Objective:To investigate whether celecoxib(CXB), a specific COX-2 inhibitor,can inhibit the proliferation of cyst lining epithelial cells through blocking mitogen-activated protein kinase(MAPK) signal transduction pathway.Methods: Primarily cultured cells were treated with different concentrations of CXB(0,2.5?10~(-6),5?10~(-6),1?10~(-5),2?10~(-5),3?10~(-5),4?10~(-5),5?10~(-5)mol/L) and the proliferative status was evaluated by BrdU assay.The levels of vascular endothelial growth factor(VEGF) were measured by enzyme-linked immunosorbent assay(ELISA);the production of proliferating cell nuclear antigen(PCNA) and phosphoMAPK were measured by real-time reverse transcription-PCR assay;and the expression of PCNA,MAPK and phospho-MAPK protein was detected by Western blotting.Results: BrdU assay revealed that CXB inhibited cell growth in a concentration-dependent manner;the maximum inhibition rate([63.9?1.2]%) was found when cells were treated with 2?10~(-5) mol/L CXB for 24 h.VEGF secretion by cyst lining epithelial cell was reduced by CXB in a concentration and time-dependent manner.The mRNA and protein levels of PCNA,phospho-MAPK in CXBtreated group were lower than those in control group(with no CXB treatment).Conclusion: CXB can obviously inhibit the proliferation of cyst lining epithelial cell and the secretion of VEGF,which might be through interfering with the phosphorylation of MAPK and partly blocking MAPK signal transduction pathway.

Objective To improve the quality and santdard of medical French bilingual teaching and promote the learning ability of the students in medical universities. Medthods Analogy between English and French was applied to the intervention of bilingual teaching in the clinical students who were learning medical French. The advantages and disadvantages were analyzed.Results The students could apply their prior English to medical French learning and make progress in both languages. A series problems were found during the practice:arrangement of teaching contents, lack of teaching materials and shortage of teaching staff. Conclusion Analogy between English and French is an effective teaching method in both foreign language teaching and bilingual teaching. But the expected teaching goals can be achieved only by formulating reasonable teaching contents and strategies and mproving teachers’ quality.

This paper probes into the scientific research management at a teaching hospital,where three responsibilities are required in clinical service,medical education and research.The staffs＇ need for scientific research is discussed base on Maslow＇s need-hierarchy theory.An incentive system is also proposed for research and professional development of the hospital.

Object To study the technological conditions of refining ?-linolenic acid by short-path distillation (SPD). Methods According to the characteristic of SPD, the effect of the operating parameters, such as the distillation temperature, the system pressure, the feeding rate, and the agitating speed, were studied on the purity of ?-linolenic acid. Results The appropriate process conditions for the purification of ?-linolenic acid by SPD are: distillation temperature 90 ℃—120 ℃, pressure 0.3 Pa, feeding temperature 60 ℃, feeding rate 90—100 mL/h, and rotating speed 150 r/min. Conclusion Refining ?-linolenic acid by SPD is a simple and convenient method. It is easy to achieve industrialization.

Objective To investigate the Lemple-Zie complexity (LZC) characteristics in patients with Alzheimer' s disease(AD),mild cognitive impairment(MCI) and normal elderly,and the possibility of differentiating AD,MCI and normal elderly by LZC.Methods Electroencephalogram (EEG) of 30 AD patients,30 MCI patients and 20 normal elderly with eyes closed in rest state were recorded.In acquired EEG data,2048 points(10.14 s)of each channel were selected for LZC calculation by Matlab 7.0 software.Results (1) The average LZC values in AD,MCI and control groups were 0.396 ± 0.036,0.470 ±0.051,0.523 ±0.055 respectively.As compared with control group,the values in AD and MCI groups were decreased (F =43.092,P =0.000).(2) LZC values of AD in all channels (from 0.373 ± 0.042 to 0.430 ±0.083),whole brain,bilateral hemispheres,frontal and temporal areas were significantly lower than those in the normal group (from 0.498 ± 0.067 to 0.566 ± 0.059 ; t =3.602-8.747,P =0.000-0.010),and showed the significant decline(＞ 23％)in bilateral anterior middle temporal,frontal areas,the left parietal area.(3) Except the T6 channel,LZC values of AD groups in the remaining channels(from 0.373 ±0.042 to 0.418±0.063),the whole brain,bilateral hemispheres,frontal and temporal areas were significantly lower than those in the MCI group(from 0.455 ± 0.072 to 0.489 ± 0.063 ; in T5 channel,t =2.038,P =0.041,the others t=4.178-7.424,all P=0.000).(4) LZC values of MCI groups in the whole brain,bilateral hemispheres,temporal areas,parietal areas,the left frontal area were notably lower than those in the control group.Conclusions With the decline of the cognitive function,the EEG complexity value shows the parallel change.It suggests that the EEG complexity value can reflect the change of brain function in the duration from normal age to dementia in some degree; The MCI patients with abnormal LZC values in temporal and frontal lobes have a certain degree relation with the occurrence of AD.

Based on the research project of the French language teaching reform in Kunming Medical College——the Application and Analogy of Modern Foreign Languages Pedagogy,the successes and disadvantages of the visual-audio-oral course were studied.The teaching reform's purpose and focal points were primarily introduced.Then,the successful experiences of academic period arrangement,teaching materials selection,in-class instruction and students' academic achievements were elaborated.Finally,by making a comparison of students' French language proficiency between the present experimental classes and the former ordinary classes,a few disadvantages of the reform were interpreted.

The studies on the preparation technology,quality control and pharmacodyrnamics of Xi- aoyaosan Oral Liquid have been carried out. The experiment results suggested that,using the color reaction, TLC Identification and TLC-scanner could effectively control the intrinsic quality of oral liquid. Both possessed practical availability and their operations were simple Meanwhile,pharmacodynamic study also indicated that oral liquid could increase the peristalsis of stomach and bowel,the amount of consumed food and the body weight of mice,which was in conformity with the indication and efficacy of the original prescription.

98%. Conclusion The developed method is simple, reproducible and easy to operate. The solvent is cheap, with low boiling point and easy to recovery property.

Objective: To investigate the effect of celecoxib (CXB), a specific COX-2 inhibitor, on the proliferation of Han: SPRD-cy rats' renal cyst, and to probe into the related mechanism. Methods: Totally 57 3-week-old male Han: SPRD heterozygous(cy/+) rats were randomly divided into 3 groups (n=19): control group (fed with normal forage), low dosage CXB group (3 mg·kg -1·d-1) and high dosage CXB (10 mg·kg -1·d-1) treatment groups. The animals and their bilateral kidneys were weighed; the 2-kidney weight/total body weight (2K/TBW) ratio was calculated. The renal cystic index (CI), fibrosis index, and inflammatory cell infiltration in interstitium were observed by pathologic renal tissue slices. The co-expression of PCNA and COX-2 was analyzed by double immunofluorescence labeling technique and laser scanning confocal microscopy. The expression of PCNA and COX-2 protein was examined by Western blotting analysis. Results: The 2K/TBW ratio of the low dosage group ([1.10±0.009] %) was significantly lower than that of the control group ([1.33±0.02]%) at the 16-week old, (P<0.05). Compared with the control group, the inflammatory cell infiltration in other two groups was significantly decreased ([2.6±0.26], [2.8±0.31] vs [3.7±0.33]), P<0.05). The fluorescence intensities of COX-2, PCNA in the low dosage CXB group was significantly lower than those of the control group (P<0.05). Compared with the control group, the other two groups had significantly decreased expression of COX-2 ([0.326±0.011], [0.409±0.008] vs [0.814±0.012], P<0.05) and PCNA ([0.763±0.051], [0.925±0.042] vs [0.988±0.031], P<0.05). Conclusion: CXB at 3, 10 mg·kg -1·d-1 can reduce inflammatory cell infiltration and inhibit the proliferation of Han: SPRD rat's renal cyst, probably through inhibition of COX-2.

Objective To evaluate the localization and the correlation with cardiovascular risk factors of cerebral arteries damage in patients with transient ischemic attack (TIA). Methods Digital subtraction angiography (DSA) was performed in the 97 patients with TIA. The localization, the degree of stenosis, the numbers of the arteries involved and their correlation with the cardiovascular risk factors were analyzed. Results DSA showed occlusive findings of cerebral arteries in 91.8% of the patients, and 68 cases (76.4%) among them were involved more than one artery. The proximal internal carotid arteries (35.5%) and the proximal vertebral arteries (28.2%) were the most frequently involved in this series. And the number of the arteries involved and the degree of stenosis were positively correlated with the level of risk factors. Conclusion There is a high incidence of cerebrovascular stenosis in patients with TIA. Most of them are involved more than one artery. The degree of the stenosis is positively related with the quantity of the risk factors.