Objective:To investigate the expression rule of Cyclin-E in endometrium during the early pregnancy and its role in blastocyst implantation in mice.Methods:RT-PCR and immunohistochemistry techniques were applied to detect Cyclin-E gene and protein expression in endometrium from pregnant mice on days 1～7.In addition Anti-cyclinE antibody was injected into horns of uterus of day 3 pregnant mice to observe the numbers of implanted blastocysts.Results:Expression of Cyclin-E gene and protein in pregnant mice were steady increasing since day 2 and reaching maximal level on day 5 of pregnancy.Injection of Anti-cyclinE antibody could decrease the numbers of implanted blastocyst compared with that of saline-injected group.Conclusion:Cyclin-E were persistently expressed in mouse endometrium during the early pregnancy and might be concerned with proliferation differentiation of endometrium and participates in the regulation process of mouse blastodyst implantation.

Dyslipidemia is a risk factor for stroke, and reducing low-density lipoprotein cholesterol (LDL-C) levels can decrease the risk of ischemic stroke. Both statins and PCSK9 inhibitors can effectively reduce LDL-C levels, but some patients cannot tolerate statins, and PCSK9 inhibitors are expensive. Animal experiments have shown that ezetimibe can reduce oxidative stress and endoplasmic reticulum stress response, inhibit cell apoptosis, increase autophagy, and protect nerve tissue by activating AMP activated protein kinase (AMPK) signaling pathway. Clinical studies have shown that ezetimibe can further reduce LDL-C levels on the basis of statin therapy, and reduce the risk of ischemic stroke in patients with acute coronary syndromes, and there is no obvious adverse reaction. However, ezetimibe cannot reduce the incidence of stroke in elderly people who are ≥75 years old, have elevated LDL-C and do not have coronary heart disease. At present, there are few studies on the use of ezetimibe for secondary prevention of ischemic stroke, and its effect needs to be further confirmed by large randomized controlled trials.

Primary nephrotic syndrome refers to one of the common glomerular diseases in children.Glucocorticoid is the first choice in the treatment of nephrotic syndrome, and the using cycle is long.Long term application of glucocorticoids can cause side effects, including Cushing′s disease, hypertension, decreased immune function, growth retardation and eye adverse reactions.Among eye adverse reactions, glaucoma and cataract are more common.Glucocorticoid-induced glaucoma and glucocorticoid-induced cataract are often concealed, and children often cannot accurately express their discomforts.At the same time, some clinicians lack the understanding of the adverse reaction, which is easy to cause missed diagnosis and misdiagnosis.Eventually, irreversible visual impairment occurred.Here, the etiology, pathogenesis, clinical diagnosis, and treatment of glucocorticoid-induced glaucoma and glucocorticoid-induced cataract are elaborated, thus aiming to improve clinicians′ understanding of glucocorticoid ophthalmopathy and the level of clinical diagnosis and treatment.

Objective To investigate the quality of life (QOL) of patients with frequent premature ventricular contractions (PVCs) and the effects of radiofrequency catheter ablation (RFCA) on them.Methods 120 patients with frequent PVCs,100 cases with frequent APCs and 60 healthy persons were assessed by 36-Health survey questionnaire (SF-36) originally.Three months later,the QOL of 48 cases received RFCA and 72 cases with drug therapy were evaluated again by SF-36.Meanwhile,decrease of PVCs were also calculated between these two groups.Results The scores of physiological function and mental health in patients with PVCs were lower than that of cases with APCs,while the score of bodily pain in patients with PVCs was higher (P value:0.0002-0.0380).In addition,the quality of life in patients with frequent PVCs were worse than healthy control(P value:0.0001-0.0915).Three months later,PVCs in 48 cases with frequent PVCs received RFCA were much fewer than that of 72 cases with drug therapy (t=18.8682,P＜0.01),and the quality of life in the former was much better than the latter (F=15.329-39.274,P＜0.01).Conclusion Frequent PVCs can deteriorate the patients' quality of life.RFCA can control or eliminate PVCs in these patients and markedly improve the quality of life of them.

Chemotherapy plays a significant role in cancer therapy,but chemotherapy-induced nausea and vomiting(CINV)common happened and affects patients satisfaction with treatment.With the development ot physiology on emesis,the new antiemetics such as 5-HT3 receptor antagonist(5-HT3RA),Aprepitant and Olanzapine greatly improved the control of CINV,the guidelines for antiemeties were deftned.Research progress of antiemetics was summarized.

Objective To evaluate the prognostic value of enhanced CURB and CURB-65 score in patients with community acquired pneumonia.Methods A retrospective study was conducted among 555 patients with community-acquired pneumonia recruited.According to the patient's 28 day outcome, they were divided into effective group(n=510, 57 cured and 453 improved) and ineffective group(n=45, 30 did not improve and 15 died).The prognosis of the two groups was evaluated using the enhanced CURB and CURB-65 score, the sensitivity and specificity of the two scoring methods were compared.Results Compared with the ineffective group, the age, length of hospital stay and the prevalence of the complications of chronic diseases were significantly lower than the effective group(P<0.05).The score of enhanced CURB and CURB-65 of ineffective group were significantly higher than the effective group(P<0.05).The sensitivity of enhanced CURB score was significantly higher than that of CURB-65 (P<0.05) while its specificity was significantly lower than that of CURB-65(P<0.05).Conclusion The CURB and CURB-65 score in community acquired pneumonia can effectively assess the severity of the disease and thus guide the treatment of patients with community acquired pneumonia.

Urotensin-Ⅱ is a vasoactive 'somatestatin-like' cyclic peptide. Recently, human urotensin-Ⅱ has been cloned and demonstrated to be the most potent vasoconstrictor identified so far. The receptor of urotensin-Ⅱ has now been identified as the orphan receptor GPR 14 . This peptide may influence cardiovarscular homeostasis, pathology and also influence respiratory system, central nervous system and endocrine function.

Objective To develop the method for determining the concert of Ferulic acid. Method The Ferulic acid in Fuke Shiwei Jiaonang capsule was determinined by HPLC. The analytical column was Hanbon Lichrospher (250 mm?4.6 mm?5 ?m) and mobile phase was Acetonitrile-0.085% phosphate acid(17∶83). Ferulic acid was detected at 230 nm. Results In the scope of 0.0204～0.102 ?g, the contents of Ferulic acid had good liner relationship with their peak area (r =0.9999, n =5). The average recovery rate was 98.32%, RSD was 1.64% (n =5). Conclusion The method was rapid, simple and precise.

ObjectiveTo investigate predictive value of matrix metalloproteinase-9 (MMP-9) and Interleukin-6 (IL-6) combined with cervical length in premature delivery.MethodsPlasma samples of 62 pregnant women were detected in this study.Samples were divided into three groups based on pregnancy outcomes.MMP-9 and IL-6 values were determined by ELISA.Cervical length was measured by transvagihal sonography.ResultsThe level of MMP-9 of the pregnant women in the premature delivery group was significantly higher than that of continuous pregnant group and that of normal pregnant women [(47.24 ±6.41) ng/ml,(523.24 ± 62.35) pg/ml vs (20.39 ±7.23 )ng/ml,(219.29 ± 80.23)pg/ml,(32.18 ±5.16)ng/ml,(406.18 ±71.52) pg/ml,P ＜0.01].The level of MMP-9 and IL-6 of the pregnant women in the continuous pregnant group was significantly higher than that of normal pregnant women [(32.18 ±5.16)ng/ml,(406.18 ±71.52) pg/ml vs (20.39 ±7.32) ng/ml,(219.29 ± 80.23) pg/ml,P ＜0.01].Cervical length of the pregnant women in the premature delivery group was significantly shorter than that of normal pregnant women and that of continuous pregnant group [( 15.62 ± 2.31 )mm vs ( 31.05 ± 1.48 )mm,(26.14 ±3.12)mm,P ＜0.01].ConclusionsDetection of level of MMP-9 and IL-6 combined with measurement of cervical length can be used as predicting index of premature delivery.

Objective:To do research on designing the new applied multifunctional integrated tubular pipeline in femoral artery and vein which combined with blood transfusion, fluid infusion, blood sampling, administration, measurement of central venous pressure. Methods: Pipeline is consisted of three parts, which are blood transfusion part and fluid infusion part, including three way switch and three way extended tube. The first way connected with the injection is for blood sampling and doses, the second way connected with the central venous is for measurement of central venous pressure, and the third way connected with the blood transfusion set and fluid infusion set is for blood transfusion and fluid infusion. In a standard thoraco-abdominal combined injury and revivification of 31 beagle, compare the time effect. Results: In the injury and revivification test, the average time cost in 532 times nursing care operation with the multifunctional integrated tubular pipeline is 19+5.1 seconds, the average time cost in 31 times individual nursing care operation of 5 procedure is 80+12 seconds. The multifunctional integrated tubular pipeline can quickly and effectively work on blood transfusion, fluid infusion, blood sampling, administration, measurement of central venous pressure. Conclusion:The multifunctional integrated tubular pipeline in femoral artery and vein saves time and human resources and reduces the infection rate in repeated venous puncture and works quickly and conveniently on animal experiment and acute fatal injury, so it is worthy a further clinical application.