A total of 100 neurons were recorded from the nucleus raphe magnus (NRM), of which 59 were identified as noxious-exaitation neurons; 30 as noxioes-inhibition neurons; 5 as non-noxious neurons; and 6 as non-responsive neurons Seventy neurons were further tested with electro-acupuncture (EA). 69.77% of the noxious-excitation neurons were excited and 68.42% of the noxious -inhibition neurons inhibited by EA. Stimulation of the periaqueductal gray (PAG) could activate the majority of the EA-response neurons. Systemic morphine could also affect the EA-response neurons through the following three patterns: excitation, inhibition and no-effect. No signilicant correlation between the response patterns of EA and morphine was found. The results indicate that the effect of NRM in EA and morphine analgesia is only partially overlapped.

In the hospital administration,the establishment and the implementation of the strategic planning of the hospital occupies a very important position.This paper,combined with the actual situation of a children's hospital in Chongqing,describes the hospital rational use of three level management system of the hospital,office,department,in order to get the full investigation of the national macro policy,the development of the medical industry and the status of the hospital and other aspects,and then supports efficient workflow to complete the hospital strategic planning.This paper also focuses on the target implementation,program implementation and project implementation of three levels,in order to illustrate the process for the implementation of the strategy to provide a reference of.hospital strategic planning formulation and implementation.

Objective:To analyze Bufei Huo Xue Tang combined with Western medicine for chronic obstructive pulmonary disease( COPD) and its effect on airway inflammation reduction and lung function improvement. Methods: 100 patients with stable COPD in our department from February 2013 to March 2015 were randomly divided into combination group and control group. The control group received conventional oxygen,salmeterol and fluticasone propionate inhalation powder(1 absorption /times,2 times /day) and expectorant treatment. Combination group were based on the addition of lungs Bufei Huo Xue Tang with a course of 12 weeks. The clinical efficacy and α1-AT and IL-8 levels in two groups of patients were compared. Results:The cough,sputum,difficulty breathing, wheezing,wheezing symptom score in the combined group after treatment was significantly lower than the control group ( P<0. 05 ) . FVC,FEV1 and FEV1 /FVC in the combined group after treatment was significantly lower than those in the control group(P<0. 05).α1-AT and NO in the combination group was significantly higher after treatment. IL-8 and TNF-α was significantly lower than the control group(P<0. 05). BODE scores in the combined group after treatment were significantly lower than the control group(P<0. 05). Conclusion:Bufei Huoxue soup combined with Western medicine for COPD can inhibit inflammation,improve clinical symptoms,lung function and quality of life.

Delayed cerebral vasospasm is the leading cause of death in patients with subarachnoid hemorrhage. A good in vivo animal model of cerebral vasospasm after subarachnoid hemorrhage helps to understand the occurrence, development of this cerebral vasospasm, and the complicated and various pathophysiological mechanisms. It will provide important information for clinical interventions

Hepatocellular carcinoma(HCC)shows high malignancy and metastasis. Hepatitis B virus X protein (HBx), a virus protein coded by hepatitis B virus, plays an important role in HCC metastasis. Recently, it was reported that HBx activates metastatic signal-pathways through MMPs, VEGF, uPA etc.,and promotes metastasis of hepatocellular carcinoma.

Objective To discuss the procurement managerial technique standard for medical equipment importation.Methods The procurement managerial technique standard for medical equipment importation was studied from the aspects of flows for international bidding,declaration and operation as well as related specifications.Results The imported medical equipment according with the procurement managerial technique standard,gifted with a high cost performance,satisfied the requirements of the nation and the hospital.Conclusion The managerial technique standard can promote the procurement of imported medical equipment.

Objective To investigate the distribution and significance of CD20 positive cells in the early human embryo. Methods Distribution and morphology of CD20 positive cells in 10 specimens of human embryo aged from 6 to 7 weeks were brown,and studied with immunohistochemical method. Results 1.CD20 positive cells appeared in the liver of early human embryo.2.The immunohistochemical positive substances were brown,and found chiefly in the nuclei of B cells as unevenly-distributed granules,and were not detected in the cytoplasma and on the cell membrane.Conclusion The accuracy of location and distribution of CD20 positive cells in the nuclei of B cells of early human embryo liver may provide an important clue for further exploration of the functional mechanism of CD20 in the process of B cell proliferation and differentiation.

AIM: To develop a method for optimizing of supercritical fluid extraction (SFE) of paeoniflorin. METHODS: On the basis of one-factor test and the Box-Behnken central-composite design response surface with 3 factors and 3 levels was adopted. A second order quadratic equation for SFE of paeoniflorin was built. Response surface and contour were graphed with the extraction rate of paeoniflorin as the response value. Based on the analysis of the response surface plots and their corresponding contour plots,the effects of extraction pressure,extraction temperature,and methanol concentration were explored. RESULTS: By using the new method discussed above, the optimum extraction condition was obtained as follows: 18. 9 MPa extraction pressure,extraction cosolvent temperature 59. 9 ℃,68. 5% methanol was chosen as cosolvent with the flow rate of 0. 4 mL/min,and extraction time 60 min. CONCLUSION: Under the optimized condition,extraction rate of paeoniflorin on theoretical value is 7. 85 mg/g.

Objective Through the statistical analysis of the student assessment on curriculum quality,through the observation of the current curriculum teaching situation and its cheracteristics to promote the curriculum construction effectively and improve the medical teaching quality.Methods The current study is carried out among 2 841 undergraduates who study in varied majors and different grades in a medical university.A table of Student Evaluation of Course Teaching Quality developed by the researcher is applied to the questionnaire survey and statistical analysis including comparation means test and correlation tests are adopted in the present study to analyze the course teaching quality.Results Initially.the National Selected Model Courses show no significant difference from the common courses;secondly,three variables including teaching content adopting the course syllabus,good professional qualification and teaching capability of teachers and advanced teaching methods and techniques ale highly scored among ten variables;thirdly,the correlation coefficient of the variable of improving self-learning ability and total score is statistically significant and it can be observed higher than that of other variables and total score.Conclusion Establishing student-centered curriculum evaluation and construction of long-effect mechanism,and developing the students'ability of autonomous learning ale important to improve the quality ofthe course.

BACKGROUND:With elevated medical levels, limb salvage surgery for limb malignant tumor is valuable day by day. At present, the limb salvage surgery has substituted amputation and becomes mainstream and development direction of present surgical treatment of limb tumors. However, so far, there are no unified surgical indications for proximal femur tumor, which are controversial. OBJECTIVE:To investigate the efficacy of artificial total hip arthroplasty combined with extended resection of tumor sections for proximal femoral bone tumors. METHODS:Patients with proximal femoral bone tumors in the Second Xiangya Hospital of Central South University were selected and divided into control group and observation group, with 30 patients in each group. According to the disease, lesion curettage, aneurysm wal inactivation, autologous and (or) al ogeneic bone, mixed bone graft and bone graft fixation were selected to treat the control group. The observation group patients received extended resection of tumor sections and total hip arthroplasty. The operative time, intraoperative blood loss, hospital stay and joint function of two groups were compared. 2 years later, patients were revisited. Metastasis and recurrence rate, death rate and quality of life of two groups were compared. RESULTS AND CONCLUSION:There was no significant difference in intraoperative blood loss and surgery time between the two groups (P>0.05). However, hospital stay of patients in the observation group was shorter than the control group. The excellent and good rate of recovery of joint function (83%) was higher in the observation group than in the control group (53%). The metastasis and recurrence rate within 2 years after surgery (7%) and death rate (3%) were lower in the observation group than in the control group (30%, 23%). Various indicators of quality of life of patients in the observation group were significantly better than the control group (P<0.05). These results confirmed that artificial total hip arthroplasty in the treatment of proximal femur tumors is safe and effective.