This study was conducted on 82 nonsmoking healthy young volunteers divided into two groups. The GB37 group consisted of 39 people who were subjected to stimulation of the Guangming (GB37), and the control group consisted of 43 people who were not so stimulated. Blood-flow velocity and pulsatility index (PI) in the central retinal artery (CRA) of the right eye were measured using Color Doppler imaging. Blood pressure and heart rate were also measured at the same time. The type of stimulus was acupuncture applied on the right GB37 with the needle retained for 15min. Seven measurements were made at intervals of 7.5min during a 45-minute period while the subject was in the sitting position. The GB37 group was first measured just before stimulation and observed for 30 min after the needle was removed. There were significant differences in blood-flow velocity and PI between the two groups. In the GB37 group, blood-flow velocity in the CRA increased remarkably and PI decreased remarkably. These values scarcely changed in the control group. There were no significant differences in blood pressure or heart rate between the two groups. These results suggest that acupuncture stimulus on GB37 changes the circulation of CRA by a mechanism other than blood pressure, resulting in an increase in retinal blood-flow volume.

We previously reported the possibility that acupuncture stimulation to the Guangming (GB37) increased retinal blood-flow volume. In this study, we examined whether this reaction was peculiar to GB37 by measuring the blood-flow velocity and pulsatility index (PI) of the central retinal artery (CRA) with Color Doppler imaging. The points to be stimulated were the Waiqiu (GB36), GB37, the Yangfu (GB38) or the non-meridian point on the outside of the crus. Acupuncture stimulus was applied to one point on the right side with a needle for 15min. Seven measurements were made at intervals of 7.5min during a 45-minute period while the subject (control group n=35, stimulus group n=89) was in the sitting position. Patterns of the change in blood-flow velocity and PI with time differed significantly between the five groups. An increase in retinal blood-flow volume occurred in the GB37 group only suggesting there was a peculiarity related to the meridian point. The reaction patterns of the right and left eyes were not significantly different. Blood pressure and heart rate exhibited no significant differences either. These results suggest the relevance of choosing GB37 for improving or maintaining the retinal blood-flow volume.

[Objective]We researched the effect of five distal acupuncture points that is used for a treatment of eye disorders. [Method]This study was conducted on healthy non-smoking volunteers. We observed change at the time of the rest (Study 1, the control group n=65), and we observed effects of ocular circulation by acupuncture stimulation (Study 2, the stimulus group n=118). Stimulus groups were divided into five groups that are according to the acupoint stimulated as following:GB20 (Fuchi) group, LI4 (Gokoku) group, BL18 (Kanyu) group, GB37 (Komei) group and LI11 (Kyokuchi) group. Blood-flow velocity and pulsatility index (PI) in the central retinal artery (CRA) of the eye were measured using color Doppler imaging. An acupuncture needle was retained for 15min at the selected point. Dates were obtained at intervals of 7.5 min for a total of seven times during a 45-minute period while the subject was in the sitting position. [Results]In the control group showed no significant changeas for all cases of blood-flow velocity and PI. In groups with the stimulus showed that blood-flow velocity in the CRA was increased significantly and PI was decreased significantly. Blood pressure and heart rate scarcely changed in two groups.[Conclusion]These results suggest that acupuncture on the distal acupuncture points that is used for a treatment of eye disorders changes the circulation of CRA. The effect on the ocular circulation was greater in GB37 or in LI4, and was comparatively smaller in GB20.

Objective: Very few studies have been reported on the specific functions of individual meridians and acupuncture points. We examined the functional relations between “the gallbladder Meredian and gallbladder” using the gallbladder's form as an index, in older to identify the specific functions of individual meridians and acupuncture points.
Subjects and Methods: This study was made on five healthy male adult volunteers who fasted during the experiment day.
The gallbladder was selected as the target organ and its form was measured with an ultrasonic diagnostic apparatus (Toshiba SSA-90A). The images of the gallbladder form were taken at the pointwhen the major axis of the cross-sectional area of the gallbladder was maxium. The cross-sectional area of the gallbladder was measured with an image analyzer. The gallbladder form was measured 15 minutes after the subject had lied on his back. Images were taken at intervals of 2 to 5 minutes 10 minutes before stimulation and for 30 minutes during and after stimulation. Acupuncture stimulation was given at the points of C34. Dannang, G36, G37, G40, and G44 on the right side of the body. After getting the degi, 1 minute of sparrow picking needle technique and 1 minute of leaving needle technique were repeated three times.
Results: 1) Stimulation of the G34, G36, G37, and G44 gave no effects on the gallbladder form. 2) Stimulation of the Dannang (EX) produced contraction of the gallbladder. 3) Stimulation of the G40 produced distension of the gallbladder. From the above results, we found that there two types of acupuncture points; one causes contraction of the gallbladder, and the other, distension of the gallbladder. This suggests that individual acupuncture points have specific functions.

To investigate the effect of acupuncture treatment combined with extreme infra-red rays radiation, we measured both skin surface and deep temperature at the stimulated area and peripheral site. The subjects were seven healthy volunteers with no problems involving the skin or autonomic nervous system. Acupuncture stimulation was performed on the Subject's back in combination with extreme infra-red rays radiation. The skin surface and deep temperature were measured at both the stimulated area and peripheral site (sole of the foot) using a thermistor temperature sensor and deep tissue thermometer during stimulation. We also measured temperature at those sites during extreme infrared rays radiation without acupuncture stimulation as a control study. Acupuncture stimulation combined with extreme infra-red rays radiation on the subject's back increases both surface and deep temperature at both the stimulated area and the peripheral site (sole of the foot), while the radiation alone had no effect on the temperature at the peripheral site. We suggested that acupuncture treatment combined with extreme infra-red rays radiation was useful to increase skin surface and deep temperature not only at the stimulated area but also at the peripheral site.

The purpose of this study is to examine the validity of muscle fatigue evaluation using maximum voluntary torque (MVT), and to identify the dependence of individual's tolerance for fatigue on the capacity to exert MVT. In 14 young male subjects (10 regular exercisers and 4 sedentary), MVT was measured during isometric knee extension, and voluntary activation (VA), which reflects motor unit activation, was evaluated using the twitch interpolation technique. In addition, the maximum endurance time (ET) was measured, and behavior of the mean power frequency (MPF) and the average rectified value (ARV) of surface EMGs from the vastus lateralis muscle were analyzed during constant force isometric contractions of 60% MVT (short-duration fatigue task; SDF task) and 20% MVT (long-duration fatigue task; LDF task) . Correlations were examined among these five variables.
The results were as follows:
1) Subjects were divided into a high voluntary activation group (HVA group) and a low voluntary activation group (LVA group) . Four sedentary subjects were included in the latter group.
2) MVT was significantly larger in the HVA group than in the LVA group (p<0.01) . A significant positive correlation (r=0.72) was found between MVT and VA (p<0.01) .
3) A significant negative correlation (r=-0.71) was found between MVT and endurance time (ET) for the LDF task (p<0.01) . The ET was significantly longer in the LVA group than in the HVA group (p<0.01) .
4) The MPF of voluntary EMG decreased consistently, as ARV increased during isometric contraction in both tasks (p<0.01), indicating the development of fatigue in the muscle. The final change of MPF relative to the initial value was significantly greater in the SDF task than in the LDF task (p<0.05) .
5) A significant correlation (r=-0.83) was seen between the relative change in MPF and ARV in the SDF task (p<0.01) .
6) For the SDF task, the final change of MPF and ARV relative to the initial value was significantly greater in the LVA group than in the HVA group (p<0.05) .
These results indicate that tolerance for local muscle fatigue usually evaluated as maximum endurance time, may depend on individual differences in VA, the VA, in turn, depending on adapta-tion to exercise, and that there appears to exist a corresponding adaptative strategy of the neuromuscular system during fatiguing contractions. Usefulness of our procedure using the twitch interpolation technique in evaluating muscle fatigue was also suggested.

Myocardial oxidative stress during retrograde continuous blood cardioplegia (RCBC) was evaluated in 35 patients undergoing elective aortocoronary bypass surgery. The patients were divided into three groups: Group C (n=12) received cold (20°C) RCBC, Group T (n=11) received tepid (30°C) RCBC, and Group W (n=12) received warm (36°C) RCBC. Myocardial oxidative stress was assessed by measuring the release of oxidized glutathione (GSSG), malondialdehyde (MDA), and myeloperoxidase (MPO) in the coronary sinus plasma before aortic clamping, at 1, 5, and 10min after unclamping. Myocardial oxygen uptake and lactate release were assessed at the same times. Both the hemodynamic recovery and the creatine kinase MB (CKMB) activity were measured perioperatively until 24h after unclamping. In Group C, a significant coronary sinus release of GSSG was found in the early reperfusion period in comparison to Groups T and W. However, the peak CK-MB activity was significantly lower in Group T than in Group W. No significant difference in the release of MDA or MPO was noted in the three groups. The recovery of oxygen uptake after unclamping was rapid in Group T. The recovery in the left and right ventricular functions and the myocardial lactate release were similar in the three groups. In conclusion, tepid RCBC is considered to protect the myocardium from ischemia-reperfusion injury better than cold or warm blood cardioplegia under retrograde continuous perfusion.

We studied the effects of electro acupuncture stimulation of Baxie on local tolerance as an index of cold induced vasodilation (CIVD). The subjects consisted of 22 healthy adult volunteers. The skin temperature and skin blood flow in the middle finger were simultaneously examined before, during, and after immersion of the finger in cold water. Electroacupuncture stimulation at 1 Hz with a 66 to 100V intensity was performed for 5 minutes. Experiments of no stimulation and electroacupuncture stimulation were conducted on the same subject on different days.
The results revealed the following:
1. CIVD was caused by rapid increase of skin blood flow during immersion of the finger in cold water.
2. The minimum skin temperature, minimum skin blood flow, maximum skin blood flow, mean skin temperature in the rising phase of skin temperature, and the index of resistance to frostbite of the electroacupuncture stimulated group were higher than those of the unstilmulated group, and rates of skin temperature increase and decrease during immersion of the finger in cold water in the electroacupuncture stimulated group were significantly higher. These results show that electroacupuncture stimulation increases local cold tolerance in the finger.

We studied the effects of transcutaneous electrical nerve stimulation (TENS) of Baxie on local cold tolerance as an index of cold induced vasodilatation (CIVD). The subjects consisted of 22 healthy adult volunteers.
The skin temperature and skin blood flow in the middle finger were measured simultaneously before, during, and after immersing the finger in cold water. TENS at 1Hz with 100-V intensity was applied for 5 minutes before immersion of the finger.
Experiments were conducted with no stimulation and with TENS on the same subjects on different days.
The results revealed the following:
1. The minimum skin temperature and minimum skin blood flow during immersion of the finger in cold water were higher in the TENS group than in the control (no stimulation) group.
2. The maximum skin temperature, mean skin temperature, and increase and decrease of skin blood flow rate during immersion of the finger in cold water and in the rising phase of skin temperature were higher in the TENS group than in the control group.
3. The index of resistance to frostbite was higher in the TENS group than in the control group.
4. The number of fluctuations in skin temperature during immersion of the finger in cold water was significantly higher in the TENS group than in the control group.
5. The incidence of abnormal perception during immersion of the finger in cold water was lower in the TENS group than in the control group.
These results suggest that TENS enhances the local cold tolerance of fingers by suppressing SSA in the initial falling phase on skin temperature and increasing the motion of skin blood vessels in the rising phase of skin temperature.

We herein review the early results of minimally invasive coronary artery bypass (MIDCAB). From April 1994 to November 1998, 23 patients underwent MIDCAB, and 12 patients underwent coronary artery bypass grafting with cardiopulmonary bypass (CABG). We compared and analyzed the findings of these two groups. Regarding preoperative factors, the MIDCAB group included elderly patients, while the CABG group consisted of younger patients. However, the frequency of hemodialysis, respiratory disorders and cerebral vascular accidents did not differ significantly between the 2 groups. Regarding perioperative factors, the MIDCAB group needed a shorter operation time, and also had a lower bleeding volume, and a low incidence of blood transfusion. Regarding the postoperative course, the MIDCAB group needed a shorter artificial respiration time, and a shorter postoperative hospital stay, and no mortality was observed. The graft patency of the MIDCAB group was lower (88%) than the CABG group (100%). However, the graft patency of the MIDCAB group reached 94% after we used a stabilizer in the operation. In conclusion, the operation results of the MIDCAB group were comparatively better than those of the CABG group. Thanks to recent technological advances, the results of MIDCAB continue to improve. Though MIDCAB remains an invaluable operative modality for the treatment of one-vessel disease, surgeons must be careful to select appropriate candidates for this operative method.