Objective To investigate The effect of TAM(2.5 uM)in the chemotherapy of HCC and its mechanism. Methods An adriamycin-resistant human HCC cell subline(Hep-3B/ADM)was established,through exposure to gradually increased concentration of ADM.Drug sensitivity was measured by MTT.Modulation of ADM-induced apop- tosis of Hep-3B/ADM cells was assessed by Flow cytometry(FCM).PKC activity was determined by applying compet- itive protein binding method.Results TAM(2.5uM )decreased the IC50 of ADM from 1.64?g/mL to 0.23?g/mL in the Hep-3B/ADM cells.TAM exhibited a 2-fold potentiation on apoptosis caused by ADM in Hep-3B/ADM cells.With TAM,PKC activity was lower than that in control groups from 30 seconds to 24 hours.But this effect of TAM could be reversed by the co-treatment of the cells with TPA.Conclusion TAM(2.5 uM)significantly en- hanced ADM-induced cytotoxicity.The mechanism is that TAM potentiates the apoptosis induced by ADM through pro- tein kinase C inhibition.

Objective　To evaluate the status of urological laparoendoscopic single-site surgery (LESS) technique in China.　Methods　We had conducted a systematic review of literatures of urological laparoendoscopic single-site surgery published from Chinese institutions using WanFang,CNKI,VIP,CBM,GoogleScholar and MEDLINE databases.All relevant articles were selected with consensus and analyzed according to the inclusion and exclusion criteria.　Results　Overall,205 articles from 66 different centers all over China published between January 2009 and June 2012 were included in the analysis. Of which,there were 26 articles of science citation index papers and 179 articles from Chinese academic journals.The included literature was mainly composed of case series reports ( 133 articles,64.9％ ),comparison studies (13 articles,6.3％ ),reviews or comments (20 papers,9.8％ ),nursing related papers (28 pieces,13.7％ ),animal surgery studies (6 articles,2.9％ ),and training (5 articles,2.4％ ).A total of 1790 clinical cases were reported during the study period,56.9％ of these were procedures done in the upper urinary tract,only 5.8％ were in the lower urinary tract,and 37.3％ were genital or other procedures.Procedures of tumor-related and reconstruction required accounted for 18.8％ and 18.7％,respectively.Pure single-port technique was applied on 58.3％ of cases.In cases of a single-port platform used,45.6％ used the commercially available devices and 54.4％ used homemade devices.Transperitoneal accesses were adopted in 59.5％ of cases and the other 40.5％ were performed through extraperitoneal approaches.The umbilicus was used as the site of access in 58.5％ of cases.A total of 25 indications were reported,including adrenalectomy,nephrectomy and ureterolithotomy,etc. in the upper urinary tract,radical cystectomy,radical prostatectomy and transvesical enucleation of the prostate,etc.in the lower urinary tract,and varicocelectomy,orchidopexy and others. The three most often done procedures were varicocelectomy (601　cases,33.6％ ),renal cyst decortication (408 cases,22.8％ ) and adrenalectomy ( 179 cases,10.0％ ).The overall conversion rate was 5.5％,and complications were encountered in 3.4％ of cases.　Conclusions Laparoendoscopic single-site surgery has been widely adopted by Chinese urologists and largely covered the spectrum of conventional urologic laparoscopic procedures,with most of these being non-reconstructive operations for the treatment of benign diseases.In large,we are still under the initial stage of LESS application in urology.And more work on creative innovation and well-designed studies are needed to further evaluate its role in the urological minimally invasive surgery.

Objective To investigate the changes and clinical significance of the platelet parameters in tumor patients before or after radiochemotherapy.Methods The platelet parameters(PLT,PCT,MPV,PDW)were detected in 65 cases of tumor patients before and after radiochemotherapy by the Sysmex XN-1000 automatic hemacytology analyzer.Then the detection results were com-pared with those in the control group.Results Compared with the control group,PLT and MPV before therapy in the tumor pa-tients were significantly increased(P <0.01),but PCT and PDW had no statistical differences between the two goups(P >0.05). PLT,PCT and MPV after radiochemotherapy in the tumor patients were significantly lower than those before treated(P <0.01 ), but no statistically significant difference in PDW was found(P >0.05).Conclusion The measuration of platelet parameters in the tumor patients with radiochemotherapy has certain clinical significance to observe the status of the bone marrow suppression and es-timate the state of illness.

Objective To investigate the efficacy of rehabilitation in treatment of patients with hypertensive cerebral hemorrhage. Methods 64 patients with hypertensive intracerebral hemorrhage according to whether the implementation of the rehabilitation treatment of patients were randomly divided into two groups, the observation group and the control group,and each group(32 cases) was observed at two of the limb motor function,activities of daily living and quality of life scores. Results There were high blood pressure in patients with cerebral hemorrhage FuglMeyer score, Barthel index score, QQL quality of life score after 4 weeks after treatment, scores were significantiy improved by the test showed significant difference( all P ＜0. 05). Conclusion Active rehabilitation in patients with hypertensive intracerebral hemorrhage could improve the patients' limb motor function and daily life, improve quality of life of patients.

Objective:To determine whether alpha-fetoprotein(AFP) messenger RNA(mRNA),a specifically hepatocyte-expressed gene,could be a marker of metastasis in hepatocellular carcinoma. Methods:Peripheral blood samples were obtained from 30 patients with hepatocellular carcinoma (HCC).A sensitive reverse transcription polymerase chain reaction( RT-PCR) technique was used to detect the human AFP mRNA in the blood.Results:Of the 30 HCC patients,14(46.7%) patients had positive AFP mRNA.The presence of AFP mRNA correlated with the TNM clinical stages,the size of tumor,extrahepatic metastasis or with portal vein carcinothrombosis(P0.05).Conclusion:The positive of AFP mRNA in the peripheral blood can indicate the presence of hematogenous metastasis in patients with HCC.

Objective To observe the clinical curative effect of baijiang enema liquid combined with special - ray therapeutic apparatus on chronic pelvic infection. Methods 120 cases with chronic pelvic infection were randomly divided into 60 cases in treatment group and 60 in control group. Cases in the treatment group were treated by enema of Baijiang enema liquid combined with special - ray therapeutic apparatus, and cases in the control group were treated by rectal administration of Kangfu Anti - inflammatory Suppository combined with special -ray therapeutic apparatus,with 15 days for a treatment course and 2 courses for treatment. Results The comprehensive effect, TCM syndrome effect and signs effect in treatment group were better than those in control group (P

It was argued whether bisphenol A(BPA) can induce reproductive toxicity to male,so in the present paper the recently published articles on the reproductive toxicity induced by BPA to male were reviewed.It was indicated that BPA has no obvious embryotoxicity,but BPA can directly induce the reproductive toxicity to male reproductive system,and the testis and prostate were the main target organs of BPA.Even low level BPA exposure could produce toxic effects.

Taking the network course of pediatric dentistu-occlusal inducement as an example,this paper put forwards several aspects that should be focused on,including building a strong team to implement the project; taking advantage of muhimedia and providing rich teaching resources in various forms,breaking traditional teaching modes and taking full advantage of the characteristics of the network platform to provide students with individualized teaching service ; building self-regulatory system and real-time interactive platform for teachers and students; establishing long-term mechanism,cultivating a network course reserve team with modernization quality and updating teaching content constantly to let it become an open teaching platform Finally the paper explained the significance of network course construction and promotion.

This paper reports the determination of sex of human blood stains in threecriminal cases by the DNA amplification technique.The results fit the cases de-tails and gave the scientific evidence for the justice.The blood stains were dige-sted with proteinase K.The protein was extracted with the phenol-chloroform.The DNA was precipitated with NaCl and ethyl alcohol.Amplification of DNAwas carried out using two pairs of primer Y1.1 Y1.2 and Alu9.1 Alug.2 and heatstable FD polymerase.The amplified products were subjected to agarose gel(con-taining ethidium bromide)electrophoresis.Sex of blood stains was determinedaccording to the amplified products.PCR technique is rapid to perform,sensitiveand simple.No special equipments and isotope labelled probe are required.