Objective:To study the expression of vascular endothelial growth factor (VEGF) and VEGF mRNA after focal cerebral ischemia reperfusion in rats.Methods:MCAO (Middle Cerebral Artery Occlusion) models were set up in rats,and the rats were divided into six groups:control group,5 groups of ischemia for 2h then respective reperfusion for 6h,12h,24h,48h and 72h.The samples were analyzed with immunohistochemical methods.The expression of VEGF mRNA was detected by RT-PCR analysis.Results:VEGF protein expression started at 6h.It increased remarkably at 24h,and it was strongest at 48h.VEGF mRNA increased at 6h,while reached peak value at 12h and reduced at 24h,finally returned to normal level at 48-72h.VEGF expression was mainly detected in the periphery of infarcts at every timepoint.Conclusion:These findings demonstrate that the VEGF strong expression can be induced in focal cerebral ischemia reperfusion in vivo.The changes of VEGF levels suggest that VEGF has neuroprotective effect against cerebral ischemic injury.

Objective:To evaluate the body mass change in overweight and simple obesity patients 1 year after withdrawal of home-made sibutramine preparation.Methods: Seventy-five overweight or simple obesity patients were enrolled in this Ⅱ phase clinical trial of sibutramine,and they were divided into sibutramine group(n=43) and placebo group (n=32). The patients body mass, body fat rate, waist circumference, serum lipid, blood glucose, blood pressure and adverse effects were determined 12,24 and 48 weeks after withdrawal of sibutramine. Results: There were no significant differences in body mass,body mass index, body fat rate and waist circumference between sibutramine group and placebo group 48 weeks after withdrawal of sibutramine. For sibutramine group,there was no obvious difference before and after treatment, but there was significant difference between immediately and 48 weeks after withdrawal of sibutramine (P

There is a strong support for a role played by adiponectin in the function of ovary and placenta.There is evidence of direct effects of adiponectin on the late stages of folliculogenesis,and additive interactions of adiponectin with insulin and gonadotropins in inducing periovulatory changes in ovarian follicles.Adiponectin has demonstrated a potential effect on ovarian function as well as a possible effect on the formation of placenta,via multiple mechanisms,providing new strategies for the etiology investigation,prevention and treatment of reproductive and related metabolic diseases.

Objective To observe the clinical curative effect of Wumei Pill in the treatment of cold-heat complex diarrhea-predominant irritable bowel syndrome.Methods Modified Wumei Pill was adopted to treat 46 cases of cold-heat complex diarrhea predominant irritable bowel syndrome.Five main symptoms of stool frequency,stool shape and properties,abdominal pain and discomfort,bowel gurgling,vulnerable to the cold were observed.The symptom scores before the treatment and 1 week,2 weeks and 4 weeks after the treatment were evaluated.Results After the treatment of 1 week,2 weeks and 4 weeks,the overall efficiency was 58.70％(27/46),84.78％(39/46),and 97.83％(45/46) respectively,and there were significant differences in curative effect between the different treatment courses (between treatment of 2 weeks and 1 weekx2＝8.571,between treatment of 4 weeks and 2 weekx2=16.426,between treatment of 4 weeks and 1 week x2=35.622).Effective rate of symptoms after 4 weeks treatment were respectively:stool frequency 82.05％ (32/39),stool shape and properties 95.56％ (43/45),abdominal pain and discomfort 92.86％ (39/42),bowel gurgling 85.71％ (18/21),vulnerable to the cold 64.29％ (27/42),and there was obvious differences in effective percentage of five kinds of symptoms between treatment of 4 weeks and 1 week (Stool frequency x2 =7.123、stool shape and properties x2 =13.611、abdominal pain or discomfort x2 =7.721、bowel gurgling x2=10.096、vulnerable to the cold x2=10.769).Conclusion.The clinical effect of Wumei Pill for the treatment of cold-heat complex diarrhea predominant irritable bowel syndrome was defined.

OBJECTIVE:To establish a method for determination of Rifampin in human serum,and to individualize the medication for tuberculosis patients with liver damage.METHODS:Using d-nitrophenol as the internal standard,the serum samples were extracted with methanol.HPLC condition:analytical column:Lichrospher100(4.6?m?250mm),guard column:YWG-C 18 (?4mm?40mm),the mobile phase:methanol-acetonitril-0.04mol/L KH 2 PO 4 (31.5∶31.5∶37,V/V/V),the flow rate:1ml/min,the column temperature:40℃,the wavelength of UV detection:254nm.The serum concentrations were deter?mined at given time periods after patient taking an oral dose of450mg Rifampin,and need of dosage adjustment was judged.RESULTS:The chromatography was good and not interfered by the components of the serum.The dosage of Rifampin had obvious individual difference.After adjusting the dosage,the effect increased and ADRs decreased.CONCLUSION:This is a simple,rapid,accurate,sensitive and reliable method to determine the concentration of Rifampin with a wide concentration range.It is suitable for the analysis of individualized medication of Rifampin.

Polycystic ovary syndrome (PCOS)is the most common cause of anovulatory infertility in women of reproductive age, exerting significant impacts on glucose and lipid metabolism, cardiovascular and endometrial deseases, as well as breast, skin, bone, and psychology fields. As a complex, multi-system of endocrine and metabolic disease, PCOS would be recognized as a component of metabolic syndrome in an overall consideration. The clinical treatment of PCOS is expected to turn simply regulating menstruation and ovulation adjustment into the combination of insulin resistance improvement with antihyperandrogen and ovulation treatment comprehensively.

OBJECTIVE:To evaluate the effectiveness of TCM and antibiotics in the treatment of urinary tract infection(UTI) systematically.METHODS:According to the requirements of Cochrane systematic review,literatures were retrieved from CNKI and Wanfang database.A meta-analysis was performed on 18 literatures conforming to inclusion criteria.RESULTS:Compared with control group,the OR value of treatment group and subgroups including TCM group,TCM combined with antibiotics group,chronic UTI group and senile UTI group were 3.99,3.95,3.95,4.23,4.35,respectively.The 95%CI of those groups were(2.97,5.37),(2.80,5.80),(2.80,5.80),(2.61,6.83),(2.64,7.18).The OR value was of treatment group 0.25(95%CI:0.17,0.37;P

Objective To analyze the epidemiological characteristics of measles in Jilin Province from 2013 to 2020.Methods Through the measles surveillance system of China Disease Prevention and Control Information System,a national health security information project,the measles incidence data of Jilin Province from 2013 to 2022 were collected,the measles incidence and incidence characteristics were analyzed by descriptive epidemiological method,while the virus was isolated and the genotype was identified at the same time.Results From 2013 to 2022,3 202 measles cases were reported in Jilin Province,and the reported incidence rates were 0. 57/100 000,9. 88/100 000,0. 71/100 000,0. 32/100 000,0. 12/100 000,0. 13/100 000,0. 13/100 000,0. 05/100 000,0. 05/100 000 and 0. 06/100 000,respectively.By age group,people aged 0 ～ 1,2 ～ 6,7 ～ 18,19 ～ 29,30 ～ 49 and over 50 years accounted for 42. 19%,10. 31%,6. 28%,14. 52%,24. 83% and 1. 87% of all the reported incidence cases,respectively,while among cases aged less than8 months,8 ～ 12 months,2 ～ 18 years and over 19 years,98. 49%,71. 34%,33. 33% and 39. 77% had no vaccination history,respectively. From 2013 to 2018,a total of 44 measles virus strains were obtained in the measles laboratory of Jilin Province,except for one vaccine strain A genotype,the rest were all H1a genotype,and no measles virus strain was obtained since 2019.Conclusion From 2013 to 2022,the reported incidence of measles in Jilin Province showed a downward trend year by year,while there is still a need to maintain the coverage of two doses of vaccination of measles containing vaccine. At the same time,adult measles vaccination should be strengthened,and the sensitivity of surveillance system should be improved to prevent measles outbreaks.

Clostridiodes difficile(C.difficile)is the most common causative agent of antibiotic-associated diarrhea(ADD)in the world. In recent years,with the emergence of highly resistant and virulent strains,the outbreaks of C.difficile infection have occurred around the world. The incidence,recurrence and mortality of C.difficile infection are on the rise worldwide,and bring great challenges to clinical treatment. Pathogenic strains mainly produce two homologous glycosylation toxins A and B,which can cause symptoms ranging from diarrhea to highly lethal toxic megacolon. In view of the malignant consequences caused by C.difficile infection,disease prevention is still an important way worth exploring. Until now there is no approved vaccine against C.difficile. Therefore,this review assessed the status and challenge of clinical trials of vaccine research for C.difficile.

Objective To develop a method for monoclonal screening of CHO engineered cell lines using Verified In-situ Plate Seeding(VIPS). Methods Cell pool 1 stably transfected with target gene was inoculated by using monoclonal inoculation/imaging equipment(VIPS)in 96-well plates containing culture medium of different combinations(18 combinations,numbered 1～18)and volumes(100 and 200 μL/well). Monoclonal origin tracing pictures were taken,according to which,the monoclonal inoculation rate,clone formation rate and monoclonal proliferation rate were calculated and the image effects of monoclonal origin were evaluated. The applicability of this method was verified by cell pool 2,cell pool 3 and cell pool 4 stably transfected with target gene. Results The optimum monoclonal medium was Medium Ⅱ,and the volume of medium was 100 μL/well. Under the optimum process conditions,the monoclonal inoculation rate of Cell pool 1 was 80%,the clone formation rate was 83%,the proliferation rate was fast,and the monoclonal origin image was clear. While for Cell pool2,Cell pool 3 and Cell pool 4,the average rate of monoclonal inoculation was 78%,the average rate of clone formation was67%,the proliferation rate was slightly different and the image of cell division process was clear under the optimum process conditions. Conclusion In this study,the monoclonal screening method of CHO engineered cell lines developed using VIPS can improve the clone formation rate of CHO engineered cell lines and provide sufficient proof of monoclonal origin.