1.Low efficacy of delthamethrin-treated net against Singapore Aedes aegypti is associated with kdr-type resistance
Pang, S.C ; Chiang, L.P ; Tan, C.H ; Vythilingam, I ; Lam-Phua, S.G ; Ng, L.C.
Tropical Biomedicine 2015;32(1):140-150
There has been a worldwide surge in the number and severity of dengue in the past
decades. In Singapore, relentless vector control efforts have been put in to control the
disease since the 1960’s. Space spraying, fogging, chemical treatment and source reduction
are some commonly used methodologies for controlling its vectors, particularly Aedes aegypti.
Here, as we explored the use of a commercially available delthamethrin-treated net as an
alternative strategy and the efficacy of the treated net was found to be limited. Through
bioassays and molecular studies, the failure of the treated net to render high mortality rate
was found to be associated with the knockdown resistance (kdr) mutation. This is the first
report of kdr- mutations in Singapore’s Ae. aegypti. At least one point mutation, either
homozygous or heterozygous, at amino acid residue V1016G of DIIS6 or F1269C of DIIIS6
was detected in 93% of field strains of Ae. aegypti. Various permutations of wild type and
mutant amino acids of the four alleles were found to result in varying degree of survival rate
among local field Ae. aegypti when exposed to the deltamethrin treated net. Together with
the association of higher survival rate with the presence of both V1016G and F1269C, the
data suggest the role of these mutations in the resistance to the deltamethrin. The high
prevalence of these mutations were confirmed in a country wide survey where 70% and 72%
of the 201 Ae. aegypti analysed possessed the mutations at residues 1016 and 1269 respectively.
The highest mutated frequency combination was found to be heterozygous alleles (VG/FC) at
both residues 1016 and 1269 (37.8%), followed by homozygous mutation at allele 1269 (24.4%)
and homozygous mutation at allele 1016 (22.9%). The kdr- type of resistance among the
vector is likely to undermine the effectiveness of pyrethroids treated materials against these
mosquitoes.
2.Seizure Induced Bilateral Cervical Facet Dislocation: A Case Report
C.H. Foo ; MH. Tan ; Faruk NA ; Y.Y. Teo ; B.B. Tan ; Zaki MA ; C.C. Wong
Malaysian Orthopaedic Journal 2019;13(Supplement A):213-