Objective To establish a method for determination of trace lead in drinking water by intermittent flow HG-AFS was developed. Methods The conditions for K3Fe(CN)6-KBH4-HCl system of generation of PbH4 were optimized and interference of coexisting ions were tested. Results The fluorescence signal of blank solution was decreased evidently by treating K3Fe(CN)6 with activated carbon and the detection limit was effectively improved. The interference of coexisting ions could be suppressed in some degrees in the presence of K3[Fe(CN)6]. Under the optimized conditions, the detection limit of this method was 0.10 ?g/L and RSD(n=6) was in the rang 0.4%-3.8%. The recovery rate was 95.0%-112.0%. Conclusion This method was accurate, simple, sensitive and suitable for determination of trace lead in drinking water.

Abscess ; pathology ; Adenoma ; metabolism ; pathology ; Adenoma, Sweat Gland ; metabolism ; pathology ; Biomarkers ; metabolism ; Breast Diseases ; pathology ; Breast Neoplasms ; metabolism ; pathology ; Female ; Fistula ; pathology ; Humans ; Keratin-7 ; metabolism ; Keratins ; metabolism ; Mucin-1 ; metabolism ; Nipples ; pathology ; Paget's Disease, Mammary ; metabolism ; pathology ; Receptor, ErbB-2 ; metabolism ; Sweat Gland Neoplasms ; metabolism ; pathology

Female ; Fertilization in Vitro ; Humans ; Integrative Medicine ; Medicine, Chinese Traditional ; Ovary ; drug effects ; Ovulation Induction ; methods ; Pregnancy ; Pregnancy Rate

Objective To evaluate the changes of left atrial(LA)function in peripartum cardiomyopathy(PPCM)patients using two?dimensional speckle tracking echocardiography(2DSTE). Methods Totally 35 PPCM patients and 35 healthy postpartum women(control group)were en?rolled in this study. Left ventricular end?diastolic diameter(LVEDd)and LA anteroposterior dimension(LAAD)were measured. The end?diastol?ic volume(EDV)and end?systolic volume(ESV)were obtained using biplane modified Simpson′s method. Cardiac output(CO)and left ventricu?lar ejection fraction(LVEF)were also calculated. E wave and A wave of mitral valve were measured,and correspondingly E/A ratio were obtained. LA longitudinal systolic strain(SS),systolic strain rate(s?SR),early diastolic strain rate(e?SR),and late diastolic strain rate(a?SR)were ob?tained by 2DSTE. Results There was no statistical difference of E wave between the two groups(P>0.05). Compared to the normal control group, LVEDd,EDV,ESV,LAAD,E/A were increased,while CO,LVEF,A,SS,s?SR,e?SR,a?SR were decreased in the PPCM group(P<0.05). a?SR was positively correlated with A wave in patients with PPCM(r=0.775,P=0.001). Conclusion LA reservoir,conduit and booster pump func?tion were decreased during PPCM. 2DSTE can easily and accurately assess these changes of LA function.

BACKGROUND:At the beginning of the 20th century a strength training method in the backward direction appeared:hip stress strength training method. There are many articles focusing on the concept, function, effect and mechanism of this training method. So far, relevant research has formed a unique branch of special strength training research directions.
OBJECTIVE:To conclude the concept, function, effect, features and mechanisms of hip stress technical strength training through the systematic analysis of literature concerning hip stress strength training. METHODS:CNKI (1998/2010) and Wanfang databases were searched by the first author for relevant articles. The keywords were“hip stress, strength training, trajectory control, exercise training, muscle strength, knee, physical fitness, bone”in Chinese and English. The obtained data were summarized using contrast method and logic analysis method.
RESULTS AND CONCLUSION:Total y 90 papers were retrieved, and final y 34 papers were included. The results showed that the hip technical strength training method is the most effective training method currently to cultivate the running, jumping and throwing back pedaling force after hip extension forces. It has a new training idea and special training equipments, which can effectively solve many sports difficulties in strength training and technical training and has the vital significance for the high-level sports training, skil s assessment and sports teaching. Hip stress special technical strength training research has formed a special research branch of special strength trainings, which wil play a more significant research role.

[Objective]To investigate the effect of using Minimully invasive percutuneous plate osteosunthosis to insert DHS for fixation of intertrochanteric hip fractures in elderly patients,in order to reduce operative trauma.[Method]To study retrospectivly 75 cases of the intertrochanteric fracture of elderly patients treated with DHS,these patients were treated from july 2004.7 to october 2005.10.MIPPO was used in 29 out of 75 cases,classic technique for fixation was used in other ones.[Result]All patients successfully discharged except two died because of fungal septicemia and lung embolism.Totally 73 cases were followed up from 7 to 21 months(averagd 12.3 months),one died of morbus internus in follow-up period.All the fractures had successful union,union time in average was 3.5 months,the diference between MIPPO group and classic incision group was not distinguished.Blood loss and transfusion in MIPPO group was less than classic incision group.Recovery of ESR and detumescence of the femoral was quickly in minimal incision group,time in bed was short.[Conclusion]Technique of minimal invasive fixation using DHS has little trauma and quick functional recovery for patients,therefore,this technique should be better for treatment of intertrochanteric fracture in elderly patients.

Abstract Objective:To explore antitumor effect of NDV HN. Methods: pIRHN nucleic acid vaccin was constructed and was transfectedto Hela cells. Westem blot was used to analyssize the expression of pIRHN nucleic acid vaccine in eukarytic cell. The mode of cell death was de-tected by fluorescence microscope, gel electrophoresis and TUNEL assay. The effect of pIRHN nucleic acid vaccine on the contents of siaha acidin the Hela cell was examined. Results: pIRHN nucleic acid vaccine could be expressed in the eukarytic cell. pIRHN could induced apoptosisafter HeLa cells were transfected. The effect of antitumor responses of pIRHN was correlated with the contents of sialic acid in tumor cells. Con-clusion: The reults of our experiment will provide the theory evidence for the anti tumor mechamism of NDV.

The Kato-Katz (KK) method is a well-known method of fecal examination for helminthiases. Its diagnostic sensitivity was found very high for clonorchiasis. The present study evaluated the correlation of Clonorchis sinensis egg counts by the KK method with those by direct smear and formalin-ether (FE) technique. The egg counts obtained by the KK method (Y) were correlated with the counts by direct smear (X) with the equation of Y = 659.4 + 0.266X (r2= 0.738), but not with those by the FE method. The present study demonstrated that the KK method and direct smear were useful for both qualitative and quantitative diagnosis of clonorchiasis, especially in the field.
Animals ; Cellophane ; Clonorchiasis/*diagnosis/parasitology ; Clonorchis sinensis/*isolation & purification ; Comparative Study ; Ether, Ethyl ; Feces/parasitology ; Formaldehyde ; Humans ; Parasite Egg Count/*methods ; Sensitivity and Specificity

Clinical data of 14 patients with iatrogenic duodenal injuries treated in hospital from January 2000 and January 2010 were retrospectively reviewed.Iatrogenic duodenal injuries were found intraoperatively in 9 cases,in whom repair or additional jejunostomy was performed and all were cured and discharged.In 2 patients the duodenal injuries were found within 24 hours postoperatively,1 was cured,another had low flow duodenal fistula and cured with conservative treatment.Duodenal bypass and extraoral drainage were performed in 2 patients whose duodenal injuries was found 72 hours after surgery and died from severe infection of retroperitoneal space and multiple organ failure respectively.One patient whose duodenal injury was found 7 d postoperatively suffered from septic shock and died in 4 h after admission.The results suggest that early detection and early management would result in satisfied outcome for patients with iatrogenic duodenal injuries,the first 24 hours are crucial.

BACKGROUND: Tooth intrusion easily leads to root resorption. Previous studies regarding orthodontic treatment-caused tooth root resorption or retrospective clinical studies based on X-ray films have great errors in outcome evaluation because of intrusion force which cannot be precisely controlled. OBJECTIVE: This study established dog models of mini-implant anchorage for incisor intrusion to observe the histological changes of tooth root and periodontal tissue and to evaluate the feasibility and safety of mini-implant anchorage for incisor intrusion. METHODS: Nine dogs were assigned to one control group (n = 1) and four experimental groups per time to sacrifice (1, 2, 4 and 12 weeks; n = 2 dogs for each experimental group). No force was added to the control group. In the experimental groups, mini-implant as an anchorage was placed in the buccal alveoli between maxillary second and third incisors on each side. A traction force of 100 g was imposed to each side to intrude the maxillary first and second incisors on each side. At 1, 2, 3, and 4 weeks (traction force was imposed for 4 weeks and after withdrawal of extraction force, mini-implant was retained in place for 8 weeks), dogs were sacrificed. The first and second incisors together with gingival and alveolar bone were completely resected to prepare histological specimens. Following hematoxylin-eosin staining, histological changes of tooth root and periodontal tissue were observed. RESULTS AND CONCLUSION: Compared with the control group, in the 1-week group, histological changes were primarily at the root tip and alveolar ridge crest, alveolar bone and cementum were absorbed and peridental membrane presented glassy degeneration in local region; in the 2-week group, bone resorption degree and range were obviously enlarged, and bone resorption developed from root tip, root middle part to cervical part; in the 4-week group, bone resorption was still active and the glassy degeneration of peridental membrane disappeared; in the 12-week group, significant improvement in alveolar bone and cemental surface was observed, bone lacuna had deposition of newly formed bone, and peridental membrane was orderly arranged. These findings reveal that in the mini-implant anchorage for dog incisor intrusion, early histological changes primarily appear in the root tip and alveolar ridge crest, presenting as alveolar bone and cemental resorption and the glassy degeneration of the peridental membrane. Bone resorption extent and range expand with the persistence of traction force. After withdrawal of traction force, tooth root and periodontal tissue were gradually repaired