OBJECTIVE To inspect the condition of hands-washing among medical staff in order to improve it accordingly.METHODS Direct inspection on the effect of hands-washing among medical staff from all departments within hospital at a fixed interval and make statistics as well as analysis retrospectively.RESULTS The qualified rate of hands-washing respectively was 90.03%,91.47%,91.54%,and 93.55% during the years of 2003 through 2006.The rate had risen steadily.The qualified rate of hands-washing in key departmeuts and in others was 89.15% and 87.87%.respectively,without significatnt differences.However,the qualified rate among nurses and doctors was 93.80% and 87.17%,respectively.The qualified rate among nurses was obviousely higher than among doctors in this regard with significant differences between them.CONCLUSIONS Intensifying training and standardizing hands-washing methods by means of choosing suitable hands-detergents are important in order to improve the compliance of hands-washing among medical staff.

OBJECTIVE To explore the distribution,bacterial types and antibiotic-resistance in a children hospital.METHODS The methods of combining objective investigation with the(doctor′s) report were adopted.RESULTS In our hospital,the rate of upper airway infections was in the first accounted for 42.4%,mainly ocourred in internal neurology,hematology and nephrology departments and gastrointestinal and cardiosurgery departments.(Among) Gram-negative robs,Pseudomonas aeruginosa was the principle of the upper airway infections,it was(sensitive) to ceftazidime.Among Gram-positive cocci,the Staphylococcus aureus and Enteroccoccus were the main pathogens concerned, which were(sensitive) to vancomycin and nitrofurantoin.CONCLUSIONS Long-hospitalization,unreasonable use of hormone and(antibiotic).invasive operation and various diseases in the same department are the main risk factors,for the correspondent(measures) must apply to prevent and control the hospital infection.

Objective Using the Coomassie briliant blue method to solve the problem of the precipitation when detecting the protein content in chitosan.Methods Improving the Coomassie briliant blue method.Results Use the Coomassie briliant blue solution with hydrochloric acid concentration of 5% as stain, stain 15 min , the maximum absorption wavelength is 598 nm. the standard curve of absorbance-protein concentration isy=0.003x+0.017 9.r2=0.991, the linear correlation is good within the protein concentration scope of 5μg/mL~40μg/mL, the lowest detection limit is 1μg/mL, and its recovery is 97.8%~103.7%.Conclusions In this paper, the method of determining the content of protein in chitosan is high accurate, stable, reliable and reproducible, and can be widely used in product testing.