Objective To assess the correlation between the pregnancy‐associated plasma protein A (PAPP‐A)level with gesta‐tional diabetes mellitus(GDM )in primiparous women .Methods The nonparametric test and Logistic regression analysis were used to retrospectively analyze 168 pregnant women of GDM and 273 normal pregnant women ,including age ,body weight ,PAPP‐A ,freeβ‐human chorionic gonadotropin(fβ‐hCG) .Results The age had significant difference between GDM group and control group(P<0 .01) ,the PAPP‐A concentration and corrected multiple of the median (MoM )of PAPP‐A in the GDM group were significantly lower than those in the control group(P<0 .01) .Conclusion Low level of PAPP‐A during early pregnant stage in primiparas is strongly associated with GDM and can be used as the risk factor of GDM .

Objective To provide morphological data for image diagnosis and mediastinal surgery planning.Methods Cross-sectional images of fresh tissues from the first Chinese visible female human data-set were reviewed and the main structures of the superior mediastinum were confirmed on a section-by-section basis.The positional rule of the main structures in various plane of superior mediastinum was found out,and a few key sectional images were selected and compared with the relative images of CT or MRI,and the relative data on the cross-sectional images were collected.Results The cross-sectional images of the first Chinese visible female human fairly displayed the aortic arch and its 3 large branches,superior vena cava,left and right brachiocephalic veins,azygos vein,pulmonary trunk,trachea,esophagus,thoracic duct and so on,as well as their relationship clearly.Conclusion The first Chinese visible female human dataset can almost provide complete and accurate data.

BACKGROUND:Growth differentiation factor 5 (GDF5) has been shown to play a crucial role in the development of chondrocytes via different regulatory mechanisms.
OBJECTIVE:To explore the effect of GDF5 on the chondrogenic differentiation of adipose-derived stem cel s (ADSCs).
METHODS:ADSCs in passage 4 isolated from Japanese White rabbits were cultured in the medium containing 0 (blank control group), 10, 50, 100, 150 and 200μg/L, respectively. The morphological changes were observed, and the optimal concentration of GDF-5 was screened, and its round coverslips at 1, 2 and 3 weeks of culture were selected to undergo immunocytochemistry, toluidine blue staining and immunofluorescent staining.
RESULTS AND CONCLUSION:The growth of ADSCs was stable in the medium containing 10 and 50μg/L GDF5, while apoptotic cel s appeared after induction with 200μg/L GDF5. In the medium containing 100 and 150μg/L GDF5, some spindle-shaped cel s changed into irregular shape, and became round or oval after 7-10 days of culture. The optimal concentration of GDF5 was 100μg/L. The cel s transfected by 100μg/L GDF5 were positive for type II col agen obviously and with blue-stained nucleus. The transfected cel s were positive for toluidine blue, and metachromatic granules were visible in the cytoplasm. The proteoglycan mRNA expression of the transfected cel s was significantly increased, and reached the highest at 3 weeks. These results suggest that GDF5 promotes the chondrocytic differentiation of ADSCs.

Objective To investigate the effect of nano-?-linolenic acid on the expression of cathepsin B(CB) in mice with viral myocarditis(VM).Methods Eighty male Balb/c mice were randomly divided into 4 groups:control group,myocarditis group,low-dose intervention group and high-dose intervention group,each group had 20 mice.Mice in control group were inoculated introperitoneally with eagle′s solution,every mouse in the last 3 groups was treated with 0.1 mL Coxsackie B3 virus(CVB3) intraperitoneally.Then,mice in low-dose intervention group and high-dose intervention group were treated with 60 mg?kg-1and 180 mg?kg-1 nano-?-linolenic acid solution for 7 days,respectively.Mice in control group and myocarditis group were treated with 9 g?L-1 saline for 7 days.All mice were killed on the 15th day,and the specimens of hearts and serum were conserved.Myocardial histopathology was determined with hematoxylin and eosin stain.The expression levels of myocardial CB mRNA were detected by reverse transcription polymerase chain reaction.Serum CB concentration was examined by enzyme-linked immunosorbent assay.Results The mortality rate was 0,45%,30% and 20% in control group,myocarditis group,low-dose intervention group and high-dose intervention group,respectively;the mortality rate was significantly lower in high-dose intervention group compared with myocarditis group(P0.05).The expression level of CB mRNA and serum CB concentration were markedly higher in myocarditis group than those in control group(Pa

A simple, rapid and sensitive colorimetric reverse-transcription loop-mediated isothermal amplification (RT-LAMP) was developed for rapid detection of Middle East respiratory syndrome coronavirus (MERS-CoV). The method employed six primers that recognized sequences of a nucleocapsid gene for amplification of nucleic acids under isothermal conditions at 63 degrees C for 60 min. Products were detected through a LA-320c Loopamp Turbidimeter (real-time RT-LAMP) or visual inspection of color change by pre-addition of Hydroxynaphthol Blue dye (visual RT-LAMP). Specificity of RT-LAMP was validated by detection of several human coronaviruses and common respiratory viruses. MERS-CoV real-time RT-LAMP had a linear correlation (R2) of 0.995 at 10(3)-10(6) copies. The limit of detection of real-time RT-LAMP, visual RT-LAMP and quantitative real-time PCR was 500, 1000 and 100 copies/reaction, respectively. The established RT-LAMP assay was demonstrated to be a rapid screening tool for MERS-CoV infection, and could be suitable in resource-limited clinical sites and for field studies.
Coronavirus Infections ; virology ; DNA Primers ; genetics ; Humans ; Middle East Respiratory Syndrome Coronavirus ; classification ; genetics ; isolation & purification ; Nucleic Acid Amplification Techniques ; methods ; Reverse Transcription

Objective To establish the model of Chinese visible liver in order to provide the thin-sectional anatomic base at random direction for hepatic ultrasonography. Methods The sectional images collected from the Chinese visible human liver were used to establish the model of visible liver with the aid of visible software and were compared with the corresponding ultrasonic sections of liver. Results The model of Chinese visible liver was achieved and could be sectioned at random direction. Each section could be displayed distinctly,especially the course and distribution of intrahepatic vessels,and had good corresponding relationship in comparison to those of ultrasonic sections of liver. Conclusion Our model of the visible liver can provide the distinct and integrated sectional anatomic data for hepatic ultrasonography.

OBJECTIVETo observe the efficacy of Shenmai Injection (SI) in preventing and treating adverse reation of chemotherapy on advanced non-small cell lung cancer (NSCLC).

METHODSForty-five patients with NSCLC in III b-IV stage were randomly divided into two groups, the control group treated with chemotherapy alone and the treated group with chemotherapy and SI. The therapeutic efficacy were evaluated after 3 treatment cycles.

RESULTSThere was no significant difference in short-term effect between the two groups (P >0.05), while it showed remarkable difference in Karnofsky scoring and body weight (P < 0.05). The treated group showed a better effect than the control group in reducing adverse reaction, such as decrease of leucocyte and hemoglobin (P < 0.05), while in the aspects of improving thrombocytopenia and the reducing occurrence of nausea/vomitting and alleviating injury of liver and kidney function, it only showed the lower value in the treated group, but with no significant difference as compared with the control group (P >0.05).

CONCLUSIONSI could not raise the efficacy of chemotherapy on NSCLC, but improve the quality of life, raise the body weight of patients and alleviate adverse reaction of chemotherapy as myelosuppression so as to improve the tolerance of organism to chemotherapy.

Aged ; Antineoplastic Combined Chemotherapy Protocols ; adverse effects ; therapeutic use ; Carcinoma, Non-Small-Cell Lung ; drug therapy ; Drug Combinations ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Injections, Intravenous ; Leukopenia ; chemically induced ; prevention & control ; Lung Neoplasms ; drug therapy ; Male ; Phytotherapy ; Quality of Life

In this paper, the question about automatic brightness control for x-ray imaging systems based on CCD camera is discussed, and the structure and principle of an auto brightness control loop are analyzed along with the working procedure of the x-ray imaging system. A kind of digital brightness controller about a typical device and the designing idea of the computer brightness intelligent control software is introduced.
Algorithms ; Image Processing, Computer-Assisted ; Radiation Protection ; Radiographic Image Enhancement ; instrumentation ; Radiography ; instrumentation ; X-Ray Intensifying Screens

Objective To reconstruct the Chinese visible human (CVH) dataset with the reconstruction algorithm, the Visualization Toolkit (VTK), and to realize long-distance accessing and alternating operation associated with virtual reality modeling language (VRML) under web condition. Methods 3D structure of the humerus was reconstructed and put out in web condition by applying the methed of capturing data of digitalized visible human. Results The 3D structure of the humerus was successfully reconstructed. These 3D images displayed the anatomical relationships of the structures of the humerus in explore windows with any angle and scale. All reconstructed structures were represented conveniently. Conclusion Based on VTK and VRML, 3D reconstruction of the humerus is realized, which will provide the support and reference for further research on the others structure's visualization of human body, and also for Visualization in Scientific Computing and long-distance teaching in anatomy.

Objective To establish the three-dimensional model of pancreas and its surrounding structures.Methods The three-dimensional pancreas model was established and analyzed by using PC computer platform based on the data set from the first Chinese visible human female.Results The three-dimensional pancreas model,established successfully,could be rotated at any angles and could be sectioned at any orientation.The structures of each section could be displayed clearly.Conclusion Sectional anatomy at any orientation of the pancreas and its surrounding structures can be performed on our pancreas model,which has laid the foundation for virtual surgery of the pancreas.