OBJECTIVE:To establish a simple and feasible method for rapid analysis of drug consumption patterns,and to im-prove the level of hospital pharmacy management. METHODS:Taking Motilium tablet as an example,the actual order amount da-ta of 24 months were analyzed by time series seasonal decomposition method;several key components of change trend were extract-ed by SPSS software,i.g. smoothed trend-cycle(STC),seasonal factors(SAF)and seasonally adjusted series(SAS). The varia-tion of components data series was observed to obtain the conclusion,and then compared with direct-vision method. RESULTS:The seasonal decomposition method had high prediction accuracy,the mean absolute percent error (MARE)(0.17) was less than that of error value by direct-vision method (0.24) significantly. CONCLUSIONS:This method can found drug consumption pat-terns quickly and accurately with the help of software. Based on this aspect,stock adjustment strategy can combine daily manage-ment and long-term dynamic management conveniently so as to improve the level of drug management.

Objective: To explore the role of v-akt murine thymoma viral oncogene homolog 2 (AKT2) and p27 in the chemoresistance of multicellular spheroids (MCS) of ovarian cancer to cisplatin. Methods: MCS of A2780 were formed by three-dimensional cell culture. Western blotting was applied to detect the expressions of AKT2 and p27. Then the interfering plasmid of AKT2 was constructed and transfected into the MCS of A2780 cells. The cell apoptosis after treatment with different concentrations of cisplatin was detected by flow cytometry (FCM) and the sensitivity of cells to cisplatin was evaluated by MTT assay. Results: Western blotting indicated that the expressions of AKT2 and p27 were higher in MCS than that in monolayer cells. The expressions of AKT2 and p27 in AKT2 shRNA-transfected MCS were significantly lower than that in MCS without transfection or transfected with empty vector. FACS analysis indicated that after treatment with various concentrations of cisplatin the apoptotic ratio of MCS was significantly lower than that in monolayer cells. AK72 shRNA transfection significantly increased the apoptotic ratio of MCS compared with those without transfection or transfected with empty vector. MTT assay showed that the sensitivity of MCS to cisplatin was increased after transfection with AK'12 shRNA. Conclusion: Interference with AKT2 shRNA down-regulated p27 and consequently reversed the drug resistance of ovarian cancer to cisplatin.

Objective: To investigate the expression of calcineurin (CaN) in breast carcinoma and to analyze the relationship between the expression of the member of CaN family and clinicopathological features. Methods: The expressions of CaN Aα, CaN Aβ and CaN B in 55 cases of breast carcinoma tissues and adjacent normal tissues were measured by Western blotting. The intracellular distribution of CaN proteins were detected by immunohistochemical method. Results: The expression levels of CaN Aα and CaN Aβ and CaN B proteins were markedly increased in breast carcinoma tissues compared with adjacent normal tissues. The relative intensities were 6.45-,4.67-, and 2.31-fold of the control group, respectively (P < 0.05). The expression levels of CaN Aα and CaN Aβ were related with TNM staging (III/IV) of breast carcinoma (P = 0.05). Conclusion: The continuous activation of CaN signaling pathway maybe play an important role in tumorigenesis of breast carcinoma.

As a major ingredient of high medical college education,experiment practice plays an important role for medical students in integrating the medical theory with practice,training their capability of analyzing and thinking to definitively solute the problems in their study.Microbiology experiment is characterized with strong comprehensiveness and manipulation,which requests the students both aseptic operation and consciousness of bio-safety.To promote the student's comprehensive diathesis and to improve the experiment teaching quality,several common problems of students in medical microbiology experiment practice were analyzed and discussed in this article.

Objective To explore the therapeutic effect of recombinant human growth hormone (rhGH) on the stature of children with Turner syndrome(TS) and analyze its influencing factors in treatment.Methods Twelve children with TS were selected in the outpatient department of West China second hospital of sichuan university between Jan.2004 to Jun.2007.They were diagnosed as TS with karyotypically.The average age was 4.9-16.9(11.28?3.64)years old.Every patient must be made sure to have normal liver function,kidney funtion and MRI for hypothalamus and pituitary before enrollment.All cases were never treated with growth hormone or estrin.Not all the patients had the same manifestations:12 cases with short stature,9 cases with cubitus valgue,8 cases with broad chest,4 cases with low posterior hairline,2 cases with webbed neck,11 cases without pubes and armpit hair,none with menarche.Every patient must be made sure to have normal liver function,kidney funtion and MRI for hypothalamus and pituitary before enrollment.rhGH was used subcutaneously 3-5 cm around navel eve-ry night before sleep.Average rhGH dose was 0.16 IU/(kg?d)[0.15-0.18 IU/(kg?d)],and height standard deviation score (HtSDS) was calculated and compared before and after rhGH treatment,and adverse reactions were observed.Results The duration of treatment was different:9 cases for 12 cases months,2 cases for 15 cases months and 1 case for 18 cases months.The average height increase rat was (0.55?0.15) cm / month.Difference of HtSDS existed before and after treatment by t test(t=2.87 P

Humans ; Male ; Mesylates ; poisoning ; Occupational Diseases ; Occupational Exposure ; adverse effects ; Young Adult

Objective To investigate the effects of cardiopulmonary bypass (CPB) with mild hypothermia on the pharmacodynamics of rocuronium in infants undergoing cardiac surgery.Methods Fifty ASA Ⅰ or Ⅱ and NYHA class Ⅰ or Ⅱ infants,aged 6 months-3 yr,weighing6-15 kg,undergoing repair ofventricular septal defect or atrial septal defect under CPB,were randomly divided into 2 groups ( n =25 each):profound hypothermia group and mild hypothermia group.The target temperature was controlled at 28-30 ℃ during CPB in profound hypothermia group,and at 32-34 ℃ during CPB in mild hypothermia group.Neuromuscular block was assessed by TOF-stimulation of ulnar nerve using with TOF-Watch accelerometer.Anesthesia was induced with midazolam O.1 mg/kg,propofol 2 mg/kg,fentanyl 6-8 μg/kg and rocuronium 600 μg/kg,and maintained with intermittent iv boluses of fentanyl and midazolam.The patients was tracheal intubated and mechanically venti1ated when the maximal depression of T1 was achieved.PETCO2 was maintained at 30-40 mm Hg.When T1 returned to 75％ of the control twitch,rocuronium 200 μg/kg was injected intravenously.The onset time,maximal N-M block time,clinical muscle relaxation time,and recovery index were recorded before CPB,during CPB and after CPB.Results Compared with profound hypothermia group,the onset time,maximal N-M block time and clinical muscle relaxation time were significantly shortened during CBP in mild hypothermia group ( P ＜ 0.05).Conclusion Compared with profound hypothermic CBP,mild hypothermic CBP can shorten the onset time and muscle relaxation time of rocuronium in infants undergoing cardiac surgery.

With the extensive use of information technology and the development of big data technology, the traditional treatment methods for cancer are not meeting our needs.The application of medical large data has now changed our tumor treatment model profoundly, but also brings a deeply cognition to the nature of malignant tumor.Development of medical big data analysis and management technologies are driving malignancy treatment model fromindividual treatment era into the precision medicine era, which allows us to change the prediction, diagnosis, treatment and monitor of malignancy.There are also a variety of challenges to resolve.

Objective To investigate the effect of L-carnitine (LCN) on heart function following myocardial contusion in mongrel dogs.Methods Twenty-four male mongrel dogs were randomly divided into myocardial contusion group,low dose LCN group (LCN1 group),and high dose LCN group (LCN2 group) with 8 dogs per group,according to the random number table.The model of myocardial contusion was established in all groups.LCN1 and LCN2 groups were respectively treated with 100 mg/kg and 200 mg/kg LCN for three consecutive days before the injury and afterward.Left ventricular systolic pressure (LVSP),left ventricular end diastolic pressure (LVEDP),left ventricular ± dp/dt were recorded 30 min before injury,and 30 min,1 h,2 h,4 h as well as 6 h after injury.Blood samples were withdrawn from femoral vein to detect the levels of creatine kinase isozyme (CK-MB),cardiac troponin I (cTnI),and malondialdehyde (MDA).Wall motion score index (WMSI) was observed using the ultrasound examination 1 day before and 5 hours after injury.Myocardial tissue was available at 6 hours after injury for the examination of content of adenosine triphosphate (ATP).Results LVSP and ± dp/dtmax appeared a downward trend after injury,while a gradual increase in LVEDP.And the changes were relatively smaller with LCN intervention,especially in LCN2 group.Levels of CK-MB,cTnI and MDA increased after injury,but the same time point showed lower levels in LCN groups (especially in LCN2 group) as compared to myocardial contusion group (P ＜ 0.05).WMSI increased 5 hours after the injury,but the increase was higher in myocardial contusion group (2.46 ±0.31) than that in LCN1 group (1.91 ± 0.23) and LCN2 group (1.85 ± 0.25) (P ＜ 0.05).There was no significant difference in WMSI between the two LCN groups (P ＞ 0.05).Level of ATP in myocardial contusion group was (11.3 ± 1.6) μg/g,which was improved after LCN intervention (P ＜ 0.05),and the improvement was much higherin LCN2 group than in LCN1 group[(21.3±3.9) ug/g vs (15.7±2.8)ug/g] (P＜0.05).Conclusion LCN can alleviate the adverse effect of myocardial contusion on cardiac function in mongrel dogs,which may be associated with the reduced energy imbalance induced by LCN.

Nowadays more and more attention has been paid to the problems about housing and health in China. Some investigations showed that people spent almost fourteen hours in their homes every day so the houses become the major places of daily activities. It is very important to solve the problems of what is the relation between the housing and health and how the housing influences the health of the people living in it. In the present paper the author summarized the overseas and domestic researches on the housing and health and pointed out that the research on housing and health in China is just in the preliminary phase.