Objective To investigate the CT feature of small cell lung cancer(SCLC),and investigate the clinical value.Methods 55 patients with SCLC and 118 patients with non -small cell lung cancer(NSCLC)were selected as the study subjects.All the patients were confirmed by pathology,the CT feature of lung cancer were com-pared through unenhanced and enhanced scanning by philips brilliance 16 spiral CT.Results There were significant differences between the SCLC group and NSCLC group in tumor type and morphology(χ2 =22.628,21.249,all P <0.01).Central was the mainly type of SCLC,irregular shape and round was the mainly type of NSCLC.The positive rates of positive airway occlusion,pulmonary atelectasis or obstructive pneumonia in central SCLC group and NSCLC group were 57.78% and 86.27%,62.22% and 98.04%,which in central SCLC group were lower than NSCLC group (χ2 =9.830,20.131,all P <0.01).The Hilar and mediastinal lymph nodes positive rate,fusion of lymph node -pos-itive rate,combined positive rate of violations of mediastinal vessels in central SCLC group and NSCLC group were 90.9% and 49.15%,58% and 15.52%,54.55% and 7.63%,which in central SCLC group were greater than the NSCLC group(χ2 =27.887,53.070,47.294,all P <0.01).Conclusion 16 -slice spiral CT can provide important information for diagnosis of SCLC and NSCLC,the CT signs are conducive to the diagnosis of NSCLC,which contai-ning round mass,bronchial smooth,hilar and mediastinal lymph nodes,lymph nodes are not fused with obstructive pneumonia and atelectasis,mediastinal vascular invasion.

Evidence-Based Medicine ; Humans ; Neck Dissection ; methods

Objective:To investigate the in vitro release behavior and stability of diphenyl diester suspension. Methods:The dis-solution of biphenyl diester dry suspension was detected by HPLC, and the effects of different stirring speed (50, 75, 100 r·min-1 ) and different dissolution media (pH 6. 8 phosphate buffer, 0. 05 mol·L-1 hydrochloric acid solution,water,pH 4. 5 acetate buffer) on dissolution were investigated. The influencing factors testing ( high temperature, high humidity and strong light exposure) , accelerated stability testing[the temperature of (37 ± 5) ℃ and the relative humidity of 76％ ± 5％] and long-term stability testing[(25 ± 3) ℃and the relative humidity of 60％ ± 5 ％] of biphenyl diester dry suspension were carried out. Results:The dissolution behavior of bi-phenyl diester suspension in pH 6. 8 phosphate buffer (50 r·min-1 ) was faster and smoother. The results of influencing factors testing showed that biphenyl diester dry suspension should not be stored under the conditions of high temperature and high humidity. After the samples were stored under the conditions of accelerated testing and long-term stability testing for 6 months, the indicators did not change significantly. Conclusion:The in vitro release of prepared biphenyl diester dry suspension meets the requirements with promis-ing stability.

Academic Dissertations as Topic ; Humans ; Otolaryngology ; trends ; Otorhinolaryngologic Surgical Procedures ; methods ; Thyroidectomy ; methods

Humans ; Laryngeal Neoplasms ; therapy

Arytenoid Cartilage ; surgery ; Contraindications ; Glottis ; Humans ; Laryngeal Neoplasms ; pathology ; surgery ; Laryngectomy

Objective To study the role of membrane cytoskeleton linker Ezrin in the growth and metastasis of human breast carcinoma cell line MCF-7.Methods Small interfering RNA(siRNA)targeting Ezrin were transfected into MCF-7 cells.RT-PCR and Western blot were used to detect the down-regulation of Ezrin mRNA and protein.MTT method was used to detect the proliferation of the cells.Flow cytometry was used to detect the cell cycle.Transwell assay was used to detect the migration ability of the transfected MCF-7 cells.Results RT-PCR and Western blot results revealed that Ezrin siRNA down-regulated Ezrin expression at both mRNA and protein levels.Down regulation of Ezrin expression decreased the proliferation rate of the cell line(t=4.479,P＜0.01).The cell proportion in G2-M phase (t=-5.997,P＜0.01)and the invasion ability(t=5.268,P＜0.01)decreased significantly.Conclusion Ezrin plays an important role in the process of proliferation,migration,and invasion of MCF-7 cells.

Objective To determine the effective prognostic parameter and the best prognostic index of gastiric carcinoma.Methods The prognostic relevance of clinical and pathological variables were evaluated in 83 patients with histologically proved stomach carcinoma (including the expression of nm23, c erbB2, microbloodvessel and PTNM) by the multivariate analysis of COX regression.Result PTNM staging was the only parameter to enter the COX model.Conclusion The PTNM staging is the most important, reliable and best varible factor in predicting the clinical outcome of stomach cancer.

Objective To investigate the changes and significance of serum nitric oxide levels in the early stage after rat liver transplantation.Methods Male Sprague Dawley rats were used as do- nors and recipients of orthotopic liver transplantation,and the time of cold preservation and anhepatic phase was 4 h and 25 min respectively.Forty-eight rats were randomly divided into groups A,B and C (n=16 each).The samples of blood were taken from vena cava and hepatic tissues from left lobe 2 h, 4 h,6 h in groups A,B and C respectively after liver graft reperfusion.ALT and NO levels in serum were detected,and the expression of NF-?B in hepatic tissue was examined by immunohistochemical technique.Amounts of bile flow in 5 min after liver graft reperfused initially were measured.The pathological changes in liver tissues were observed.Results Amounts of bile flow in 5 min in group A (3.73?1.11?l) were greater than those group B (2.35?0.92?l) and group C (2.23?0.81?l) (P