OBJECTIVE To study the safe methods of used disposal medical supplies to provide scientific results of decision-making for the health administration. METHODS Using the methods of "standard prevention", "isolated transformation", and "unit calculation", the disposable medical supplies were safely treated in a completely-closed and entirely-programmed way without disinfecting or damaging. The efficacy of the methods was evaluated. RESULTS Form 2006 to 2007, the distribution of disposable medical supplies was 13 306.12 kg and 14 046.00kg and the retrieved quantity was 13 306.00 kg and 14 041.00kg. The improved methods of quantification solved the problem existing in distribution and retrieving of the disposable medical supplies. The safety and convenience of the whole process had been greatly improved. CONCLUSIONS Compared with destroying shapes, disinfecting and "face to face" exchange methods, the methods of using "standard prevention", "isolated transformation" and "unit calculation" are safer, more economic, and more effective.

Objective To investigate the correlation between interferon γ-inducible protein-10 and expression of peroxisome proliferator-activated receptor (PPAR) and fatty acid-binding protein-4 (FABP-4) in pregnant women with preeclampsia. Methods 62 PE pregnant women who were treated in our hospital from January 2016 to January 2017 were enrolled in this study. They were divided into mild PE group (34 cases) and severe PE group (28 cases) according to their severity of disease. In addition, 30 healthy pregnant women in our hospital were selected as the control group. The levels of IP-10 in the three groups were measured by enzyme-linked immunosorbent assay (ELISA), and the levels of PPAR and FABP-4 and the correlation between them were analyzed using western blot assay. Results The levels of IP-10 in mild and severe PE pregnant women were significantly higher than those in the control group, and the mild group was lower than the severe group(P＜0. 05). Compared with the control group, the level of PPAR in mild and severe PE pregnant women decreased significantly, and the severe group was lower than the mild group (P＜0. 05). Compared with the control group, FABP-4 levels in mild and severe PE groups were significantly elevated, and the mild groups was lower than the severe group(P＜0. 05). There was a negative correlation between the level of PPAR and IP-10 (r=-0. 529, P＜0. 05), and a positive correlation between FABP-4 and IP-10(r=0. 692, P＜0. 05). Conclusion There is a certain correlation between PPAR and FABP-4 and the level of IP-10 in pre-PE pregnant women. lt can be used to determine the condition of pre-PE in pregnant women.

Objective: To investigate the role and mechanism of action of Yiqi Huoxue Recipe (YQHXR) in regulating autophagy and reversing liver fibrosis in rats with carbon tetrachloride (CCl4)-induced liver fibrosis. Methods: Healthy male Wistar rats were intraperitoneally injected with a mixture of CCl4 (30%) and olive oil (70%) twice a week for 8 weeks to establish a rat model of liver fibrosis. The rats administered normal diet were used as control group. Furthermore, YQHXR or Fuzheng Huayu Recipe (FZHYR) was intragastrically administered to the rats. The serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured using an automatic biochemical analyzer. Hematoxylin-eosin (HE) staining and Masson staining were performed to observe the degree of fibrosis in rat liver. The protein expression of α-smooth muscle actin (α-SMA) and type I collagen α1 chain (Col1A1) in liver tissue was measured by immunohistochemistry. Furthermore, the mRNA and protein expression of α-SMA, Col1A1, autophagy-related protein 7 (Atg7), microtubule-associated protein 1 light chain 3 (LC3), and ubiquitin-binding protein (SQSTM1/p62) were determined using qRT-PCR and Western blotting, respectively. Comparison between multiple groups was made by one-way analysis of variance, and comparison between any two groups was made using the LSD test. P < 0.05 was considered as statistically significant. Results: The YQHXR group and FZHYR group had significantly lower serum levels of ALT and AST than the model group (ALT: 66.8±10.42 U/L and 73.2±10.33 U/L vs 106.80±18.24 U/L, F = 31.672, P < 0.001; AST: 122.6±16.65 U/L and 125.4±16.92 U/L vs 278.4±66.14 U/L, F = 25.539, P < 0.001). The pathological grades of hepatic fibrosis were S5.64±0.22, S3.70±0.35, and S3.90±0.34 in the model group, YQHXR group, and FZHYR group, respectively (F = 362.188, P < 0.001). Compared with the control group, the YQHXR group and FZHYR group had significantly reduced mRNA and protein expression of α-SMA, Col1A1, Atg7, and LC3B and significantly increased expression of p62 (all P < 0.05), and the differences were greatest in the YQHXR group. Conclusion YQHXR and FZHYR can prevent or reverse liver fibrosis by regulating hepatocyte autophagy and inhibiting hepatic stellate cell activation and collagen deposition.

The access evaluation of new medical technology is an important part of the preclinical application of medical technology and plays a vital role in ensuring the quality and safety of medical services.However,in the con-crete practice of access evaluation,there are still some problems such as imperfect access theoretical framework,imperfect evaluation index system.With the strategic support of health policies,laws,and regulations,the theory and method of HB-HTA are used for reference,core elements such as assessment subject,assessment object,and assessment content are comprehensively considered,the index system is designed from the dimensions of tech-nical characteristics,safety,effectiveness,economy and applicability,and the access evaluation framework of im-ported medical new technologies is constructed.To offer a theoretical framework and evidence-based basis for medi-cal facility medical technology access management.

Objective To investigate the clinical and pathological features of children with glycogen storage disease (GSD). Methods A retrospective analysis was performed for ten children with GSD who were admitted to the Third Hospital of Hebei Medical University and The Fifth Medical Center of Chinese PLA General Hospital from January 2002 to January 2022, based on medical history, liver biochemistry, and liver biopsy, and population characteristics, clinical manifestations, biochemical parameters, and liver histopathological characteristics were compared and analyzed. Results All ten children had developmental retardation and a short stature, with the manifestations of abnormal liver function, mild weakness, poor appetite, yellow urine, and yellow eyes, and four children had hepatosplenomegaly. Among the ten children, six had the clinical manifestations of hypoglycemia, and one had bilateral gastrocnemius hypertrophy and positive Gower sign. Two children had positive CMV IgG. Liver histopathological manifestations included diffuse enlargement of hepatocytes, light cytoplasm, and small nucleus in the middle like plant cells, with or without fibrous tissue proliferation. Conclusion Most patients with GSD have developmental retardation and abnormal aminotransferases, and liver pathological examination shows specific pathological features.