Cervical cancer (U_(14)) cell surface antigen induced by methylcholanthrene was extracted by 1-butanol. The U_14 cells were incubated in 2. 5% 1-butanol, the resul-ted supernatants were referred to as CBE (crude butanol extract). Protein concentration ofCBE determined by the Lowry assay was 147?g/ml. CBE was characterized by SDS-PAGE, which showed eight bands on gel. Their molecular weight range was found to be18-70 kD. CBE was tested by indirect ELISA, which showed that CBE had positive reac-tion with both monoclonal (AU_(14-1)) and polyclonal antibodies. These results indicatedthat CBE contained low molecular cervical cancer cell surface antigen and the antigenicdeterminant which binds to AU_(14-1) specifically.