Two kinds of mouse monoclonal antibodies which recognize distinct epitopes of the IL-2 receptor (a chain) were used to establish a sandwich ELISA for measuring soluble IL-2 receptors. The results indicated that this method is sensitive and specific for detecting soluble IL-2 receptors of cell culture supernatant and serum from both healthy donor and patients with leukemia. Therefore this sandwich ELISA method is useful for basic and clinic immunology research.

We obtained human lymphoblastoid B cells secreting human antibodies IgM and IgG to HFRS virus by Epstein-Barr virus(EBV)transfomation of PBMC.Conditioned media from the supernatant of a human fibroblast cell CRL1506 culture and irradiated CRL1506 cells as feeder cells could markedly promote the growth and antibody produotion of the human B lymphoblastoid cells at low cell density.It was shown that conditioned media contained a high level of interleukin 6(IL-6) .These results would be very useful for human monoclonal antibody technique including improving human B lymphoblastoid cell growth at low cell density and enhancing cloning efficiency andantibody production.

To study the changes in vestibular function in Parkinson's disease patients, and its significance, eletronystagmography (ENG) was used to examine 54 patients with Parkinson's disease, and also 20 hea thy elderly subjects. The results of vestibular function tests including sensory organization test, nystagmus test, cold caloric test and positional test of these two groups were recorded for statisticed analysis. It was found that the rate of vestibular dysfunction in Parkinson's disease patients were 81 4%, and that of the controls 30%. There was significant difference between the two groups ( P

The characteristics of master dissertation topics in medical library and information science and their se-lection methods were analyzed with the dissertations published since 2004 as the study object.The challenges and development trend in training of qualified personnel of medical library and information science were summarized , which may provide certain evidence for the training of masters in medical library and information science .

Most anticancer drugs inhibit tumor cell growth by inducing apoptosis.Apoptosis-evasion,also enhancement of anti-apoptosis ability(e.g.anti-apoptosis related genes over-expression or pro-apoptotic related genes loss,etc.)may lead to tumor cell multidrug resistance.Many researches show that apoptosis-evasion is one of the important factors resulting in tumor multidrug resistance.

Objective To investigate the effect of arthroscopic arthroplasty on the short-term efficacy and stress factors in patients with primary osteoarthritis of the elbow and to guide the clinical application.Methods From February 2013 to February 2016,32 patients with primary primary osteoarthritis of the elbow underwent arthroscopic arthroplasty in Xiaogan Hospital Affiliated to Wuhan University of Science and Technology.The levels of serum TNF-α,IL-6,IL-10,adrenocorticotrophic hormone(ACTH) and other stress factors were measured before and after the operation.The ROM,MEPS and VAS were used to evaluate the curative effect before,3 months and 6 months after operation.Results All patients were followed up successfully.The excellent rate was 96.9%(31/32) 1 year after the operation.Preoperative ROM,MEPS score,VAS score were (83.2±18.6) °,(63.2±14.5) points,(3.2±1.8) points;3 months,6 months postoperative ROM were (102.3±18.9) °,(124.3±15.4) °,and MEPS scores were (75.4±16.8) points and (89.5±14.3) points respectively,which were significantly higher than those before operation (ROM F value of 43.19,P<0.001;MEPS F value of 23.86,P<0.001).Three months,6 months postoperative VAS scores were (0.5±0.2) points and (0.3±0.1) points,which was significantly decreased(76.55,P<0.001);preoperative TNF-α,IL-6I,L-10,ACTH were respectively (12.5±2.5) μg/L,(5.4±2.3) ng/L,(10.5±4.0) ng/L,(22.4±6.3) ng/L,and were (38.4±8.6) μg/L,(25.6±8.4) ng/L,(23.1±4.8) μg/L,(58.6 ± 15.0) μg/L 1 day after surgery,which significantly increased compared with preoperative levels(P<0.05);1 week after patient TNF-α,IL-10 were respectively (9.4±1.2) μg/L,(7.5±3.5) ng/L,which decreased significantly(P<0.001).IL-6,ACTH were respectively (3.1±0.9) μg/L,(19.5±5.8) ng/L,which showed no significant difference(P>0.05).Conclusion For the modest primary osteoarthritis of the elbow,arthroscopy arthroplasty can achieve good therapeutic effect,improve the patient′s motor function and life quality,and minimally invasive surgical approach is conducive to relieve systemic stress.

[Objective] Analyze the academic thought ofpurge stomach and tonify spleentreatment used by Professor SUN Zhi in acupuncture on type-2 diabetes mellitus during his clinical experience.[Method] According to new recognition of type-2 diabetes mellitus pathogenesis, study of acupuncture theory, clinical manipulation, conclude acupuncture point choices and needling methods ofpurge stomach and tonify spleentreatment, studying its characteristics. [Result] Type-2 diabetes mellitus belonging toXiaokecategory which features excessive discharge of urine and persistent thirst in Chinese medicine, but their pathogenesis is not the same, Professor SUN Zhi based on years of clinical observation found that patients with type-2 diabetes mellitus commonly manifested obese, polyorexia and accompanied by fatigue, summarized asstrong stomach and weak spleen. Tonify the deficiency and purge sthenia, using acupuncture manipulation, regulating spleen and stomach function, by reducing method of acupuncture Zhongwan, Zusanli and Neiting to inhibt stomach and control appetite, reinforcing method of acupuncture Sanyinjiao, Taibai, Pishu to enhance the transport of the spleen, meaning purging stomach and tonifying spleen. This treatment of type-2 diabetes mellitus in clinic achieved good results. [Conclusion]Purge stomach and tonify spleentreatment offers a new way of acupuncture to treat diabetes, deserving further exploration, popularization and application.

The change of lung lymph after endotoxin-induced lung injury was studied using a sheep model with chronic lung lymph fistula. After endotoxin injection Ppa、PMV、 Q_L、CL_p、L_A/P_A、and Kf all increased markedly. The ratios of effects of pressure and permeability on Q_L were 1:0.53 and 1:2.31 during phase I and phase H respectively. The results indicated that the formation of pulmonary edema after injury related mainly to the increases of PMV and Kf and to the decrease of effective colloid osmotic pressure. The PMV was the main factor of formating edema during phase I and the permeability was that during phase Ⅱ.

Tumor growth and migration are correlated with continuous neovascularization .Slit2-Robo1 signal pathway initially positioned in the nervous system plays an important role in the growth and neuronal axon guidance during migration.However,vascular system resembles the structural features and growth mechanism of neural system .Recent researches have shown that Slit 2-Robo1 signaling pathway plays an important role in vas-cular system,especially in tumor vessel .And Slit2-Robo1 has been shown to play a role in brain metastasis in breast cancer and supporting tumor growth in melanoma .Therefore,the role of Slit2-Robo1 in tumor growth and migration depends on the cancer type .Currently ,researches in this field show controversial results .And the status of Slit2-Robo1 signaling pathway in lung cancer is reviewed in this essay .

OBJECTIVE:To establish the quality standard for Chaige ganmao mixture. METHODS:TLC was conducted to iden-tify the Scutellaria baicalensis and Paeonia lactiflora;HPLC was conducted to determine the content of puerarin. The column was Zorbax Eclipse XDB-C18 with mobile phase of methanol-water(25:75,V/V)at flow rate of 1.0 ml/min,detection wavelength was 250 nm,and column temperature was 25℃,and the injection volume was 5μl. RESULTS:TLC showed clear spots and good sepa-ration. The linear range of puerarin was 18-198 μg/ml(r=0.999 9);RSDs of precision,stability and reproducibility tests were low-er than 2%,recovery was 99.04%-99.75%(RSD=0.28%,n=6). CONCLUSIONS:The standard can be used for the quality con-trol of Chaige ganmao mixture.