Objective To evaluate the clinical value of Rta protein antibody IgG of EB virus(Rta/IgG)in the diagnosis of naso‐pharyngeal carcinoma patients in Zhuhai .Methods A total of 75 patients with nasopharyngeal cancer diagnosed by histopathologi‐cal detection without therapy were recruited into nasopharyngeal cancer group ,100 healthy persons were selected into control group ,there were 19 patients in liver cancer ,gastric cancer ,lung cancer ,lymphoma group and cervical cancer group .Rta/IgG and EB virus antibody of shell (VCA/IgA) were detected by enzyme‐linked immunosorbent assay .Results The positive rate of Rta/IgG in the nasopharyngeal cancer group was significant higher than those of liver cancer ,gastric cancer ,lung cancer ,lymphoma group and cervical cancer and control group(P<0 .05) .The positive rates of Rta/IgG in different clinical stage of nasopharyngeal cancer had no significant differences(P>0 .05) .The sensitivity rates of Rta/IgG single detection ,VCA/IgA single detection ,com‐bined detection of Rta/IgG and VCA/IgA were 81 .3% ,89 .3% ,94 .6% respectively ,while the specificity rates were 93 .0% ,84 . 0% ,80 .0% respectively .Conclusion Rta/IgG is a sensitive and specific serologic parameter for nasopharynx cancer diagnosis ,but it is not a proper maker for evaluating clinical stage of nasopharynx cancer .The combined detection of Rta/IgG and VCA/IgA could improve the diagnosis rate of nasopharyngeal carcinoma in Zhuhai .