No Abstract Available.
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We introduced a compulsory medical ethics course for first-year medical students so that they would understand the basis of bioethics thinking and acquire the basic capacity to solve patients' clinical problems. The course consists of 14 school hours, of which 11 were for group discussion of 2 clinical cases and 3 were for whole-class lectures on various ethical issues. Identical, short ethics tests were given on the first and last days of the course. The acceptance level was evaluated on the basis of the score of the second test, reports submitted after group discussions, and class attendance. The scores of the second test correlated with the results of reports of the second clinical case but not with those of the first clinical case. Logistic regression analysis indicated that factors contributing to the acceptance level were the scores of the second test and the attendance rate, which was an independent contributing factor. Furthermore, the scores of the second test, but not of the first test, correlated with the acceptance level. These results indicate that this ethics course is useful for increasing students' thinking about ethical issues.

The quantitation of serum tocilizumab using liquid chromatography tandem-mass spectrometry(LC-MS/MS)method has not been widely applied in clinical settings because of its time-consuming and costly sample pretreatments.The present study aimed to develop a validated LC-MS/MS method for detecting serum tocilizumab by utilizing immobilized trypsin without an immunoglobulin G purification step and evaluate its applicability in the treatment of rheumatoid arthritis(RA)patients administered intrave-nously or subcutaneously with tocilizumab.The tocilizumab-derived signature peptide was deciphered using a nano-LC system coupled to a hybrid quadrupole-orbitrap mass spectrometer.The serum tocili-zumab was rapidly digested by immobilized trypsin for 30 min.The chromatographic peak of the signature peptide and that of the internal standard were separated from the serum digests for a total run time of 15 min.The calibration curve of serum tocilizumab concentration was linear with a range of 2-200 μg/mL.The intra-and inter-day accuracy and relative standard deviation(RSD)were 90.7％-109.4％and＜10％,respectively.The serum tocilizumab concentrations in the RA patients receiving intravenous and subcutaneous injections were 5.8-28.9 and 2.4-63.5 pg/mL,respectively.The serum tocilizumab concentrations using the current method positively correlated with those using the enzyme-linked immunosorbent assay,although a systematic error was observed between these methods.In conclu-sion,a validated LC-MS/MS method with minimal sample pretreatments for monitoring serum tocili-zumab concentrations in RA patients was developed.