Objective To observe the effect of lactoferrin supplementation combined with standard triple therapy on eradication rate of Helicobacter pylori (H.pylori) and its adverse reaction in children. Methods A total of 90 children who were diagnosed with H.pylori infection from January 2012 to January 2013 and had never received eradicative treatment were ran-domly divided into two groups. Forty-five children in control group received the standard triple therapy and another 45 children in observation group received lactoferrin supplementation combined with standard triple therapy. Adverse reaction during the treatment was recorded and eradication rate of H.pylori at 4 weeks after treatment was observed. Results The eradication rate of H.pylori in observation group (91.11%, 41/45) was higher than that in control group (73.33%, 33/45) and the adverse reaction rate in observation group (4.44%, 2/45) was lower than that in control group (20.00%, 9/45).The differences were significant (P<0.05). Conclusions Lactoferrin supplementation combined with standard triple therapy can increase H.pylori eradication rate and reduce the occurrence of adverse reaction.