Female ; Humans ; Polycystic Ovary Syndrome ; drug therapy

of apoptosis-related gene.

Objective To explore the sensitivity and constancy of BNP in evaluating the therapeutic effect of congestive heart failure.Methods 80 patients made a definite diagnosis of congestive heart failure were selected and whose cardiac functional grading of NYHA,BNP and left ventricular ejection fraction (LVEF) were determined respectively pre-treatment,one week,one month and three months after-treatment.Statistical analysis on the difference of therapeutic effect was performed for each case.Results No statistical difference was found between the amplitude of BNP and LVEF in one week and on one month, three months pro-treatment by the scheme of captopril combining with metoprolol (P>0.05 ).But between the BNP and the cardiac functional grading,statistica difference was found in the fitst week pro-treament(P<0.05),when on one month and three months after treatment the statistical difference still not found(P>0.05).Conclusion BNP dynamic measuring had the hige sensitivity and constancy in quantitatively analysing the therapeutic effect of congestive heart failure which could be used as a reliable tool for evaluating the therapeutic effect of congestive heart failure.

Forty-five male SD rats were divided into normal group, diabetic control group, and rosiglitazone treatment diabetic group.By the end of 12 weeks, the expressions of pigment epithelium-derived factor(PEDF), matrix metalloproteinase-2 (MMP-2), and transforming growth factor-β1 (TGF-β1) in the kidney were determined by immunohistochemistry and RT-PCR.The results showed that rosiglitazone decreased the increased kidney weight/body weight ratio, serum creatinine, blood ureanitrogen, urinary albumin excretion, triglyceride levels in diabetic rats (all P＜0.01).Rosiglitazone prevented the decreasing of protein expressions of PEDF and MMP-2 and the increasing of protein expression of TGF-β1 (all P＜0.01).PEDF mRNA showed a similar change,suggesting that renoprotection of rosiglitazone on diabetic rats may be mediated through regulating the expressions of PEDF, MMP-2, and TGF-β1.

Tupaiidae has been considered as low primate by most scholars. Focal cerebral ischemia study on tupaiidae has not been reported. Acute focal cerebral ischemic model in tupaiidae was produced by cauterizing the middle cerebral artery. The results indicated that after 15 minutes and 6 hrs occlusion of the middle cerebral artery, the rCBF's in the ischemic cortex were reduced respectively to 36.7% and 39.8% of those before occlusion, and the amplitudes of SFP to 21.83% and 36.78%. After 6 hrs of ischemia, a decrease in ATP content to 5.2 times that of the control, and an increase in AMP to 4.4 times were measured. Calcium and MDA contents in ischemic cerebral cortex increased (P

BACKGROUND:At present, an increasing number of bone graft materials are inactivated using peracetic acid-ethanol solution, but there is no report on whether virus inactivation using peracetic acid-ethanol solution has effects on osteogenic induction of demineralized bone matrix. OBJECTIVE: To explore the effects of virus inactivation using peracetic acid-ethanol solution on the osteogenic activity of demineralized bone matrix. METHODS: Long bones of Sprauge-Dawley rats were selected to make demineralized bone matrix meal. A part of bone meal was placed into gelatin capsules and sterilized by60Coγ irradiation as control group; another part of bone meal was placed into gelatin capsules folowing virus inactivation using peracetic acid-ethanol solution, and then sterilized using60Coγ as experimental group. After that,40 Sprauge-Dawley rats were enroled, and gelatin capsules in the experimental and control groups were respectively implanted into the bilateral psoas muscles of the lumbar spine. At 2, 4, 6, 8 weeks after implantation, the bone grafts were removed for gross and histological observations. RESULTS AND CONCLUSION:At 8 weeks after implantation, the bone mass in the experimental group was not intact in shape with scattered particles, and under light microscope, a few of osteoblasts and new vessels were seen; the bone mass in the control group had hard texture and complete morphology, and under light microscope, mature bone trabeculae wrapped with osteoblasts and new vessels were visible and there were ful of fat cels and bone marrow cells between the bone trabeculae. The number of new microvessels in the experimental group was lower than that in the control group at different time (P < 0.001); at 8 weeks after implantation, the calcium content, alkaline phosphatase content, inorganic phosphorus content and new bone growth rate were all lower in the experimental group than the control group (P < 0.001). These findings indicate that peracetic acid-ethanol for virus inactivation has some negative effects on the osteogenic induction of demineralized bone matrix.

Polycystic ovary syndrome (PCOS) is a disease of dysgenesia and dysmetabolism. Its clinical manifestations include hyperandrogenism, ovulation failure, hyperinsulinemia, elevated serum LH level, obesity and sterility. Histological examination of ovary reveals polycystic changes. It is difficult to carry out clinical research because of its complicated pathogenesis, pathophysiology and multiple biochemical changes and clinical manifestations. It is necessary to study PCOS using animal models. In this article, the authors introduce some methods of establishing animal models of PCOS, such as androgen administration (including testosterone propionate,dehydroepiandrosterone), combination of insulin with HCG, use of estradiol valerate,combination of progestrone with HCG, aromatic cyclase inhibitor method,and so on, and review the effects of traditional Chinese medicine on the intervention of PCOS.

Objective To investigate morphologic, structural and functional characteristics of cardiomyocytes from neonate rats and to set up a desirable technique for isolating and purifying the cardiomyocytes from neonate rats. Methods Using trypsin-digestion, mechanical separation, twice seedings and bromodeoxyuridine (BrdU)-treatment, the cardiomyocytes were isolated and purified from the hearts of neonate rats at 1-7 days after birth. Shapes and spontaneous pulsation of the cells were viewed. The cells were identified with cardiac isoform of tropnin T(cTnT) immunostaining. Ultrastructural features of the cells were examined with in situ transmission electron microscopy. Responses of the cells to adenine and isoprenaline were also examined. Results More than 95% cells isolated from the hearts of neonate rats are cardiomyocytes. The vital cells are more than 95%. Neonate rat cardiomyocytes include short columnar or rhabdoid cells and irregular cells. The most rhabdoid cells from the rats at 1-3 days after birth present the ultrastructural features of immature cardiomyocytes. The rhabdoid cells from the rats at 6-7 days after birth have some ultrastructural features of mature cardiomyocytes. Comparing with the cells at 1-3 days after birth, cTnT expression in the cells is slightly enhanced, the transverse striation was obvious. The irregular cells contain less bundles of myofilaments, the filaments are arranged irregularly. There are a few small cells which are in undifferentiated state. More than 80% cells show spontaneous pulsation at 72 hours after incubation. After treatment with adrenine and isoprenaline, the number of the cells with spontaneous pulsation increases and the intension of spontaneous pulsation is enhanced. The responses of the rhabdoid cells from the rats at 6-7 days after birth to adenine and isoprenaline are much stronger. Conclusion There are two kinds of neonate rat cardiomyocytes. They are different in ultrastructures, spontaneous pulsation and responses to adenine and isoprenaline. The cardiomyocytes from rats at 6-7 days after birth are suitable for experiments in vitro as mature cardiomyocyte. The method set up in this experiment is desirable for culture of neonate rat cardiomyocytes.

Objective　To investigate the alteration and clinical significance of NK cell and T lymphocyte subpopulatien in patients with rectal carcinoma. Methods　 The numbers of NK cell and T lymphocyte in 26 healthy persons and 35 rectal carcinoma patients were detected by 3H-releasing method and FCM. Results　NK cell and CD3, CD4, CD4/CD8 were decreased significantly in patients with rectal carcinoma (P<0.05), which was positively correlated with Dukes staging (P<0.01), and was inereased after redical or palliative operation (P<0.05), but NK cell and CD3, CD4, CD4/CD8 were further decreased in patients with unresectable tumor after operation (P<0.05). Conclusions　The immunity of rectal patients was decreased with the development of the disease, and it will be improved after radical of palliative operation.

The atlantoaxial articulation fracture or dislocation in children is one kind of common damage disease on upper cervical vertebra.Because the children are still in the stage of development,the atlantoaxial articulation ligamentum capsulare and the ligament are relax,so they are easy to be dislocated by external forces or be spontaneous dislocated caused by pathology reasons.The upper cervical vertebra damages on the children are quite different from that on the adults both in anatomical view and in biological term,therefore,the treatment are different from adults either.The treatments can be divided into the conservative ones and the surgery ones,the surgery treatment can also be divided into the posterior atlantoaxial fixed fusion technology,the occipitocervical fixed fusion technology and the odontid screw pexia.No matter using the conservative treatments or the surgical operation treatments,the root cure for the atlantoaxial articulation fracture or dislocation is to re-construct the stability of the articulation.The key point of freatment selection is to grasp the indications of each method.