Adult ; Anaphylaxis ; etiology ; Female ; Humans ; Male ; Semen ; immunology

Objective To explore the animal model making method for diarrhea-predominant irritable bowel syndrome (IBS-D) caused by the disharmony of liver and spleen in TCM.Methods In order to define the period of the animal model making,the 32 neonatal rats were divided into 3 days group,6 days group,9 days group and control group.In order to determine the weight of the experimental rats,the 48 rats with different weight were divided into lower weight model group,lower weight control group,medium weight model group,medium weight control group,higher weight model group and higher weight control group.In order to decide the method of causing diarrhea,the 32 rats were divided into lactose enriched diet group,restraint stress group,lactose enriched diet plus restraint stress group,and a control group.The body weight and diarrhea rate of all groups were compared respectively.After the method of model making was decided,the 16 rats were divided into a model group and a control group,and 5-hydroxytryptamin (5-HT),lactate dehydrogenase (LDH) and aquaporin 4 (AQP4) of the two groups were compared for model-proving.Results Based on the results step by step,the best method was decided:the period of the animal model making was 6 days,rats with higher body weight should be subjects,and the lactose enriched diet combining with restraint stress should be used to induce diarrhea.Model-proving test:compared with the control group,5-HT and LDH decreased (P

Objective To screen and identify protein interacted with enterovirus 71 (EV71)3A protein by means of T7-phage display system.Methods The prokaryotic expression vector of 3A protein was constructed for expressing and purifying 3A pro-tein.With 3A protein as the target protein,the T7-phage display system was adopted to screen the human hepatocyte cDNA library. The screened products were performed the DNA sequence analysis and the homologous research.Results 3A protein was expressed and purified,after 4 rounds of biopanning,37 positive clones were selected.The inserted fragments were amplified by PCR using specific T7 primer.The products were sequenced,2 proteins were identified to interact with 3A protein by homologous analysis. Conclusion Trough the T7 phage display system,two proteins interacted with 3A are obtained.The possible function of 3A protein can be speculated by researching the function of these two proteins,which lays the foundation for the further studying the pathogen-ic mechanism of EV71.

Objective: This study was undertaken to access the impact of hyperthermic chemotherapy (HTCT) on human gastric cancer cells in vitro. Methods: Six human gastric cancer cell lines (AGS, MKN45, SGC7901, NCI-N87, SNU-1 and SNU-16) and gastric cancer cells originated from clinical patients were used in this study. Treatment conditions were categorized into 4 modes: normothermic control (NT, 37°C), hyperthermia (HT, 43°C), normothermic chemotherapy (NTCT) and HTCT. The cells were treated with different concentrations of cis-diammine dichloroplatinum (CDDP, 0 to 32 mg/L) in combination with hyperthermia for 2 h. MTT assay was adopted to evaluate cell proliferation and cytotoxicity after HTCT. Preliminary morphological changes were observed under light microscope (LM) while ultrastructure changes and the definite cell death type were determined by transmission electron microscopy (TEM) and fluorescent microscopy (FM). The quantitative ratio of apoptosis and necrosis was determined by Annexin V-FITC and PI double staining using flow cytometry (FCM). Results: According to MTT assay and LM observation, there was a synergistic effect between hyperthermia and chemotherapy in inhibiting proliferation of the different types of gastric cancer cells. Hyperthermia enhanced the sensitivity of gastric cancer cells to CDDP chemotherapy. The concentration of CDDP required for inhibiting cell proliferation and inducing cell death was much lower in HTCT group than that of NTCT group. TEM and FCM examination showed that HTCT induced both apoptosis and necrosis of AGS and SNU-1 cells and apoptosis was the major type of cell death. Conclusion: There exists significant synergistic effect between hyperthermia and chemotherapy in inhibiting the proliferation of gastric cancer cells. Hyperthermia enhances the sensitivity of gastric cancer cells to chemotherapy. HTCT induces two patterns of cell death, apoptosis and necrosis, in gastric cancer cells. Apoptosis was the major type of cell death.

Child ; Cough ; chemically induced ; Disinfectants ; adverse effects ; Humans ; Irritants ; adverse effects ; Male

One of the major responsibilities for medical students is to save lives and to popularize emergency medical treatment knowledge for the masses of society.Based on research analysis,systemic training of cardiopulmonary resuscitation program including lectures,simulation education and social practice were performed for medical students.After practice activities,these students could proficiently apply the basic knowledge and skills of cardiopulmonary resuscitation in practical work and spread what they have learned to the public.Furthermore the program could improve students' abilities in social practice and team spirit and it is of great social significance.

Objective To study the mutations of Pseudomonas aeruginosa oprD gene in Imipenem-resistant clinical isolates. Methods Random amplified polymorphic DNA typing(RAPD) was carried out to analyse the homology in 10 Imipenem-resistant clinical isolates. Pseudomonas aeruginosa oprD gene in 10 Imipenem-resistant clinical isolates were amplified by polymerase chain reaction and sequenced. Results Ten Imipenem-resistant clinical isolates were divided into four different clones which can not produce metallo ?-lactamase. As compared with sequence X63152, oprD gene of Pseudomonas aeruginosa varied greatly. The aberration rates exceed 50%. There were multiple point mutations within 276～387 bp coding region of 30, 11, 9, 20, 31 strains. Due to the mutations of 308 bp G→C and 344 bp C→A, threonine and diaminocaproic acid were replaced by serine and threonine respectively. The DNA deletion of 393～412 bp in oprD gene contributes to the frame shift mutation in the following nucleotide sequence. The deletion of 264～273 bp in the coding region of oprD gene in 13 and 21 clone strains leads to frame shift mutations forming terminal codon TAA(319～321 bp).Base substitutions and multiple point mutations were obvious in the coding regions of 1 and 22 clone strains. Their aberration rates were 54.03% and 74.89% respectively. Conclusions The mutations of Pseudomonas aeruginosa oprD gene in Imipenem-resistant clinical isolates are various.

AIM To study comparision of water-soluble components in Danshen from different sources. METHODS To be determined by HPLC, the eluent consisted of methanol (1 % acetic acid)-water (1 % acetic acid). A gradient method was set up. The detection wavelength was set at 280 nm. RESULTS The average recovery of four components were all above 97 % , RSD was no more than 2.0 % . The calibration curve was linear. CONCLUSION The method is accurate and reliable with good reproducibility.

Objective: To study the expressions of vascular endothelial growth factor D (VEGF-D) and its receptor fms-like tyrosine kinase-4 (Flt-4) and to determine whether the presence of VEGF-D and Flt-4 was associated with the clinicopathologic characteristics and prognosis of pancreatic cancer. Methods: The expressions of VEGF-D and Flt-4 proteins were examined by immunohistochemical staining in 48 pancreatic cancer tissues, 32 adjacent pancreatic tissues, and 13 normal pancreatic tissue samples. Differences in distribution of VEGF-D or Flt4 were analyzed using the χ2 test. Kaplan-Meier survival analysis was used to estimate survival time and log-rank test was used to compare differences in survival time between the patients with or without VEGF-D or Flt-4 expression. Results: Immunohistochemical analysis revealed that the positive staining rates of VEGF-D and Flt-4 in pancreatic cancer tissues were significantly lower than that in para-cancerous tissues (P < 0.05), but significantly higher than that in normal pancreatic tissues (P < 0.05). Pancreatic cancer tissues with lymph node metastasis had higher expression of VEGF-D and Flt-4 compared with those without lymph node metastasis (P < 0.01). The median survival time and 1-, 2-, 3-year survival rate of VEGF-D-or Flt-4-positive patients were significantly decreased compared with VEGF-D- or Flt-4-negative patients (P < 0.05). Conclusion: VEGF-D and Flt-4 may contribute to lymph node metastasis and may serve as a prognostic factor for pancreatic cancer patients.

AIM: To study the pharmacological effect of Weichangshu (WCS) (Rhizoma coptidis, Fructus Evodiae., etc.) on rodent gastrointestinal function and gastric mucosa, and to analyze its analgesic and antidiarrheal mechanism. METHOD: Mouse's hypermobility induced by neostigmine and its gastric emptying were treated by WCS in comparison with normal subjects, rat's gastric ulcer induced by absolute ethanol, diarrhea induced by castor oil, pain induced by acetic acid and rat's gastric secretion and absorption were observed. RESULTS: The experimental results showed that WCS did not affect normal mouse's intestinal propulsion but significantly antagonized neostigmine induced intestinal propulsion and inhibited the gastric mucosa injure and decreased the frequency of the writhe and limited diarrhea. CONCLUSION: WCS has power to recover gastrointestinal function and lessen gastric mucosa injury and has analgesic and antidiarrheal effect.