral ventricle cells were blue, with clear nuclear morphology. Choroid plexus cells of the ventricle were also labeled. However, there was no blue fluorescence in the medulla oblongata or any segment ofthe spinal cord. The picture at day 3 and day 7 was similar to that of day 1. No significant difference was found between fluorescence intensity in DIL or DAPI stained cells(P>0.05) at any time point. Conclusions DIL may serve as a marker of the cytoplasm of ependymal cells in the brain ventricle and spinal central canal. DAPI, which is often used in the nuclear staining, can label the ENSCs in the brain ventricle. Intraventricular injection of fluorescent dye is a relatively safety procedure.

OBJECTIVE: To compare the efficacies of different target concentrations of propofol combinded with remifentanil for painless induced abortions, thus to explore the best target concentrations. METHODS: One hundred and twenty ASAI-II patients receiving elective painless induced abortions between May 2011 and October 2011 were randomly divided into three groups. The target concentrations of propofol were 2, 3, and 4 μg·mL-1, respectively. The hemodynamic changes and body movements at different time and the time of leaving operating room in the three groups were compared. RESULTS: The patients in the three groups all finished operations. The MAPs and HRs when dilating uterine and aspirating embryo bud were significantly increased, and the incidence of body movements was the highest in the 2 μg·mL-1 group; the number of apnea cases and the time of leaving operation room in the 4 μg·mL-1 group were significantly higher than the other two groups. CONCLUSION: When using propofol at target concentration of 3 μg·mL-1 combined with remifentanil at target concentration of 3 μg·mL-1 for painless induced abortions, the patients had stable perioperative MAP and HR, less apnea cases, and shorter time of leaving operation room. So these are the ideal target plasma concentrations for controlled anesthesia for painless induced abortions.

Objective To investigate clinical characteristics of the multi-center breast DCIS (ductal carcinoma in situ), in order to improve the rate of early diagnosis. Methods Clinical data of 23 multi-center breast DCIS patients admitted and operated on from September 2003 to February 2009 at our department were retrospectively reviewed. Results There were 4 cases associated with nipple discharge,and 18 cases with preoperative negative physical examination (78%). In the preoperative examination within 6 months, the positive rate of mammography was 90% mainly characterized by the existence of diffuse and multiple small calcifications. The positive rate of MRI was 25%, B ultrasound had no positive findings. The patients that were confirmed multi-center breast DCIS by intraoperative frozen section examination received mastectomy and ipsilateral axillary L1, L2 lymph node dissection. Conclusion The diffuse and multiple small calcification is one of early clinical characteristics of multi-center breast DCIS. Although the disease has a wide range of lesion, it still is an early tumor, and has a favourite prognosis after comprehensive treatment.