Biosafety cabinets(BSC)is the primary containment barrier.It is very important to choose suitable control method for the protection ability of biosafety cabinet.This paper briefly introduces the key points and several control methods as well as the different characteristics of BSC control.

Biological safety is an important international issue. For this reason, WHO published Laboratory Biosafety Manual in 1983, 2003 and 2005. According to the concepts of Biological safety, countries all over the world have developed and established their own national codes of practice for the safe handling of pathogenic microorganism for clinical, studying and epidemiological purposes. In BSL-4 laboratories, individual biosecurity is very important for staffs when they are faced with dangerous level-4 pathogens. In this paper, individual protective equipment and correlative technical requirements for BSL-4 laboratories are introduced.

Objective To investigate the predictive value of platelet parameters on distant metastasis of different clinical stages of non‐small cell lung cancer (NSCLC) .Methods A retrospective analysis was performed on the inpatients with lung cancer in our hospital from April 2013 to January 2014 .The parameters of platelet count (PLT ) ,mean platelet volume (MPV) ,platelet distribu‐tion width (PDW) ,platelet ‐large cell ratio(P‐LCR) ,platelet hematocrit (PCT ) ,etc .were detected respectively .Results The re‐sults showed that PLT ,MPV ,P‐LCR and PCT in the lung cancer group were higher than those in the benign lung tumor group and the healthy control group ,the differences were statistically significant (P< 0 .01) ,while PDW had no statistically significant differ‐ence among these groups(P> 0 .05) .With the TNM stage increase ,the increased platelet proportion in the stage Ⅲ and Ⅳ was sig‐nificantly higher than that in the stage Ⅰ and Ⅱ (32 .35% ,42 .85% vs .19 .35% ,22 .85% ,P< 0 .01) .The platelet five parameters in the stage Ⅳ had statistical difference compared with those in the stage Ⅰ and Ⅱ (P< 0 .05) ;but PDW and P‐LCR in the stage Ⅲhad no statistical difference compared with those in the stage Ⅰ and Ⅱ (P> 0 .05) ;PLT ,MPV and PCT in the stage Ⅳ had statisti‐cal difference compared with those in the stage Ⅲ (P< 0 .05) .Conclusion The patients with lung cancer often stimulate the bone marrow megakaryocytes to produce platelets by a particular mechanism .As the staging increase ,the proportion of increased platelet is more obvious .Continuously and dynamically observing platelet parameters is conducive to judge the development and prognosis of the disease .

To explore primarily the reconstruction of biological tissue engineered blood vessel in vitro, the decellularised scaffolds were obtained from swine common carotid arteries by enzyme digestion, and vascular smooth muscle cells (VSMCs) were isolated from canine thoracic aorta and subcultured and purified. The VSMCs were seeded into the inner surface of scaffolds and they were cocultured in vitro for 4 weeks. Histological staining and transmission electron microscopy were used to observe the growth of canine VSMCs on swine decellularized arterial scaffolds. The results showed the seeded VSMCs grew well on the decellularized scaffold throughout the duration of 4 weeks, which suggests that the method in this article is practicable for reconstructing complete biological tissue engineering blood vessel.
Animals ; Arteries ; Blood Vessel Prosthesis ; Cells, Cultured ; Coculture Techniques ; Dogs ; Microscopy, Electron, Transmission ; Muscle, Smooth, Vascular ; cytology ; Myocytes, Smooth Muscle ; cytology ; Swine ; Tissue Engineering ; Tissue Scaffolds