In order to observe the effects of pentoxifylline and povine iodine on preventing postoperative intestinal adhesions (POIA),the authors used eighty SD rats as the models of intestinal adhesions and divided them into four groups:control group,pentoxifylline group,povine iodine group,and combining drugs group Each group contained twenty rats The results showed that the adhesions of each group were different and the consequence of adhesive degree (from serious to slight) was control group,povine iodine group,pentoxifyllin group,and combining drugs group It is demonstrated that pentoxyfilline and povine iodine have significant effects on preventing POIA; and the combination of the two drugs can have a synergetic effect on POIA

Objective To investigate the application of the double loop double button plate fixation on treatment of patients with complete acromioclavicular joint dislocation.Methods Twenty-six cases with Rockwood type Ⅲ complete acromioclavicular joint dislocation were selected as our subjects who hospitalized from Jan.2011 to Jul.2013 and underwent using dual loop button plate technology of double fixation and reconstruction of coracoclavicular ligament fixation.There were 19 males and 7 females.Their age ranged from 18 to 62 years old and average was 30.2 years old.Adopt Lazzcano standard score was used to evaluate the shoulder joint function.Results X-ray examination showed the acromioclavicular joint of all patients were fixed in right place.Shoulder function was back to normal after 1 to 2 months and no pain or mild pain were showed in some patients.Twenty-four cases were followed up for 6-24 months,and the average followed up periods was 18.2 months.According to Lazzcano standard score to assess the function of shoulder join.Twenty cases were excellent and 4 cases were in good.The excellent and good rate was 100％.Conclusion Dual loop button plate technique is proved to be a good approach in the treatment of complete acromioclavicular joint dislocation with small trauma,stable fixation.Meanwhile patients can perform early functional exercise and no recurrent dislocation without the need of internal re-fixation.

Objective To investigate the method and optimum conditions of electric transfection,and the major influential factors of electransfection efficiency and the survival rate of dendritic cells. Methods RNA was extracted from human hepatocarcinoma cell line(Bel 7402).Purified monocytes as precursor DC-s(pDC-s) were separated from human peripheral blood cells(PBMCs) by density gradient centrifugalization with lymphocyte gradation fluid and adherence method,pDCs were incubated in RPMI-1640 medium containing rhGM-CSF(8?10~5IU/L) and rIL-4(5?10~5IU/L) for 7 days and made them fully differentiate into immature DCs(imDCs).The total RNA human hepatocarcinoma cell and green fluorescent protein(GFP) were electransfected respectively into imDCs by electroporation apparatus with different electric voltages,times of impulse,cell concentrations,temperatures and electroporation buffers.Numbers of green fluorescence positive cells and the total cell number were counted respectively under fluorescent microscope,and visible light microscope.One day after the electric transfection,the cells were stained with 0.4% trypan blue,and electransfection efficiency and the cell survival rate were counted. Results Electransfection efficiency was increased to the highest value,up to about 49.7% when imDCs with the concentration of 5?10~6 cells/ml were mixed with 40?g-total RNA of human hepatocarcinoma cell,the electric voltage of electroporation apparatus was set at 300V,and the time of impulse was 500Us.Conclusion Electric transfection provides a technical possibility to make human hepatocarcinoma RNA into imDCs.The major influential factors of the electransfection efficiency were electric voltage and impulsing time.As receptor cells,the imDCs growing condition was also an important influential factor.

Vascular endothelial growth factor (VEGF) have multiple protective effect on the nervous system which related to angiogenesis,neurogenesis,directly neurotrophic protection and antiapoptotic mechanisms.The study of the nervous system of VEGF focus on tumors,trauma,stroke,autoimmune diseases and neurodegenerative diseases.The study of VEGF related to epileptic seizures is relatively rare.This paper is to make a review about the neuroprotective effect of VEGF on epileptic seizure.

Objective To evaluate the administration of dinoprostone suppositories in induced labor for full-term pregnancy women with hypertensive disorders.Methods One hundred and nine full-term pregnant women admitted in Jiaxing Maternal and Child Health Care Hospital from February 2010 to December 2011 were included in the study.The subjects included 43 cases with pregnant hypertension (group A),36 cases with mild preeclampsia (group B),30 cases with severe preeclampsia (group C),and 30 full-term normal puerpera were also included (group D).Dinoprostone suppositories were placed in the vagina for induction.The cesarean delivery rate and perinatal prognosis were compared among the four groups.Results The cesarean delivery rate in group C was higher than other 3 groups; there was no significant difference among groups A,B and D.The rates of fetal distress and postpartum hemorrhage in group C were higher than those in other 3 groups; there were no significant differences in rates of fetal distress and postpartum hemorrhage among group A,B and D,while there was no difference in rate of neonatal asphyxia among all groups.Conclusion Dinoprostone is effective and safe in inducing labor for full-term pregnancy women with hypertensive disorders.

Objective:To evaluate the clinical outcomes of TCM external treatment for atlantoaxial osteoarthritis.Methods:36 Atlantoaxial osteoarthritis patients were divided into a treatment group(18 cases) and a control group(18 cases).Patients in the treatment group were given oral western medicine and external treatment of TCM,including external medicine and osteopathtic manipulation;while those in the control group were given only oral western medicine treatment.All the patients were assessed with visual analogous scale(VAS) before and after treatment.Results:The pain of all patients was decreased after treatment.The VAS score in both groups were reduced after treatment and the difference was significant as compared with that before treatment(P

Postherpetic neuralgia (PHN) is a difficult medical issue and symptomatic treatment with medication is common. One case of PHN was cured by nerve avulsion and microtherm plasma nerve block. The male patient was 48-year-old with PHN on the right side of the nose, suffering recurrent pains within one year. The symptoms occurred irregularly and lasted for several minutes to hours every time. Electroacupuncture and Chinese medicine treatments in other hospitals made little efficacy. Physical examination showed skin of right side of the nose and nasal mucosa was normal and all laboratory reports confirmed negative. After microtherm plasma treatment in nasal cavity and corresponding area of nasal septum, the pain disappeared.
Humans ; Male ; Middle Aged ; Nerve Block ; Neuralgia, Postherpetic ; therapy ; Nose ; pathology

The effects of sepsis on the liver include hypoxic hepatitis caused by ischemia and shock, liver cell damage caused by excessive inflammation, and cholestasis caused by changes in bile metabolism. Among them, cholestasis is a common complication in patients with sepsis. Cholestasis and jaundice in patients with sepsis are associated with an increased risk of infection, leading to an increased mortality rate. So far, the exact mechanism of sepsis with cholestasis is still unclear, so this article briefly reviews the mechanism of cholestasis during the early stage of sepsis.

BACKGROUND: "Toxic impaired brain retinervus" is the important mechanism of acute cerebrovascular diseases, mainly presented by cerebral microvascular impairments.OBJECTIVE: To establish cell model of various degrees ischemia-reperfusion injury and expos to qingkailing medication, in order to observe the protective role of qingkailing injection on human umbilical venous endothelium ECV304 (huveECV304)DESIGN: Randomized controlled study based on cultured cells.SETTING: Department of Combination of Traditional Chinese Medicine and Western Medicine, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology.MATERIALS: This study was carried out at Center Laboratory of Dongzhimeu Traditional Chinese Medicine College, Beijing from February to April 2002. huveECV304 were randomly divided into 7 groups, namely normal group (NG), ischemic-ypoxia group (IR group), ischemia 6 hours reperfusion 12 hours control group (I-6-hour-R-12-hour group), ischemia 24 hours reperfusion 12 hours control group (I-24-hour-R-12-hour group),ishcmeia 24 hours reperfuiosn 12 hours low dosage qingkailing group (I24-hour-R-12-hour-LDQ group), ischemia 24 hours reperfusion 12 hours middle dosage qingkailing group (I-24-hour-R-12-hour-MDQ group), ischemia 24 hours reperfusion 12 hours high dosage qingkailing group (I-24hour-R-12-hour-HDQ group) and ischemia 6 hours reperfusion 12 hours middle dosage qingkailing group (I-6-hour-R-12-hour-MDQ group). The qingkailing injection mainly consists of cholaic acid, hyodeoxycholic acid,baikal skullcap root, cape jasmine fruit and so on.were collected, and primary culture medium were removed in I-6-hour-R-12hour group and I-24-hour-R-12-hour group and added with non-glucose Earle's solution, the cells in I-6-hour-R-12-hour group were cultured in ischemic incubator in 37 ℃ for 6 hours, for 24 hours in I-24-hour-R-12-hour group and then reperfusion-cultured with Earle's medium replaced by nonLDQ group, I-24-hour-R-12-hour-MDQ group and I-24-hour-R-12-hour-HDQ group, IR injured cells were exposed to qingkailing in dosage of 5, 10,by using MTT colorimetric assay, lactate dehydrogenase leaking rate was determined by using lactate dehydrogenase reagent kit, cell apoptosis rate was detected with FCM and statistically analyzed with Cellquest software.apoptosis rate.were found gathering closely, well adhering to the wall and completely stretching, cell surface was smooth and more cleavage cells were observed,cell grew over all the bottom displaying typical "slabstone signs"; but no obvious cell morphological changes were observed in control group and Ⅰ24-hour-R-12-hour LDQ group; while in I-24-hour-R-12-hour control group, cell were found scarcely distributed and mostly drop off, a great deal of tumid round injured cells and broken cell pieces were observed,adhering cells were shrunk obviously with pseudopod stretching out, less cleavage cells were observed; In I-24-hour-R-12-hour MDQ group, cells were found well adhering to the wall, the surface was smooth without cell: Comparing to I-24-hour-R-12-hour control group, the survival rate were markedly increased in I-24-hour-R-12-hour LDQ group, I-24-hour-R12-hour MDQ group [(42.5±2.8), (72.9±8.0), (80.3±12.7)%, P ＜ 0.01], but obviously descended to (10.1 ±0.4)% (P ＜ 0.01) in I-24-hour-R-12-hour ing rate of ECV304 cell: Comparing to I-24-hour-R-12-hour control group,it was found markedly increased in I-24-hour-R-12-hour LDQ group and Ⅰ24-hour-R-12-hour MDQ group [(6.6±1.4), (2.4±1.4), (2.4±0.5)%, P ＜ 0.01], but significantly ascended to (16.1±2.2)% in I-24-hour-R-12-hour rate of ECV304 cell: Comparing to I-6-hour-R-12-hour control group, it was obviously increased in comparing to I-6-hour-R-12-hour MDQ group [(2.2±0.5), (1.5±0.4)%, P ＜ 0.05]; comparing to normal group, it was found significantly increased in I-24-hour-R-12-hour control group and I-24-hourR-12-hour MDQ group [(0.9±0.2), (5.5±2.5), (9.9±4.3)%, P ＜ 0.01]; Moreover the increment in I-24-hour-R-12-hour control group was significantly higher than I-24-hour-R-12-hour MDQ group (P ＜ 0.01).ECV304 with various degree IR injury, but which displaying tow opposite cells due to I-6-hour-R-12-hour exposure was mainly presented by lowered cell apoptosis rate; But severer injury of ECV304 cells due to I-24-hour-R12-hour exposure was presented by increased cell apoptosis rate and lowon and cell membrane would not break in apoptostic cells and no internal content effused, therefore the surrounding cells would not be affected,which increasing the survival rate and displaying protective role for most cells.

BACKGROUND: It has been testified the interrelation between many microelements and mental diseases by determination of serum concentration or single determination of several microelements. But there are lack of definite data in comparison between big samples and various microelements.OBJECTIVE: To determine the levels of 6 microelements (Pb, Cd, Al, Zn, Cr and Ni) in hair of schizophrenic patients and compare with normal persons.DESIGN: Case-control, single center and big sample observation was designed.SETTING: Department of Psychiatry, Nanning Fifth People's Hospital.PARTICIPANTS: Totally 440 cases of latest inpatients of schizophrenia were selected from the ward of Department of Psychiatry, Nanning Fifth People's Hospital from April to December in 2000. They had not taken drugs for anti-mental disease yet, of which, 260 cases were male, 180 cases female; aged averagely (34±13) years, 122 cases ≤ 29 years old, 236 cases aged varied from 30 to 49 years and 82 cases ≥50 years old. In the control group, 218 persons were selected from the staffs of Nanning Fifth People's Hospital, of which, 128 cases were male and 90 cases female; aged averagely (37±11) years, 56 cases ≤ 29 years old, 121 cases aged varied from 30 to 49 years and 41 cases ≥50 years old. All of the participants were in the know and agreed with the experiment.METHODS: WFX-12 type atomic absorption spectrophotometer was used to determine in parallelism the levels of Pb, Cd, Al, Zn, Cr and Ni in hair of 440 cases of schizophrenic patients and 218 cases of normal persons.Multi-factor logistic regressive model analysis was carried out on microelement levels between patient group and normal group.ment levels in hair concerning to sex and age among schizophrenic papatients and normal persons.RESULTS: Totally 440 cases of schizophrenia and 218 normal persons enanalysis: The odds ratios (OR) were 1.07, 0.32, 0.99, 0.99, 1.74 and 0.63 successively between schizophrenia and the levels of Pb, Cd, Al, Zn, Cr and Ni;mong schizophrenic patients: The levels of Cd and Zn in hair of male patients were higher than those of females [(0.46±0.40) μg/g, (0.33±0.27) μg/g;(128.40±38.95) μg/g, (118.95±35.67) μg/g, F=14.84, 6.71, P ≤ 0.01].The levels of microelements of patients at 3 different age divisions were tween schizophrenic patients and normal persons: The levels of Pb and Cr in hair of schizophrenic patients were higher than those in the control group[(10.22±7.79) μg/g, (8.44±7.57) μg/g;(1.36±0.71 μg/g, (1.18±0.46) μg/g,F=6.21-8.52, P ≤ 0.01], but the levels of Cd, Zn and Ni were lower than those in the control group [(0.41±0.36) μg/g, (0.51±0.40) μg/g;(124.53±37.89) μg/g, (139.21±34.52) μg/g;(0.90±0.66) μg/g,(1.05±0.53) μg/g, F=6.21-8.52, P ≤ 0.01].CONCLUSION: A certain of correlation presents between the levels of Pb, Cd, Al, Zn, Cr and Ni in hair and schizophrenia. The levels of Cd and Zn are different concerning to sex, in which, those of male were higher than female. The difference was not significant in the levels of 6 microelements concerning to age in patient group. The levels of Pb and Cr in hair of schizophrenia were higher than the normal persons, but the levels of Cd, Zn and Ni were lower than the normal persons.