1.The characterization of serum hepatitis B virus full-length genome in chronic severe hepatitis B patients and its clinical significance
Suyuan HUANG ; Demin YU ; Feng LIU ; Li CHEN ; Yue HAN ; Xinhua LI ; Xinxin ZHANG
Chinese Journal of Infectious Diseases 2011;29(7):401-405
Objective To characterize serum hepatitis B virus(HBV)full-length genome quasispecies and to investigate its ralationship with severe exacerbation of chronic hepatitis B(CHB).Methods HBV full-length genome was amplified and cloned from four treatment naive CHB patients and four treatment naive CSHB patients.Fourteen to sixteen clones per sample were selected,sequenced and analyzed by bioinformatics software.The measurement data was compared by independent-samples t test and count data was analyzed by x2 test. Results Totally 120 HBV fulllength genome sequences were obtained.All the patients had either genotype B or C virus monoinfection.One hundred percent clones(60/60)from CSHB patients showed mutations including G1896A,A1762T/G1764A(one patient even carried A1762T/G1764A/C1766T mutations),T1753C/G and start codon mutations in preS2,preS1,which were more common than those from CHB patients(46/60,76.7%;x2=15.85,P<0.01).The quasispecies complexity and diversity were higher in CSHB patients than CHB patients within full-length genome,S,X,P genes and reverse transcriptase region,but lower within C gene at both nucleotide and amino acid levels.But the difference were not statistically significant in all regions.Conclusion The mutation frequency and quasispeeies heterogeneity in HBV genome are higher in CSHB patients than in CHB patients,which may play a role in the severe exacerbation of CHB and needs further investigation in large scale studies.
2.Preliminary study on association of hepatitis B virus S gene quasispecies with clinical outcome of HBV infection
Jialun YU ; Demin YU ; Jiehong JIANG ; Donghua ZHANG ; Suyuan HUANG ; Feng LIU ; Xinxin ZHANG
Chinese Journal of Clinical Infectious Diseases 2011;04(1):16-20
Objective To investigate the association of hepatitis B virus(HBV) S gene quasispecies with the outcome of HBV infection.Methods Serum samples were collected from three chronic HBV carriers, three chronic hepatitis B and three chronic severe hepatitis B patients.All subjects were male and with HBV genotype C.HBV S gene was amplified, and 20 clones of HBV fragment were randomly selected and sequenced from each sample.SPSS 15.0 software was adopted for analysis.Results Quasispecies complexity of HBV S gene in chronic HBV carriers and chronic hepatitis B tended lower than that of the severe chronic hepatitis B, but the difference was not of statistical significance (P>0.05).In T cell epitope 45, 47, 85 amino acid sites of the HBV S gene, the constitution of quasispecies in the chronic hepatitis B was more complex than that of the HBV carriers (P=0.01), but compared with the severe chronic hepatitis, the difference was not significant (P=0.06).The computer model showed that both the dominant clones and the non dominant clones could effectively bind to the receptors of cytotoxic T lymphocytes.Conclusion Quasispecies in some T cell epitopes of HBV S gene may be related with the clinical outcome of hepatitis B.
3.The characterization and clinical significance of quasispecies in the reverse transcriptase region of hepatitis B virus in hepatitis B virus infected patients with different disease stages
Bilian YAO ; Feng LIU ; Suyuan HUANG ; Demin YU ; Li CHEN ; Yue HAN ; Xinhua LI ; Xinxin ZHANG
Chinese Journal of Infectious Diseases 2011;29(12):717-722
Objective To characterize the profile and clinical significance of hepatitis B virus (HBV) quasispecies in patients infected with hepatitis B virus based on the sequence of reverse transcriptase (RT) region.Methods Fifty HBV infected treatment-naive patients were enrolled and divided into three groups,asymptomatic carriers (ASC) group (10 cases),chronic hepatitis B (CHB) group (30 cases) and liver cirrhosis (LC) group (10 cases).HBV genomes were extracted from serum samples.The sequence of RT region was amplified by polymerase chain reaction (PCR) and cloned into vectors.Fifteen to thirty clones per sample were selected,sequenced and analyzed by bioinformatics software.The mean values among groups were compared by analysis of variance.The median values among groups were compared by nonparametric statistics.The enumeration data were analyzed by x2 test.Results Totally 1221 HBV RT region nucleotide sequences were obtained (152from ASC patients,780 from CHB patients and 289 from LC patients).Genotype distribution showed no difference among three groups.However,the quasispecies complexity showed significant differences among the three groups,LC group >CHB group> ASC group (F=33.400,P<0.05).The quasispecies diversity was LC group >CHB group> ASC group,and that of LC group was significantly different from the other two groups (F=18.070,P<0.05),while there was no significant difference between CHB and ASC patients.Conclusions The HBV isolated from patients in immune clearance phase have higher variability than those isolated from patients in immune tolerance phase.The longer the infection persists and the more severe the disease is,the more variable HBV quasispecies are.
4.Characteristic of SCL-90 in detoxification addicts in reeducation center
Feng LI ; Huan-guang HUANG ; Yue-han SONG ; Pengcheng ZHOU ; Hehe WEI ; Honggui PAN ; Leilei ZANG ; Jing GUAN ; Jia WANG ; Yongkang TAN ; Zhenhe TIAN ; Xiaolan LIU ; Jianxiong HUANG ; Suyuan XIAO ; Yuan LIANG ; Xue LI
Chinese Journal of Rehabilitation Theory and Practice 2006;12(1):73-74
ObjectiveTo investigate the Character of Symptom Checklist 90 (SCL-90) in detoxification addicts in reeducation center.Methods100 detoxification addicts in reeducation center were evaluated.ResultsThe results showed that the mean scores of all factors in detoxification addicts were higher than those of normal population, and there was difference between different drug dependence addicts.ConclusionThe detoxification addicts shows serious psychological disorders.
5.Effect of Synthesized Rehabilitation on Detoxification Addicts
Feng LI ; Huan-guang HUANG ; Lei-lei ZANG ; Pengcheng ZHOU ; Hehe WEI ; Yuehan SONG ; Jing GUAN ; Jia WANG ; Honggui PAN ; Yongkang QIN ; Zhenhe TIAN ; Suyuan XIAO ; Ping ZHANG ; Xiaolan LIU ; Jianxiong HUANG
Chinese Journal of Rehabilitation Theory and Practice 2006;12(4):347-348
ObjectiveTo explore the effect of synthesized rehabilitation on detoxification addicts.Methods100 detoxification addicts were randomly divided into the rehabilitation group and control group with 50 cases in each group. Cases of the rehabilitation group were treated with "ten-step" rehabilitation treatment, but that of the control group did not. The rehabilitating treatment included cognitive psychotherapy, behavioral therapy, family treatment, social skill training and etc. The effects of two groups were evaluate with Symptom Checklist-90 (SCL-90) scale.ResultsExcept hostility, other factorial scores of the rehabilitation group including positive items, somatization, obsession, interpersonal sensitivity, depression, phobia, paranoid and psychotic factors were significantly higher than that of the control group (P<0.05~0.01).ConclusionThe synthesized rehabilitation can obviously improve the psychology of detoxification addicts.