Objective To investigate the relationship between the expressions of TMPRSS4 and clinical pathologi-cal parameters in gastric carcinoma. To explore its significance in judging the prognosis of patients. To analyze the expression of TMPRSS4 in the epithelial-mesenchymal transition. Methods The expressions of TMPRSS4, E-cad-herin and Vimentin were detected by immunohistochemistry ( SABC) in 100 cases of gastric carcinoma specimens and corresponding adjacent normal tissue. Results In gastric carcinoma, TMPRSS4, E-cadherin and Vimentin positive rate was 47%,45%,37%. In adjacent tissues,their positive rate was 9%,30%,4%. The differences be-tween tumor tissues and adjacent tissues had statistical significance ( P<0.05 ) . High expression of TMPRSS4 cor-related significantly with lymph node metastases and TNM stage (P<0.05), but not with gender, age, size, tumor histological type,depth of invasion (P>0.05). The 5-year survival rate of patients with high TMPRSS4 expression was significantly lower than that in patients with low expression. The high TMPRSS4 expression was significantly correlated in gastric carcinoma accompanied by low expression of E-cadherin (rs = -0.207,P=0.038) and high Vimentin expression (rs=0.233,P=0.020). Conclusion The expression of TMPRSS4 is closely related to the biological characteristics in gastric carcinoma,detection the expression of TMPRSS4 is valuable in predicting tumor prognosis,invasion and metastasis. TMPRSS4 may promote invasion, metastasis of human gastric carcinoma through epithelial-mesenchymal transition by reduce the expession of E-cadherin.

OBJECTIVE:To optimize the forming technology of Tongtong granules.METHODS:The category and ratio of the adjuvants were optimized taking moisture absorption percentage as index;the forming technology of Tongtong granules was optimized by orthogonal experiment with forming rate and moisture absorption percentage as indexes and with the ratio of extract to adjuvant,the concentration and amount of ethanol as factors.RESULTS:The optimal ratio of lactose to dextrin was 3:2.The optimal forming technology was as follows:the ratio of extract to adjuvant was 1:2;70%alcohol was used as the wetting agent with its amount at 12%(mL?g~(-1)).The Tongtong granules mobility enjoyed good flowability with a critical relative humidity of about 70%.CONCLUTION:The forming technology is feasible,stable,reasonable and reproducible and it serves as theoretic basis for industrial production.

Objective:To determine whether alpha-fetoprotein(AFP) messenger RNA(mRNA),a specifically hepatocyte-expressed gene,could be a marker of metastasis in hepatocellular carcinoma. Methods:Peripheral blood samples were obtained from 30 patients with hepatocellular carcinoma (HCC).A sensitive reverse transcription polymerase chain reaction( RT-PCR) technique was used to detect the human AFP mRNA in the blood.Results:Of the 30 HCC patients,14(46.7%) patients had positive AFP mRNA.The presence of AFP mRNA correlated with the TNM clinical stages,the size of tumor,extrahepatic metastasis or with portal vein carcinothrombosis(P0.05).Conclusion:The positive of AFP mRNA in the peripheral blood can indicate the presence of hematogenous metastasis in patients with HCC.

Objective To investigate The effect of TAM(2.5 uM)in the chemotherapy of HCC and its mechanism. Methods An adriamycin-resistant human HCC cell subline(Hep-3B/ADM)was established,through exposure to gradually increased concentration of ADM.Drug sensitivity was measured by MTT.Modulation of ADM-induced apop- tosis of Hep-3B/ADM cells was assessed by Flow cytometry(FCM).PKC activity was determined by applying compet- itive protein binding method.Results TAM(2.5uM )decreased the IC50 of ADM from 1.64?g/mL to 0.23?g/mL in the Hep-3B/ADM cells.TAM exhibited a 2-fold potentiation on apoptosis caused by ADM in Hep-3B/ADM cells.With TAM,PKC activity was lower than that in control groups from 30 seconds to 24 hours.But this effect of TAM could be reversed by the co-treatment of the cells with TPA.Conclusion TAM(2.5 uM)significantly en- hanced ADM-induced cytotoxicity.The mechanism is that TAM potentiates the apoptosis induced by ADM through pro- tein kinase C inhibition.

C. Conclusion The optimal SINI TANG composition comprises three active components combined at clinical maximal dosage showed the best therapeutic efficacy, of which A is a key factor and B, C are necessary factors for the composition in SINI TANG.

Objective To compare the effects of hyperbaric oxygenation(HBO) and routine therapy on diabetic foot. Methods Thirty six subjects were randomized to HBO and routine groups. In HBO group,patients stayed in 0.25 MPa HBO chamber for 100 min,wearing masks and inhaling pure oxygen for 60 min,with 10 min interval.This treatment was performed once a day for 10 times as a course,altogether 2 courses for each patient.By means of hemorrheology and electromyograghy (EMG), and the color Doppler ultrasonograghy of lower extremities for detection of the microcirculation states of lower limbs, thirty six patients with diabetic foot were assessed. Results In HBO group all of patients showed that the abnormalities in hemorrheologic, EMG parameters, and the arteria lesions as revealed by Doppler ultrasonograghy were improved more or less compared with before the treatment( P

Taking the network course of pediatric dentistu-occlusal inducement as an example,this paper put forwards several aspects that should be focused on,including building a strong team to implement the project; taking advantage of muhimedia and providing rich teaching resources in various forms,breaking traditional teaching modes and taking full advantage of the characteristics of the network platform to provide students with individualized teaching service ; building self-regulatory system and real-time interactive platform for teachers and students; establishing long-term mechanism,cultivating a network course reserve team with modernization quality and updating teaching content constantly to let it become an open teaching platform Finally the paper explained the significance of network course construction and promotion.

The effects of Panax notoginseng saponins (PNS) and verapamil(Ver)on hemo-dynamics of internal carotid artery and superior mesenteric artery were determined and compared in anesthetized Sprague ~ Dawley strain rats. PNS 30-150 mg ? kg-1 iv was shown to reduce the mean blood pressure, internal carotid arterial resistance and superior mesenteric arterial resistance by 11 % ~19%, 14%~27%,23%~ 39%respectively. Meanwhile, the mesenteric ar-terial blood flow increased.by 16%~51%. Ver showed similar reflects as PNS. The results indicated that the vasodilation effects of PNS and Ver are vascular selective, that is PNS and Ver are more potent in dilating the mesenteric artery than the internal carotid artery.

Objective　To evaluate the status of urological laparoendoscopic single-site surgery (LESS) technique in China.　Methods　We had conducted a systematic review of literatures of urological laparoendoscopic single-site surgery published from Chinese institutions using WanFang,CNKI,VIP,CBM,GoogleScholar and MEDLINE databases.All relevant articles were selected with consensus and analyzed according to the inclusion and exclusion criteria.　Results　Overall,205 articles from 66 different centers all over China published between January 2009 and June 2012 were included in the analysis. Of which,there were 26 articles of science citation index papers and 179 articles from Chinese academic journals.The included literature was mainly composed of case series reports ( 133 articles,64.9％ ),comparison studies (13 articles,6.3％ ),reviews or comments (20 papers,9.8％ ),nursing related papers (28 pieces,13.7％ ),animal surgery studies (6 articles,2.9％ ),and training (5 articles,2.4％ ).A total of 1790 clinical cases were reported during the study period,56.9％ of these were procedures done in the upper urinary tract,only 5.8％ were in the lower urinary tract,and 37.3％ were genital or other procedures.Procedures of tumor-related and reconstruction required accounted for 18.8％ and 18.7％,respectively.Pure single-port technique was applied on 58.3％ of cases.In cases of a single-port platform used,45.6％ used the commercially available devices and 54.4％ used homemade devices.Transperitoneal accesses were adopted in 59.5％ of cases and the other 40.5％ were performed through extraperitoneal approaches.The umbilicus was used as the site of access in 58.5％ of cases.A total of 25 indications were reported,including adrenalectomy,nephrectomy and ureterolithotomy,etc. in the upper urinary tract,radical cystectomy,radical prostatectomy and transvesical enucleation of the prostate,etc.in the lower urinary tract,and varicocelectomy,orchidopexy and others. The three most often done procedures were varicocelectomy (601　cases,33.6％ ),renal cyst decortication (408 cases,22.8％ ) and adrenalectomy ( 179 cases,10.0％ ).The overall conversion rate was 5.5％,and complications were encountered in 3.4％ of cases.　Conclusions Laparoendoscopic single-site surgery has been widely adopted by Chinese urologists and largely covered the spectrum of conventional urologic laparoscopic procedures,with most of these being non-reconstructive operations for the treatment of benign diseases.In large,we are still under the initial stage of LESS application in urology.And more work on creative innovation and well-designed studies are needed to further evaluate its role in the urological minimally invasive surgery.

Objective To compare biomechanical performances of parallel plate fixation and vertical plate fixation for better clinical treatment of humeral intercondylar comminuted fractures (HICF).Methods Models of T-shaped fracture were created using an osteotome at the distal end of humerus in 6 specimens of upper limb in 3 human adult cadavers.The 2 fracture models of left and right humeri in the same cadaver were respectively fixated with parallel plating and vertical plating.On a biomaterial-testing machine,a vertical load was applied onto the fixated distal humerus at a speed of 5 N/s till it reached 2000 N.The loading was repeated 3 times.Vertical compression displacement,intercondylar separating displacement,deformation of medial and lateral condyles and vertical compression stiffness were documented for statistic analyses. Results Under the same load,parallel plating led to significantly less compression displacement,separating displacement and deformation of condyles( P ＜ 0.05) but significantly greater axial compression stiffness( P ＜ 0.05) than vertical plating. Conclusion In biomechanics,parallel plate fixation has better performance than vertical plate fixation for HICF.