To attach importance to practice teaching in clinical microbiological analysis,the overall planning,research-on-research and systematic training must be carried out.

Objective The study discussed the underlying mechanism of the impact of perceived social support on individual learning behavior from the perspective of the psychosocial impact theory .Methods 224 students majored in public management were involved ,and the dada was collected by questionnaires ,then the conceptual model was tested by moderate effect sequence of statistical analysis .Results The findings were as follows :Firstly ,perceived social support had a significant effect on individual learning behavior (β=0 .332 ,P<0 .01) .Secondly ,the relationship between perceived social support and individual learning behavior was moderated by self efficacy (β= -0 .216 ,P<0 .01) .Conclusion Perceived social support and self efficacy have moderate effect on individual learning behavior .

Objective To make an investigation for autoantibodies detection in China. Method Forty-eight hospitals were included by mail or Email. The items of QC include ANA, anti-ENA antibody, anti-dsDNA antibody, anti-mitochondria antibody and anti-smooth muscle antibody. The distribution of samples and analysis of the testing results was double-blinded. Result The correct rate of ANA, anti-ENA antibody, anti-dsDNA antibody, anti-mitochondria antibody and anti-smooth muscle antibody were 47%, 70%, 46%, 95%and 40% respectively. Conclusion The overall results are not optimistic. The quality of detecting kits is not all good, neither level of technicians. This condition should be changed by selecting better methods and improving understanding.

Objective To analyze the outcome of patients with acute obstruction of left colorectal cancer treated by modified antegrade colonic lavage with primary tumor resection and anastomosis.Methods From April 2002 to April 2007,112 patients with acute obstruction of left colorectal cancer underwent surgery.During the operation the left colon was exteriorized and placed into a sterilized plastic bag to protect the surgical field from contamination,then a catheter was inserted via the appendix,and after antegrade colonic lavage,primary resection and anastomosis was performed.Results Tumor resection and primary anastomosis was successfully done in the 112 cases.Postoperatively,1 case had anastomotic leak which healed after reoperation with proximal colostomy,and one patient died.Conclusions Modified antegrade colonic lavage is a simple procedure,the bowel can be rapidly decompressed with essentially no contamination,and has a high level of bowel cleansing.It is possible to safely perform primary resection and anastomosis for left colon cancer after the modified antegrade lavage.

Adult ; Aged ; Chronic Disease ; Female ; Gram-Positive Cocci ; isolation & purification ; Humans ; Male ; Middle Aged ; Nasopharyngeal Neoplasms ; microbiology ; radiotherapy ; Radiotherapy ; adverse effects ; Sinusitis ; etiology ; microbiology ; Young Adult

Objective To investigate clinical effects of transurethral resection of bladder tumor (TURBT) combined with postoperative perfusion of mitomycin C to urinary bladder on superficial bladder cancer. Methods 32 patients with superficial bladder cancer were treated by TURBT and discontinuous perfusion of mitomycin C (20mg/20ml) to urinary bladder. Results 32 cases of the superficial bladder cancers all were once ablated. 8 cases relapsed in 6 months to 2 years of postoperation, which were treated by TURBT and discontinuous perfusion of mitomycin C to urinary bladder again, and relapse was not found from postoperation to present. Conclusions Treatment of TURBT combined with perfusion of mitomycin C on superficial bladder cancer is repeatable, simple, safe and effective.

miR-320 is a newly discovered microRNA .In recent years, the abnormal expression of miR-320 has been found in various kinds of tumors .Therefore, its function and molecular mechanism in the tumorigenesis and tumor progression has getting more and more attention .

Objective To investigate non-psychiatric doctors' knowledge and attitude toward depression. Method Self-made Depressive Knowledge Questionaire (DKQ), which includes 24 items covering general knowledge, symptoms and other related questions of depression, were used in 346 non-psychiatric doctors who came from different grade general hospitals of Hunan. Results Average score of DKQ was 13.53±2.08,and corrective answering rates to different questions were 14.5％～95.4%.There were some associations between DKQ's score and grade of hospitals, subjects' education and attitude to depression. Conclusion It is necessary to re-educate non-psychiatric doctors with depressive knowledge.

Objective To discuss the method to increase the success rate of stomach tube insertion for patients with indwelling nasotracheal tube in order to shorten the time of insertion operation and reduce the discomfort of patients.Methods Seventy patients with nasotracheal tube were randomly divided into the observation group(38 cases)and the control group(32 cases).When the operator for the observation group inserted stomach tube to 18～22 centimeters deep,the nurse pumped the gas out from the tracheal catheter aerocyst.After the stomach tube reached stomach the tracheal catheter aerocyst was aerified again.The control group did not release the gas from tracheal catheter aerocyst when inserting the stomach tube.The first intubation success rate and time needed for intubation were compared between the two groups.Results The first intubation success rate of the observation group was higher than that of the control group(P＜0.05).While the time needed for intubation was shorter than that of the control group(P＜0.01).Conclusions Method used by the observation group not only increased the first intubation success rate,but also shortened time needed for intubation.This method can alleviated pain of patients and worth applying widely in clinic.

Objective: To investigate the relationship between academic achievements of high school students and the results of California Psychological Inventory (CPI) of them. Method: Seventy-one higher school students were tested by CPI-RC (revised Chinese version). Their academic achievements were recorded. Results: Nine of the 23 CPI traits, such as Re (responsibility), So (socialize), Wb (comfortable), Ac (achievement), Py (psychologized), Cm (communality), To (tolerance), Sc (self-control), V3 (self accomplishment) had significant correlation with academic achievements (r=0.24～0.33, p＜0.05). Students with different prefer (literal arts or science) showed different CPI traits. Students with better academic achievements had higher scores on So, Sc, Wb, To and V3. Conclusion: CPI traits have some relationship to academic achievements of high school students and to their academic preference.