Objective To survey the treatment effect of Inhibace on heart failure combined with arrhythmia. Methods Clinical diagnosis of cardiomyopathy 13 cases,carditis 11 cases,hypertensive heart failure 9 cases,cardiac infarction 9 cases,and rheumatice heart failure 2 cases were divided in NHYA Ⅱ 7 cases,Ⅲ 29 cases,Ⅳ 8 cases. There are premature beat in 11 cases,atrial fibrillation in 11 cases, ventricular tachycardia or ventricular fibrillation in 4 cases,and heart conductive block in 13 cases. Orally Inhibace 5 mg qd for two weeks and monitored by clincal synptoms and 24 hours Holter determination. Results The 41 cases of total 44 patients had the obvious effect(75%) and effectiveness(16%) accoording the health ministry heart diseases therapy standard,otherwhile 3 cases had no effectiveness(3/44).Conclusion Inhibace is a kind of ACEI,it ameliorate the circulating volumes,the loading and electrophysiology of heart muscles,that made the good effect in failure heart and decreased the ocurrence of arrhythmia.

OBJECTIVE:To analyze the current status of counterfeit in China and indicate the difficultie of identification.METHODS:The main characteristics of counterfeit drugs were discussed by representative cases.RESULTS & CONCLUSION:It is suggested that the department of the drug administrave should establish a more scientific,reasonable standardization procedure of conterfeit drugs identification aiming directly at the characteristics of counterfeit drugs,which was more sufficient,intensive and practicable legal evidence for defining,fighting and preventing counterfeit drugs.

Objective To investigate the expression of Toll-like receptor 4 (TLR4)in peripheral blood monocytes and its correlation with TNF-α and IL-6 in type 2 diabetes (T2DM)patients with neuropathy. Methods Healthy volunteers (NC,n=44),T2DM without neuropathy (n=44)and T2DM with neuropathy (n=44)were recruited in the present study.The expression of TLR4 mRNA and protein in peripheral blood monocytes was detected by RT-PCR and flow cytometry. ELISA was undertaken to measure the plasma concentration of TNF-αand IL-6. Results The levels of HbA1 c[(9.09±1.62)%vs(8.36±1.10)% vs (5.30±0.89)%],the protein expression of TLR4[(42.02±9.69)% vs (31.27± 6.87)% vs (11.96±5.54)%],the mRNA expression of TLR4[(2.98±1.06)vs (1.74±0.47)vs (1.12 ±0.52)],TNF-α[(8.75±3.14 )vs (6.27±3.64)vs(3.19±1.17)pg/ml]and IL-6[(3.63±1.81)vs(2.60 ±1.14)vs(1.54±0.58)pg/ml]in patients with diabetic neuropathy significantly increased compared with T2DM and NC group;TLR4 showed a positive correlation with TNF-αand IL-6(r=0.631,P <0.0001;r=0.447,P =0.0023). Conclusion TLR4 of monocytes may take part in the occurrence of systemic inflammation and has a close relationship with the development of diabetic neuropathy.

Uterine natural killer cells (uNK) are distinct from peripheral blood NK (pbNK) cells,which constitute up to 70％ of the decidual leukocyte population in the first half of pregnancy,and are considered to have a cytokine-secreting role rather than cytotoxic function.It plays an important role in trophoblast invasion and uterine spiral artery remodeling.As a result of imbalance of uNK cells,pathological pregnancy appeared.Herein,the origin of uNK cells and its function in pregnancy were veviewed.

There is no unity in judgment of obesity at home and abroad.WHO considered that there should have different evaluation criteria from childhood below and above 10 years and recommended "weight for height" be used for children under the age of 10 and body mass index(BMI)for children aged 10 to 24 years old.International Obesity Task Organization(IOTF) recommended BMI be suitable for young children for judgment overweight and obesity.Both of them had the same BMI standards for overweight and obesity for 18-year-old,25 and 30 kg/m2,respectively.But the Working Group on Obesity of China(WGOC)developed a criterion which cut-offs in 18 years were lower than that of WHO and IOTF,24 and 28 kg/m2,respectively.The treatment of obesity in childhood was different from that in adults.Many methods used for adults such as surgical liposuction,slimming drugs,hunger therapies and fasting could not be appropriate for childhood.A combined treatment in childhood including behavior modification,diet adjustment and exercise was recognized domestically and internationally.

Objective: To investigate the effect of insulin-like growth factor-1 (IGF-1) on expression of cystationine-β-synthase (CBS) and hydrogen sulfide(H2S) in rat adrenal pheochromocytoma cell line (PC12) and the possible signaling pathway. Methods: After PC12 cells were treated with 20,40, or 80 ng/ml IGF-1 for 24 h, fluorecence quantitative PCR was used to examined the expression of CBS gene, Western blotting analysis was used to detect the expression of CBS,ERK/MAPK protein expression, and sensitive sulphur electrode was used to analyze the levels of H2S gas. For inhibition studies, 25 or 50 mol/L PD98059, a specific ERK/MAPK inhibitor, was used 30 min prior to 80 ng/ml IGF-1 treatment: and the above parameters were examined again using the same method. Results: IGF-1 increased the expression of CBS, pERK1/2, and the levels of H2S; and PD98059 could inhibit the above effect of IGF-1. Conclusion: IGF-1 and its ERK/MAPK signaling pathway regulate the expression of CBS and levels of H2S.

Child ; Child, Preschool ; Humans ; Infant ; Smith-Lemli-Opitz Syndrome

Objective: To study the chemical constituents of the methylene chloride of Rubus chingii Hu.. Methods: Chromatography on silica gel column, Sephadex LH-20 column, and recrystallization technique were used to isolate and purify the compounds. Spectroscopy methods including EI-MS, ES-MS, 1H-NMR, 13CNMR, HMQC, and HMBC were used to elucidate the structures of compounds. Results: Ten compounds were obtained and 9 compounds were identified as: hexacosanol (I), β-sitosterol (II), 4-hydro-3-methlbenzal acid (III), 4-hydrobenzal dehyde (IV), oleanolic acid (V), stigmast-5-en-3-ol, oleate (VI), H-2-indenone, 2, 4, 5, 6, 7, 7a-hexahydro-3-(1-methylethyl)-7a-methyl (VIII), 4-hydroxy-3-methoxybenzoic acide (IX), and liballinol (X). Conclusion: Compounds I, III, IV, VI, VIII, and X have been obtained from Rubus chingii for the first time.

OBJECTIVE:To study the effects of arsenic trioxide(As 2 O 3 )on the encoding protein expressions of nm23and P53in gastric cancer cells and to study the anti-canecer mechanism of which in the tumor.METHODS:A control group and a test group were set up.The expressions of gene coding proteinum nm23and P53of the gastric cancer cells cultured in vitro by the action of As 2 O 3 were determined by immunohistochemistry method.RESULTS:The expression levels of nm23and P53in gastric cancer cells have been lowered compared with the control group.CONCLUSION:As 2 O 3 can exert its anti-tumor effect by decreasing the expression of gene coding proteinum of nm23and P53.

Objective　To investigate the relationship between nitric oxide (NO) secreted by ovarian granular cell and interleukin-1β (IL-1β), and their functions in estrogen synthesis. Methods　The human ovarian granular cells recovered from follicular fluid were cultured with IL-1β or NO donor. Results　The quantity of NO cultured with or without IL-1β addition were (537±23) μmol/L、(411±15) μmol/L respectively. The former was significantly higher than the latter (P<0.01). The estradiol, progesterone synthesis and aromatizing enzyme activity in the media with the addition of NO donor were (1 234±136) μg/L、(988±103) μg/L、(0.422±0.052) Ci/ml, while as they were (1 792±103) μg/L、(2 486±123) μg/L 、(0.833±0.012) Ci/ml, in the media without the addition of NO donor. The estradiol progesterone synthesis and aromatizing enzyme activity in the media with NO donor decreased significantly as compared with the group without NO donor (P<0.01, P<0.001, P<0.01). Conclusions　Ovarian granulosa cells may secrete NO, and the secretion may be regulated by IL-1β. They may be involved in the ovulation at the preovulatory stage and regulation of ovary steroids synthesis.