Endothelial monocyte-activating polypeptide-Ⅱ (EMAP-Ⅱ ) is a novel proinflammatory cytokine with proinflammatory,proapoptotic and antiangiogenic properties.It is associated with many tumorassociated proteins,such as tumor necrosis factor,vascular endothelial growth factor,hypoxia inducible factor-1α,arginyl-tRNA synthetase,and insulin-like growth factor- Ⅰ.EMAP-Ⅱ has anti-tumor properties and has a good prospect in cancer prevention and treatment.

Objective:To investigate the different function of dendritic cells between patients with lupus nephritis and healthy control,and discuss the pathogenesis of the disease.Methods: Choose 50 healthy controls,50 patients with lupus nephritis,and isolation of blood dendritic cells.Then samples were induced with GM-CSF and IL-4,and cells were collected on day 7.DC maturity was evaluated using the following methods:fluorescence-activated cell sorting analysis for cell surface markers;enzyme-linked immunosorbent assay for cytokine production;cell proliferation assay for induction of T cell proliferation.Results: Compared with the control group,lupus nephritis group had increased MHC Ⅱ,CD86,CD80,and CD40 expression(P<0.05);displayed the increased IL-12 and TNF-αlevels(P<0.05);displayed a increased ability to drive lymphocyte cells proliferation (P<0.05).Conclusion:Compared with the control group ,dendritic cells in lupus nephritis group displayed the mature condition ,which indicates that dendritic cells play the important role in the pathogenesis of lupus nephritis.

Objective To investigate the association between serum non-organ-specific autoantibodies and polycystic ovary syn-drome(PCOS).Methods A total of 69 patients with PCOS in child-bearing period treated in our hospital were selected as the study group and contemporaneous 69 healthy child-bearing year women were selected as the control group.Serum level of antinuclear anti-bodies (ANA)was measured by ELISA,serum level of anti-dsDNA was measured by colloidal gold spot infiltration assay and the extractable nuclear antigen (ENA)auto-antibodies profiles were measured by Western blot.Results Serum levels of ANA and an-ti-dsDNA antibodies in the study group were significantly elevated compared with the control group with statistical difference.Con-clusion Serum autoantibody positive exists in the patients with PCOS.

Objective To evaluate the effectiveness of periodontal mechanical therapy on periodontal health and interleukin-6 (IL-6) levels in gingival crevicular fluid (GCF) in type 2 diabetic patients with chronic periodontitis. Methods GCF samples were obtained with filter papers in first molar sites from 37 type 2 diabetic patients with chronic periodontitis. The randomized controlled and blinded clinical trial with a duration of six months was designed to compare effects between three groups of professional mechanical tooth cleaning (PMTC), i.e. coronal scaling (group Ⅱ) with periodontal initial therapy (group Ⅰ) and without clinical therapy (control group). The clinical periodontal index such as probing depth (PD), attachment loss (AL), modified bleeding index (mBI) and bacteria plaque index (PLI) were obtained with a Williams type periodontal probe. Laboratory examination including GCF volume, concentrations and total amounts of IL-6 in GCF were detected with ELISA method. Results Significantly decreasing trends of PD, AL, mBI, GCF volume and total amounts of IL-6 were observed in group Ⅰin all test period. The group Ⅱ had a significant reduction of PD at the third month as compared with baseline (⊿=0.36 mm, P＜0.05), and the other figures showed descending trends but didn′t present statistical significances. At the end of study, the group Ⅰand Ⅱ had significant reductions of GCF volume compared with increases of control group (0.96 μ1＞0.03 μ1mm＞-0.20 μ1, P＜0.05). Conclusions The sequential periodontal supra- and sub- gingival scaling has definitive effects on periodontal health improvement and on reducing the IL-6 level in GCF in type 2 diabetic patients.

0.05). The data proved that PSMA7 was overexpressed in pcDNA3.1(-)/PSMA7-transfected A549 cell line, both in mRNA level and in protein level. Conclusion Eukaryotic expression plasmid pcDNA3.1(-)/PSMA7 has been successfully constructed, and the PSMA7 is stably expressed in A549 cell line. This would pave the way for further study of PSMA7.

Combination chemotherapy and nanoparticle drug delivery are two promising strategies in cancer treatment. The use of multiple therapeutic agents in combination provides synergistic effects among different drugs against cancer cells and suppresses drug resistance through distinct mechanisms of action.Nanocarriers can improve anti-tumor effects of drugs and reduce systemic toxicity through delivering drugs into the tumor tissue specially. Recently, many studies are aiming to encapsulate multiple agents into nanocarriers to optimize the anti-tumor effects. In the present review, the recent advances of nanoparticle platforms applied with co-delivering two or more drugs were summarized and the various combination strategies based on nanoparticles in oncology were discussed.

BACKGROUND:Intracerebral administration of selective drugsviathe carotid artery is currently one of the effective methods to enhance the drug concentration in the brain and reduce the influence of drugs on other system functions. OBJECTIVE:To establish the muscle-relaxation rabbit models by infusing propofol continuously in the internal carotid artery and analyze the variations of propofol concentration. METHODS: The muscle-relaxation rabbit models were established by continuously infusing propofol at a constant speedviacatheterization in the internal carotid artery. The pharmacokinetic characteristics could be analyzed by the methods of obtaining arterial and venous blood on both sides of neck and samples of brain tissue on both sides in different points, detecting drug concentration using high pressure liquid assay, and then mathematicaly conversing the resulting data for fitting processing and statistical regression. RESULTS AND CONCLUSION:The method of determining the concentration of propofol using high pressure liquid assay is feasible, stable and reliable. Through investigating the concentration of propofol infused via the carotid artery at different time points, we discovered that the growth rate distribution of propofol concentration and data distribution are in log-normal distribution profile which belong to non-exponential kinetics model,i.e., modified log-normal distribution model,??)(ln x μ 2 1 fx ()=e 2σ2 , whereσ is the range of drug concentration growth indicating stability xk 2πσ of concentration changes, which is an integrated variable related to various factors, such as brain tissue uptake of drugs and brain circulation. The pharmacokinetic model of continuously infusing propofol in the internal carotid artery belongs to log-normal distribution function, i.e., a non-exponential function kinetics model. The brain concentration variations on both sides changing over time folow log-normal distribution function law.

Objective:To investigate the effect of HSPC 238 overexpression or low expression on the regulation of the target protein ( HMOX1, MT2A, UBB, RPS27a ) and the regulation pathways.Methods: To construct the interference vector and overexpression vector of HSPC238 respectively,transfected the HEPG2 cell lines by the liposome method,detect the expression of mRNA and protein of the HSPC 238 and the target proteins by the RT-PCR and Western blot , further to transfer the overexpression plasmids of the target proteins into the HEPG 2 cell lines which had been transfected with interference vector and overexpression vector of HSPC238,the experimental group cell lines were treated with proteasome inhibitor MG 132,to purify the target proteins with nickel column,do immunoblotting with HSPC238 to detect the accumulation situation of the target proteins.Results: The HMOX1,MT2A, RPS27a were downregulated obviously when the HSPC 238 was interfered exogenous;and the MT2A,UBB,RPS27a were up-regulated after the HSPC238 overexpressed.The overexpression plasmid of target proteins were transfected into the HEPG 2 cell lines which have been transfected with interference vector and overexpression vector of HSPC 238 ,compared with the control group ,the target protein band in the experimental group was significantly increased after treatment with the proteasome inhibitor MG 132.Conclusion:The HSPC238 overexpression can upregulate the expression of MT 2A,UBB and RPS27a,and interfering HSPC238 can downregulate the expression of HMOX1,MT2A and RPS27a;the HSPC238 target protein may play a regulatory role through the UPP pathway;the HSPC238 may play a regulatory role on the target proteins through the UPP pathway.

Objective To explore the correlation between self-concept and subjective quality of life among children with tic disorders (TD). Methods The Piers-Harris Children's Self-concept Scale (PHCSS) and the Inventory of Subjective Life Quality (ISLQ) were complet-ed by 65 children with TD and 71 normal children from March to September, 2015; and analyzed with single factor analysis, correlation analysis and multi factors regression analysis. Results There was no significant difference in PHCSS scores between the TD children with and normal ones (t<1.624, P>0.05). The ISLQ scores of school life, self-awareness and cognitive component were less in the TD children than in the normal ones (t>2.051, P<0.05). The PHCSS subscores correlated with ISLQ subscores (P<0.05), except the following:PHCSS scores of behavior did not correlate with ISLQ scores of peer relationship, school life, living environment, self-awareness and cognitive com-ponent;PHCSS scores of intelligence and school environment did not correlate with ISLQ scores of family life, peer interaction and the liv-ing environment;PHCSS scores of physical appearance and properties did not correlate with ISLQ scores of family life and living environ-ment;PHCSS scores of anxiety did not correlate with ISLQ scores of family life, peer interaction and cognitive component;PHCSS scores of affiliation did not correlate with ISLQ scores of peer interaction;and PHCSS total score did not correlate with ISLQ scores of family life and living environment. PHCSS scores of affiliation were the factor related with the quality of life (β=0.301, P<0.05). Conclusion The quali-ty of life may be impaired in the children with TD, which may associate with the self-concept and need further intervention.

Objective:To construct a bait vector for HSPC238, and to screen the target proteins which interact with the HSPC238.Methods:Gene synthesis method was used to synthetic gene HSPC238, then connected with the pGBKT7 vector after digesting by the sfiIA and sfiIB,to obtain the bait plasmid pGBKT7-HSPC238,then transferred into the yeast strains AH109 with the empty plasmid pGBKT7after sequencing,to observe its self-activating effect in the nutrient deficiencies medium,and further to screen the target proteins which interact with HSPC238 from the human fetal liver cDNA library.Results:The bait vector pGBKT7-HSPC238 was successfully constructed,and it had no self-activating effect through the phenotypic screening,after the yeast two-hybrid technology with literature analysis,we preliminary screened and found that the ribosomal protein L5(RPL5) may be the one of the target proteins which interacted with HSPC238 from the human fetal liver cDNA library.Conclusion: We successfully constructed the bait plasmid vector PGBKT7-HSPC238,and after the yeast two-hybrid technology with literature analysis,we preliminary screened and found that the ribosomal protein L5( RPL5) may be the one of the target proteins which interacted with HSPC238.