1.Overexpression of ephrinB2 in canine periodontal ligament stem cells regulates osteogenic differentiation
Yumiao Liu ; Jinxin Yang ; Shaoyue Zhu ; Yulin Ma ; Hanping Xia ; Meng Zhou ; Shulei Yang ; Taining Huang ; Kexin Ding ; Zongxiang Liu
Acta Universitatis Medicinalis Anhui 2023;58(3):423-428
Objective:
Investigate osteogenic differentiation of canine periodontal ligament stem cells ( cPDLSCs) via over-expression ephrinB2 in cPDLSCs．
Methods :
cPDLSCs were isolated from the premolars and molars of Beagle．After transfected with EfnB2-GFP-Bsd and GFP-Bsd empty Vector，cPDLSCs were induced to osteogenic differentiation．Western blot was used to invest the expression of ephrinB2 protein．The effect of osteogenic differentiation of EfnB2-cPDLSCs and Vector-cPDLSCs were analyzed by RT-PCR , CCK-8，Alizarin-red S staining and ALP.
Results:
There was no significant difference in cell proliferation between EfnB2-cPDLSCs and Vector-cPDLSCs．While EfnB2-cPDLSCs displayed an enhanced ALP activity and more prominent mineralized nodules compared with Vector-cPDLSCs．The odonto-/ osteogenic genes in EfnB2-cPDLSCs were also highly enhanced．
Conclusion
The results of our study indicated that ephrinB2 gene-transfected cPDLSCs showed enhanced osteogenic differentiation.
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