Objective:To evaluate the body mass change in overweight and simple obesity patients 1 year after withdrawal of home-made sibutramine preparation.Methods: Seventy-five overweight or simple obesity patients were enrolled in this Ⅱ phase clinical trial of sibutramine,and they were divided into sibutramine group(n=43) and placebo group (n=32). The patients body mass, body fat rate, waist circumference, serum lipid, blood glucose, blood pressure and adverse effects were determined 12,24 and 48 weeks after withdrawal of sibutramine. Results: There were no significant differences in body mass,body mass index, body fat rate and waist circumference between sibutramine group and placebo group 48 weeks after withdrawal of sibutramine. For sibutramine group,there was no obvious difference before and after treatment, but there was significant difference between immediately and 48 weeks after withdrawal of sibutramine (P

Objective:To study the expression of vascular endothelial growth factor (VEGF) and VEGF mRNA after focal cerebral ischemia reperfusion in rats.Methods:MCAO (Middle Cerebral Artery Occlusion) models were set up in rats,and the rats were divided into six groups:control group,5 groups of ischemia for 2h then respective reperfusion for 6h,12h,24h,48h and 72h.The samples were analyzed with immunohistochemical methods.The expression of VEGF mRNA was detected by RT-PCR analysis.Results:VEGF protein expression started at 6h.It increased remarkably at 24h,and it was strongest at 48h.VEGF mRNA increased at 6h,while reached peak value at 12h and reduced at 24h,finally returned to normal level at 48-72h.VEGF expression was mainly detected in the periphery of infarcts at every timepoint.Conclusion:These findings demonstrate that the VEGF strong expression can be induced in focal cerebral ischemia reperfusion in vivo.The changes of VEGF levels suggest that VEGF has neuroprotective effect against cerebral ischemic injury.

OBJECTIVE:To establish a method for determination of Rifampin in human serum,and to individualize the medication for tuberculosis patients with liver damage.METHODS:Using d-nitrophenol as the internal standard,the serum samples were extracted with methanol.HPLC condition:analytical column:Lichrospher100(4.6?m?250mm),guard column:YWG-C 18 (?4mm?40mm),the mobile phase:methanol-acetonitril-0.04mol/L KH 2 PO 4 (31.5∶31.5∶37,V/V/V),the flow rate:1ml/min,the column temperature:40℃,the wavelength of UV detection:254nm.The serum concentrations were deter?mined at given time periods after patient taking an oral dose of450mg Rifampin,and need of dosage adjustment was judged.RESULTS:The chromatography was good and not interfered by the components of the serum.The dosage of Rifampin had obvious individual difference.After adjusting the dosage,the effect increased and ADRs decreased.CONCLUSION:This is a simple,rapid,accurate,sensitive and reliable method to determine the concentration of Rifampin with a wide concentration range.It is suitable for the analysis of individualized medication of Rifampin.

OBJECTIVE:To evaluate the effectiveness of TCM and antibiotics in the treatment of urinary tract infection(UTI) systematically.METHODS:According to the requirements of Cochrane systematic review,literatures were retrieved from CNKI and Wanfang database.A meta-analysis was performed on 18 literatures conforming to inclusion criteria.RESULTS:Compared with control group,the OR value of treatment group and subgroups including TCM group,TCM combined with antibiotics group,chronic UTI group and senile UTI group were 3.99,3.95,3.95,4.23,4.35,respectively.The 95%CI of those groups were(2.97,5.37),(2.80,5.80),(2.80,5.80),(2.61,6.83),(2.64,7.18).The OR value was of treatment group 0.25(95%CI:0.17,0.37;P

Objective To observe the clinical curative effect of Wumei Pill in the treatment of cold-heat complex diarrhea-predominant irritable bowel syndrome.Methods Modified Wumei Pill was adopted to treat 46 cases of cold-heat complex diarrhea predominant irritable bowel syndrome.Five main symptoms of stool frequency,stool shape and properties,abdominal pain and discomfort,bowel gurgling,vulnerable to the cold were observed.The symptom scores before the treatment and 1 week,2 weeks and 4 weeks after the treatment were evaluated.Results After the treatment of 1 week,2 weeks and 4 weeks,the overall efficiency was 58.70％(27/46),84.78％(39/46),and 97.83％(45/46) respectively,and there were significant differences in curative effect between the different treatment courses (between treatment of 2 weeks and 1 weekx2＝8.571,between treatment of 4 weeks and 2 weekx2=16.426,between treatment of 4 weeks and 1 week x2=35.622).Effective rate of symptoms after 4 weeks treatment were respectively:stool frequency 82.05％ (32/39),stool shape and properties 95.56％ (43/45),abdominal pain and discomfort 92.86％ (39/42),bowel gurgling 85.71％ (18/21),vulnerable to the cold 64.29％ (27/42),and there was obvious differences in effective percentage of five kinds of symptoms between treatment of 4 weeks and 1 week (Stool frequency x2 =7.123、stool shape and properties x2 =13.611、abdominal pain or discomfort x2 =7.721、bowel gurgling x2=10.096、vulnerable to the cold x2=10.769).Conclusion.The clinical effect of Wumei Pill for the treatment of cold-heat complex diarrhea predominant irritable bowel syndrome was defined.

There is a strong support for a role played by adiponectin in the function of ovary and placenta.There is evidence of direct effects of adiponectin on the late stages of folliculogenesis,and additive interactions of adiponectin with insulin and gonadotropins in inducing periovulatory changes in ovarian follicles.Adiponectin has demonstrated a potential effect on ovarian function as well as a possible effect on the formation of placenta,via multiple mechanisms,providing new strategies for the etiology investigation,prevention and treatment of reproductive and related metabolic diseases.

Polycystic ovary syndrome (PCOS)is the most common cause of anovulatory infertility in women of reproductive age, exerting significant impacts on glucose and lipid metabolism, cardiovascular and endometrial deseases, as well as breast, skin, bone, and psychology fields. As a complex, multi-system of endocrine and metabolic disease, PCOS would be recognized as a component of metabolic syndrome in an overall consideration. The clinical treatment of PCOS is expected to turn simply regulating menstruation and ovulation adjustment into the combination of insulin resistance improvement with antihyperandrogen and ovulation treatment comprehensively.

The results of the rat hepatocellular carcinoma (HCC) induced by purified afla-toxin B1 (AFB1)were as follows. (1) The precancerous lesions included hepatocytic dysplasia (58.6%), eosinocytic proliferative foci (51.61%) with most positive results by indirect immunoperoxidase-labelling technique for ?-fetoprotein (IIP-AFP), and basophilic proliferative foci with all negative results by IIP-AFP. (2) In 11 cases of HCC, there were 7 with multicentric origin and 4 with single origin, and 9 of 11 cases were in gradeⅠ differentiation and 2 in grade Ⅱ differentiation. (3) In 11 cases of HCC, 7 were of large cell type of HCC and 4 small cell type of HCC. (4) The specimens of 6 cases of HCC were examined by electronic microscopy, and it was found that the differentiation in the large cell type of HCC was better than that in the small cell type of HCC. The authors assume that there may exist multicentric and single origins in the histogenesis of HCC, and AFB1 is likely to be a carcinogen of human non-cirrhotic HCC.

Thirty one male Wistar rats weighing 130-150 g were continuously fed with aflatoxin B1 (AFB1) for 45 weeks.Each rat received 3.89mg of AFB1 crystals in total close.Three treated rats and one control ret were killed at the 8th, 9th, 11th, 12th, 13th, 14th and 1.3th month, respectively, and seven rats died naturaly after the 17th month.Histological sections of the liver were stained with hematoxylin-eosin.Morphological changes of 28 rats' liver tissues stained with enzyme-labelled immuno-fetoprotein, and ultrastructural chinges of the liver specimens from 15 rats were observed .Histologicall ', the main manifestations of liver cells included hydratoid degeneration, eosinophilic degeneration, basophilic change.adipose degeneration and focal necrosis.The answer reaction showed oval cell and bilbary proliferation, hepatocellular regeneration and inflammatory cell infiltration.All of the above changes were chronic non-specific toxic hepatitis. The ultrastructural changes showed nucleolar uniformation, nucleoid body formation, mitochondria swelling and matrix uniformation, and vesicle formation and focal necrosis. 85.71 % 24/28)of the alpha-fetoprotein positive hepatocytes were found in host liver tissues.The AFP positive material mostly distributed in all of the plasma of liver cells.AFP positive hepatocytes were dispersed or focal in the liver tissue. This result suggests that the liver cell atavism mav be the toxic effect of aflatoxin B1.

Observations on 142 cases of noncirrhotic hepatocellular carcinoma (NCH) (135 surgical operating specimens, 8 autopic cases) were made. 50 cases of cirrhotic hepatocellular carcinoma (CH) (42 surgical operating specimens, 8 autopic cases) were taken as the control. The results were as follows:(1)In both groups, the hepatocellular carcinoma (HCC) had a similar background of liver diseases. 51.41% of the cases of hepatitis B and 48.59% of the cases of"orcein negative hepatitis" were associated with NCH,while 58.00% of the former and 42.00% of the latter were associated with CH. In both groups in the cases of HCC were included 5 cases of primary multiple carcinoma. We considered that pathogenesis of HCC were synthetic factors. It was assumed that NCH is due to weak carcinogen, hepatic parenchyma is lightly destroyed, and as the human body has hypersusceptibility to HCC, the precancerous stage is short and cancer occurs early.(2)In 80.99% of the NCH cases and 100% of the CH cases was found proliferation of oval cells. The difference between the two groups of HCC was statistically significant.The proliferation may be a repairing reaction. Owing to the action of car-cinosen, the poorly differentiated HCC may be derived from the disturbances of oval cells.(3)In 29.57% of the cases NCH and 70.00% of the cases CH was found hepar tocyte dysplasia, and the difference between the two groups of HCC statistically significant. It was indicated that dysplasia may be precancerous disoders.Oval cell proliferation and hepatocyte dysplasia of the NCH group are less than those of the CH group.It is suggested that liver tissues of the NCH group are slightly damaged, hence the incidence of NCH is lower than that of CH.