Objectives:To study the localization of dehydroepiandrosferone (DHEA) in rat gastric epithelial cells.Methods:The immunohistochemical SABC was used.Results:The parietal cells in gastric epithelial cells appeared DHEA positive immune reaction.The positive substance was distributed in the cytoplasm and negative in the nuclei.Conclusions:Parietal cells had DHEA,which might play an important role in the regulation of digestive function.

Objective To observe the effect of Yinhuang freeze-dried powder injection on nervous system of rats and mice,and to probe its safety.Methods After injected Yinghuang freeze-dried powder injection nerve system and general behavior of rat or mouse were observed.Results Yinghuang freeze-dried powder didn’t affect rat’s or mouse’s general behavior,but had certain central nevous system suppression function in rats and mice.Conclusion Yinghuang freeze-dried powder have certain central nevous system suppression function in rats and mice.

Objective To observe the rat chronic toxicity induced by Yinghuang freeze-dried powder injection. Methods The rats were given Yinghuang freeze-dried powder injection for 35 days with the doses of 166.5, 82.25, 41.12 mg/kg and for 15 days of stopping administration. The index sign of hematology and biochemistry were determined, and the pathological examination were carried out. Results The rat were given Yinghuang freeze-dried powder injection for 35 days and for 15 days of stopping administration. Mostly index sign of hematology and biochemistry had no significant difference by comparing with the control. There exceptional index sign of exceptional dosage group have significant difference by comparing with the control. But these exceptional index sign fluctuated in normal range in the laboratory. The pathological examination did not discover significant pathological changes related to drug toxicity. Conclusion Yinghuang freeze-dried powder injection has no significant toxicity for long-term administration on rats. It infers that the drawing up doses in clinic will be safe.

Objective To investigate the effects of the serum contained Shuluo sterile powder for injection (SLI) on injury induced by anoxia and hypoglycemia in cultured PC12 cells. Methods Cultured PC12 cells were treated with Na2S2O4 and Earle′ s liquid with glucose free to induce models of anoxia and hypoglycemia. The protective effects of the serum contained SLI on the two kinds of models were evaluated by the activity of lactate dehydrogenate (LDH) and staining with MTT assay. Results The serum contained SLI counteracted the injury induced by anoxia and hypoglycemia to various degrees and prevent the efflux of LDH. Conclusion The serum contained SLI can protect PC12 cells from injury induced by anoxia and hypoglycemia.

Objective To observe the influence of bony extracts of Myospalox fontanierii(MF)on immune function in rats with hypersensitive reactive inflammation induced by Freund's complete adjuvant(FCA).Methods Levels of lymphocyte transferring factor(TF)and interleukin-1(IL-1)were detected by immunological methods.Results Extracts of MF reduced the levels of IL-1 and TF in rats with hypersensitive reactive inflammation induced by FCA;it could also depressed the lymphocytic transformation rate.Conclusion Extracts of MF exert inhibitory effect on rats with hypersensitive reactive inflammation induced by FCA and its mechanism may be related with immunosuppressive action and the inhibition of IL-1 formation.

AIM: To study Qinzhi Capsule(Radix Scutellaniae,Fructus Gardeniae) against virus and bacteria. METHODS: The mice were infected separately by Ⅰ、Ⅱ type flu virus,staphylococcus aureus or pneumococcus in the experiment.The protective action of Qinzhi Capsule on them was observed in vivo.and detemined mininum inhibition concettation(MIC). RESULTS: Qinzhi Capsule could prolong markedly the survival periods of mice(P

AIM: To study the anti-inflammatory,analgesic and antipyretic effects of Qinzhi Capsule(Radix Scutellariae,Fructus Gardeniae,etc). METHODS: Qinzhi Capsule was taken orally.The experimental methods were as follow: abdomen capillary permeability in mouse induced by actetic acid,mouse ear swell induced by dimethylbenzene,mouse pain threshold induced by heat stimulation,animal fever induced by yeast to rat and endotoxin to rabbit,et al. RESULTS: Qinzhi Capsule could inhibit markedly the increase in abdomen capillary permeability in mouse induced by acetic acid(P

Objective To investigate the effect of Shuluo Injection on cerebral water volume,cerebral index ,LDH,NOS and SOD in the brain.Methods The model of cerebral ischemia-reperfusion in mice is set up by separating the bilateral common carotid artery (CCA) of the mice,blocking the blood stream by straining the thread and by reperfusion loosing the thread. After 3 times of ischemia-reperfusion,brain samples were prepared to observe the cerebral injury. Results High-dose Shuluo Injection significantly reduced the cerebral water volume and decreased the cerebral index and the activity of LDH and NOS(P

Objective To explore the mechanism of Huagan Jiedu decoction in treatment of chronic hepatitis B by testing the influence of the decoction on Serum IL-2, IL-4 and IFN-γ of rat models of chronic hepatic injury. Methods The model of Chronic hepatic injury was established by subcutaneously injecting 40% CCl4-olive oil solution in rats according to the ratio of 3ml per kg. of body weight. 60 healthy rats were divided randomly into a normal group, a model group, a low dose of Huagan Jiedu decoction group, a large dose of Huagan Jiedu decoction group, and a control group (Qingre Jiedu tablet group), 15 cases in each group. The Serum IL-2, IL-4 and IFN-γ were detected in each group separately. Results Compared with the normal group, the Serum IL-2 and IFN-γ were decreased (t=2.401, 2.337, P=0.0349, 0.0378) and the Serum IL-4 was increased (t=2.896, P=0.00861 ) in the model group; Compared with the model group, the Serum IL-2 and IFN-γ were increased (t=2.417, 2.344, P=0.0341, 0.0372, P＜0.05; t=3.114, 2.988, P=0.0052, 0.0068) in the large Huagan Jiedu decoction group and the control group, while the Serum IFN-γ was increased (t=3.049, P=0.0062) in the low dose of Huagan Jiedu decoction group and the Serum IL-4 was decreased (t=3.102, 3.017, 2.979, P=0.0061, 0.0065, 0.0069)in both Huagan Jiedu decoction groups and the control group. Conclusion Large dose of Huagan Jiedu decoction has sound therapeutic effects on rats with chronic hepatic injury by decreaseing the content of the Serum IL-4 and increasing IL-2 and IFN-γ, and adjusting th1/th2 balance.

AIM: To observe the effect of Qingluo Tongbi Granule(QLTBG)——containing serum on the proliferation of osteoblasts(OB) and osteoclasts(OC),the activity of AKP of OB. METHODS: (1) OB were separated from the skull of SD rats aged 1 d and OC from the thighbone and shinbone of SD rats aged 5 d.(2) The proliferation of OB and OC was detected by MTT method,and the activity of AKP of OB was examined by diazol method. RESULTS: The proliferation capability of OB was strengthened markedly by the rat sera containing 7.2,14.4 g/kg QLTBG,which could reinforce the activity of AKP obviously as well,while the proliferation of OB was inhibited remarkably by the rat sera containing 3.6,7.2,14.4 g/kg QLT. CONCLUSION: The sera containing QLTBG could enhance the proliferation of OB and the activity of AKP,restrain the proliferation of OC simultaneously.