Background: Caesarean section is a common surgical practice in gynecology. The rate of caesarean section is on the increase not only in Vietnam but also worldwide.\r\n', u'Objectives: To determine the rates of the caesarean section and related factors in mothers delivering from Jan 1st 2007 to Mar 31st 2007. Subjects and Methods: The cross-sectional study was carried out on 423 women (219 in Hoan Kiem district and 204 in Gia Lam district) from April to September 2007.\r\n', u'Results:Caesarean section rates in Hoan Kiem and Gia Lam district were 37.9% and 22.1%, respectively; total caesarean section rate was 30.3%. About 96-98% women who had history of caesarean delivery were going to give birth by caesarean sections. Caesarean section for non-medical reasons accounted for 14.6% of the women. There were 14% of mothers choosing caesarean sections for selecting the good day or the good hour and 16.7% for the impact of their family. \r\n', u'Conclusion: Caesarean section rate in this study were quite high, previous caesarean sections in patient history were one of the main reasons, non-medical caesarean reasons were not low in rate, the age of the women was one of the factors associated with caesarean sections. \r\n', u'\r\n', u'
Caesarean sections ; real situation ; related factor

Background: Domestic-related violence against women appears in every country, culture and socioeconomic classes. Yen Phong district of Bac Ninh province is in the economic transformation process from agricultural to industrial and service economic structure. The domestic-related violence trend is increasing, but there are no study on this situation. Objectives: The study was conducted to investigate the situation of domestic violence against women in Yen Phong district, Bac Ninh province. Subjects and method: The study used cross-sectional descriptive design. Participants were married women who were less than 50 years old. The participants were interviewed with structured questionnaires. The group involved the men who have a history of violent activities against women and the women who suffered from violence was thoroughly interviewed and discussed. Results: A total of 452 women had participated in the study. Mean age of participants was 34.6 years old (ranging from 19 to 49 years old). About half of participants have never suffered from domestic violence. The violence in the last 12 months was lower, affected to one out of every 5 women. The mental violence accounted for highest rate, followed by physical and sexual violence (29.2%, 28.3% and 10.4%, respectively). Sharing economy and household responsibility, gambling habit of husbands and talkative habit of wives were main reasons of violence. The consequences of violence were mainly related to mental and social factors. Some women suffered from mental, physical and sexual health consequences (73.2%, 20.5%, and 13.7%, respectively). Conclusion: In order to increase the awareness on domestic violence, it requires improve access to information on gender violence for both men and women.
Gender violence ; Rural women

Background: Domestic violence is a common occurrance in every country, culture and all levels of society, and about 20%-50% of women worldwide are victims of domestic violence. In Vietnam, domestic violence had broad range of influences and has serious impacts. Objectives: To identify the factors influencing domestic violence in Yen Phong district, Bac Ninh province. Subjects and method: A cross-sectional study was conducted in 2006 on 452 married women aged 19 to 45 years, with structured questionnaire and in-depth interviews and focus group discussion. Results and conclusion: the lifetime prevalence of domestic violence was 42% and the prevalence in the past 12 month was 22.6%. The most frequent domestic violence was mental, followed by physical and sexual violence. The first 15 years of marriage was the most turbulent period of violence. There were significant relationships between lifetime violence and the education, occupation, economic status, but there was no relationship for violence in the past 12 months. The women, whose husbands were drunk, gambling and unfaithful, were more likely to be facing domestic violence than the others. The acceptance of violence by women was significantly related to violence. Those who perceived the violence by husbands were very severe then were less likely to be faced by violence than the other. In order to prevent the domestic violence, it is necessary to promote the health education, improving the awareness of women and men on gender equity and equality.
Domestic violence ; Gender equity

Background: DNA biosensor is based on micro-nano technologies aimed at developing a rapid diagnostic device of infectious diseases and diseases related to genetic change. Biosensors are compact size, high sensitivity and low cost\r\n', u'Objectives: To evaluate effect of biosensor in detecting specific gene fragments of Herpes Simplex virus type 1 and 2 (HSV)\r\n', u'Subjects and method: The electrical signals were recognized by means of transducer and from electrochemical detection of the hybridization between the probe 5\ufffd?AT CAC CGA CCC GGA GAG GGA C-3\ufffd?which were covalently immobilised onto the surface of micro electrodessensors in 3-aminopropyltri-ethoxysilance (APTS)-the conducting polymer matrix and the target (specific DNA sequences of HSV in the sample.\r\n', u'Results: The DNA sensor offers a very high sensitivity, a fast response time, less than 1 min with the DNA target concentration up to 1nM in aqueous media at room temperature.However, in order to detect target DNA in the real samples, samples must be extracted DNA, denatured DNA sequences from a double fiber to single fiber. The measurement should be done soon\r\n', u'Conclusion: The results show a large promise to develop quickly DNA sensors for widely application in bio-medical research \r\n', u'\r\n', u'
Biosensing Techniques ; CNBP protein ; human ;

OBJECTIVE: Sperm cryopreservation has been widely used in assisted reproductive technology, as it offers great potential for the treatment of some types of male infertility. However, cryopreservation may result in changes in membrane lipid composition and acrosome status, as well as reductions in sperm motility and viability. This study aimed to evaluate sperm DNA fragmentation damage caused by conventional freezing using the sperm chromatin dispersion test. METHODS: In total, 120 fresh human semen samples were frozen by conventional methods, using SpermFreeze Solution as a cryoprotectant. Routine semen analysis and a Halosperm test (using the Halosperm kit) were performed on each sample before freezing and after thawing. Semen parameters and sperm DNA fragmentation were compared between these groups. RESULTS: There was a significant decrease in sperm progressive motility, viability, and normal morphology after conventional freezing (32.78%, 79.58%, and 3.87% vs. 16%, 55.99%, and 2.55%, respectively). The sperm head, midpiece, and tail defect rate increased slightly after freezing. Furthermore, the DNA fragmentation index (DFI) was significantly higher after thawing than before freezing (19.21% prior to freezing vs. 22.23% after thawing). Significant increases in the DFI after cryopreservation were observed in samples with both normal and abnormal motility and morphology, as well as in those with normal viability. CONCLUSION: Conventional freezing seems to damage some sperm parameters, in particular causing a reduction in sperm DNA integrity.
Acrosome ; Chromatin ; Cryopreservation ; DNA Fragmentation ; DNA ; Freezing ; Humans ; Infertility, Male ; Male ; Membranes ; Reproductive Techniques, Assisted ; Semen ; Semen Analysis ; Sperm Head ; Sperm Motility ; Spermatozoa ; Tail

OBJECTIVE: In frozen and thawed embryos, the zona pellucida (ZP) can be damaged due to hardening. Laser-assisted hatching (LAH) of embryos can increase the pregnancy rate. This study compared thinning and drilling of the ZP before frozen embryo transfer (FET). METHODS: Patients were randomly allocated into two groups for LAH using thinning or drilling on day 2 after thawing. Twenty-five percent of the ZP circumference and 50% of the ZP thickness was removed in the thinning group, and a hole 40 µm in diameter was made in the drilling group. RESULTS: A total of 171 in vitro fertilization/intracytoplasmic sperm injection FET cycles, including 85 cycles with drilling LAH and 86 cycles with thinning LAH, were carried out. The thinning group had a similar β-human chorionic gonadotropin-positive rate (38.4% vs. 29.4%), implantation rate (16.5% vs. 14.4%), clinical pregnancy rate (36.0% vs. 25.9%), miscarriage rate (5.8% vs. 2.4%), ongoing pregnancy rate (30.2% vs. 23.5%), and multiple pregnancy rate (7.0% vs. 10.6%) to the drilling LAH group. There were no significant differences in pregnancy outcomes between subgroups defined based on age (older or younger than 35 years) or ZP thickness (greater or less than 17 µm) according to the LAH method. CONCLUSION: The present study demonstrated that partial ZP thinning or drilling resulted in similar outcomes in implantation and pregnancy rates using thawed embryos, irrespective of women's age or ZP thickness.
Abortion, Spontaneous ; Chorion ; Embryo Transfer* ; Embryonic Structures* ; Female ; Humans ; In Vitro Techniques ; Methods ; Pregnancy ; Pregnancy Outcome ; Pregnancy Rate ; Pregnancy, Multiple ; Reproductive Techniques, Assisted ; Spermatozoa ; Zona Pellucida

BACKGROUND/OBJECTIVES: Although children of ethnic minority groups are experiencing a transition from a predominance of undernutrition to overnutrition, there is little evidence of a dual-malnutrition burden. Therefore, this study examined the double burden of malnutrition among school-aged ethnic minority children living in mountainous areas and its association with their diets. SUBJECTS/METHODS: A cross-sectional study was conducted from September 2019 to March 2020 in 3 mountainous areas of Northern Vietnam among 1,556 ethnic minority schoolaged children. The prevalence of under-nutrition (stunting and thinness) and over-nutrition (overweight and obesity) were measured using the WHO 2006 child growth standards (height-for-age and BMI-for-age Z-score). Nutritional practices were evaluated by the frequency of food consumption based on a 4-level scale. RESULTS: The percentage of children with stunting and thinness were 14.0% and 5.4%, respectively, while the figure for overweight/obesity was 9.4%. The factors positively associated with stunting were living in a family with more than 2 children or being Muong/ other ethnicities compared to the Tay ethnicity. Children who consumed fish/shrimps/crabs or milk weekly/daily were less likely to be undernourished compared to those who never consumed these foods. By contrast, children who never consumed foods rich in vitamin A precursors and vitamin A and fruit or consumed daily snacks/junk food were more likely to be overweight/obese. CONCLUSIONS Undernutrition remains a common issue among school-aged children and adolescents of ethnic minority groups, while over-nutrition exists simultaneously. Public health nutrition programs promoting adequate diets and positive lifestyle changes related to nutrition are essential to tackle the double burden of malnutrition among ethnic minority children.

As the associations of sperm DNA fragmentation with morphology have not been examined in detail, this study aimed to investigate the relationship between abnormalities of morphological details and DNA integrity in human sperm. Methods: In this cross-sectional study, men from infertile couples were enrolled at Hue Center for Reproductive Endocrinology and Infertility, Vietnam. Conventional semen parameters, including morphological details, were analyzed following the World Health Organization 2010 criteria. Sperm DNA fragmentation was evaluated using a sperm chromatin dispersion assay. The relationships and correlations between semen parameters, sperm morphology, and the type of halosperm and the DNA fragmentation index (DFI) were analyzed. Results: Among 130 men in infertile couples, statistically significant differences were not found in the sperm halo type between the normal and abnormal sperm morphology groups. The percentage of round-head spermatozoa was higher in the DFI >15% group (16.98%±12.50%) than in the DFI ≤15% group (13.13% ±8.82%), higher values for amorphous heads were found in the DFI >15% group, and lower values for tapered heads were observed in the DFI ≤15% group; however, these differences were not statistically significant. Small-halo sperm and the DFI were positively correlated with round-head sperm (r=0.243, p=0.005 and r=0.197, p=0.025, respectively). Conclusion: The rate of general sperm morphological abnormalities in semen analysis was not related to sperm DNA integrity. However, round sperm heads were closely associated with sperm DNA fragmentation.

Objective: This study aimed to compare the efficacy of physiological intracytoplasmic sperm injection (PICSI) and intracytoplasmic sperm injection (ICSI) in terms of the fertilization rate and embryo quality using sibling oocyte cycles. Methods: This prospective, cross-sectional study collected data from 76 couples who underwent their first cycle at the Hue Center for Reproductive Endocrinology and Infertility, Vietnam, between May 2019 and November 2021. The inclusion criteria were cycles with at least eight oocytes and a sperm concentration of 5×106/mL. Sperm parameters, sperm DNA fragmentation (SDF), fertilization, and the quality of cleavage-stage embryos on day 2 and blastocysts on day 5 were examined. Results: From 76 ICSI cycles, 1,196 metaphase II (MII) oocytes were retrieved, half of which were randomly allocated to either the PICSI (n=592) or ICSI (n=604) treatment group. The results showed no significant difference between the two groups in terms of fertilization (72.80% vs. 75.33%, p=0.32), day 2 cleavage rate (95.13% vs. 96.04%, p=0.51), blastulation rate (52.68% vs. 57.89%), and high-quality blastocyst rate (26.10% vs. 31.13%, p=0.13). However, in cases where SDF was low, 59 cycles consisting of 913 MII oocytes produced a considerably higher blastulation rate with PICSI than with ICSI (50.49% vs. 35.65%, p=0.00). There were no significant differences between the pregnancy outcomes of the PICSI and ICSI embryo groups following embryo transfer. Conclusion Using variable sperm quality provided no benefit for PICSI versus ICSI in terms of embryo outcomes. When SDF is low, PICSI appears to be able to produce more blastocysts.

Background: With the development of HIV/AIDS pandemic in community, amount of HIV/AIDS people more and more increase in prison. Objectives: Determine the rate of HIV/AIDS infection of prisoners at Binh Dien prison. Learning about related elements to HIV/AIDS infection. Subjects and method: Prisoners at Binh Dien prison. Method: 492 prisoners were chosen, interviewed directly and taken blood samples to HIV test. Using cross-sectional study on accidental samples. Techniques were used in this study: SERODIA-HIV and ELISA technique. Results: The rate of current HIV/AIDS infections was 21,74%, some HIV/AIDS related factors in the prison included sex, religion, income source, marrital status, common knowledge of HIV/AIDS, drug addiction history, needle sharing, sexually transmitted disease history, body tatoos and forskin inserted with metal balls, alcohol and beer drinking history, some factors such as: age and educational level were not associated with HIV/AIDS infection in prison. Conclusion: Strengthen interventional activities in prison to prevent HIV/AIDS spread between prisoners. To prisoners who were not infected HIV/AIDS: strengthen educational communication to they may prevent themselves from HIV/AIDS infection behaviors. To HIV/AIDS prisoners: Need to educate and consult especially to they have right behaviors, prevent HIV/AIDS spread for the others while they were in prison as well as they return to society.
HIV Infections/ blood ; complications ; epidemiology ;