Objective To investigate the effects of progesterone on apoptosis and Survivin expression protein of Ishikawa cell line of human endometrial carcinoma in vitro.Methods The proliferation of Ishikawa cells in vitro was determined by methyl thiazolyl terazolium (MTT) medhod.Cell cycle,apoptotic percentage were detected by cytometer. The expression of Survivin protein of Ishikawa cells was detected by immunocytochemistry. Results Progesterone inhibited the growth of Ishikawa cells significantly when the concentration of progesterone reached at 0.5?10-5～10-4mol/L(P

Objetive:To detect the gene mutation of a patient with anhidrotic ectodermal dysplasia. Methods: Genomic DNA was extracted from the peripheral blood of the patient and all the exon fragments were obtained by PCR. These fragments were sequenced directly. Results: In EDA gene a novel base transition was detected: -48A→G. Conclusion: There is a new base transition in EDA gene in this patient

Objective To evaluate the efficiency of microsurgical methods for treatment of hypothalamic gliomas in children and the management of postoperative complications Methods Twenty-one tumors were classified into two types according to their location, size and extensive direction The tumors were removed with microsurgical technique by various approaches, such as transcallosal-interforniceal or translongitudinal fissure, transubfrontal methods Results Twenty-one cases of hypothalamic gliomas were verified by surgery and pathology The total and subtotal removal was 18 cases Partial removal was 3 cases There was no mortality in this series Major neurological complications included diabetes insipidus, electrolyte disorder and hyperthermia Surgical complications included subdural fluid collections, hydrocephalus, epilepsy Seventeen cases were followed up, and showed satisfying results Conclusion Microsurgical operations through appropriate approaches and reasonable management of complications are important methods for improving the outcome of hypothalamic gliomas in children

Objective To study the clinical results of grafting of vascularized fibular and ilium to repair bone defects of limbs. Methods From January 1992 to January 2004, 49 cases of bone defects of limbs were treated with vascularized fibular grafting (33 cases) and vascularized iliac grafting (16 cases). Their limb functions were graded according to Ennekings system. The changes of the vascularized fibular and iliac grafts were evaluated radiographically according to the International Symposium on Limb Salvage. Results All the patients were followed up for 1 to 12 years. In the vascularized fibular group, the recovery rate for the operated limbs was 82.7%, the average healing time of the bone was 16.8 weeks and the healing rate of the bone was 87.9%. In the other group, the recovery rate of the operated limbs was 88%, the average healing time of the bone was 15.2 weeks and the healing rate of the bone was 93.8%. Conclusion The vascularized fibular graft can be adopted to repair long bone defects of lower limbs and distal radius, while the vascularized iliac graft can be adopted to repair the bone defects of upper limbs.

BACKGROUND: Recently, it is investigated that shell of plantain seed is a soluble dietary fiber which can be added into foods to regulate content of cholesterol.OBJECTIVE: To investigate the interventional effect of plantain seed on lipid and its lipid peroxidation in rats with hyperlipidemia.DESIGN: Completely randomized grouping design and controlled animal study.SETTING: Laboratory of Pharmacology and Toxicology for New Drug in Hebei Province.MATERIALS: ① A total of 24 healthy SD rats, of grade I, aged 60-70 days, weighting (210±22) g, of either gender, were selected in this study. ② Basic feed was provided by Experimental Animal Center in Hebei Province,and the fractional mass of each component was mentioned as following:flour 0.25, bran 0.1, corn dust 0.22, bean cake 0.22, fish dust 0.02, bone dust 0.02, grass dust 0.05, salt 0.01, yeast dust 0.02, and sunflower seed 0.03. High fat feed was provided by Experimental Animal Center in Hebei Province, and the fractional mass of each component was mentioned as following: basic 0.9, cholesterol 0.015, lard 0.08, and hyocholic salt 0.003.③ Lipid kit was provided by Baoding Changcheng Clinical Reagent Company, and kits of superoxide dismutase (SOD), catalase (CAT),glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) were provided by Nanjing Jiancheng Bioengineering Institute.METHODS: The experiment was completed at the Laboratory of Pharmacology and Toxicology for New Drug in Hebei Province from June to December 2004. ① All 24 rats were randomly divided into 3 groups:normal control group, model group and plantain seed group with 8 in each group. Rats in the normal control group were fed with basic feed. Rats in plantain seed group were fed with high fat feed + 15 g/kg plantain seed and drank routinely. Experimental rats were fed in cages, respectively.Each one was fed with 25 g/d food and drunk freely. The experimental cycle was 12 weeks. ② At the end of experiment, rats were anesthetized to assayed levels of serum triacylglycerol (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), serum SOD and MDA, activities of CAT and SOD in myocardial tissue, content of MDA, and activities of CAT and GSH-Px in hepatic tissue with related kits. ③ Measurement data were compared between each two group with t test.MAIN OUTCOME MEASURES:Comparison of serum lipid level and anti-oxidation among groups at 12 weeks after modeling.RESULTS: All 24 rats were involved in the final analysis. ① At 12 weeks after modeling, activities of SOD in serum and myocardial tissue were lower in model group than those in normal control group and plantain seed group (P ＜ 0.05), but levels of MDA in serum and myocardial tissue were higher in model group than those in normal control group and plantain seed group (P ＜ 0.05). ② At 12weeks after modeling, activities of CAT and GSH-Px in serum and myocardial tissue were lower in model group than those in normal control group and plantain seed group (P ＜ 0.05). ③ At 12 weeks after modeling, levels of TC and TG in serum were higher in model group than those in normal control group and plantain seed group (P ＜ 0.05), but level of HDL-C and ratio between HDL-C and TC in serum were lower in model group than those in normal control group and plantain seed group (P ＜ 0.05).CONCLUSION: Plantain seed at dosage of 15 g/kg can decrease content of lipid and strengthen anti-oxidation of economy in rats with hyperlipidemia.

BACKGROUND: Great concerns haven been given increasingly on inhibition of nutrient with antioxidation efficacy on lipid peroxidation and its effect on prevention of cardiac vascular disease.OBJECTIVE: To probe into the effects of plantain seed that acts on eliminating oxygenic free radical and antioxidation on lipid metabolism and antioxidation in rats.DESIGN: Randomized control experiment was designed.SETTING: Experimental Room of Pharmacology and Toxicology of New Drug in Hebei Province.PARTICIPANTS: The experiment was performed in Experimental Room of Pharmacology and Toxicology of New Drug in Hebe Province from January to December 2004, in which, 40 SD rats were employed, provided from Hebei Experimental Animal Center, of healthy grade I, mass weighted (210±22) g and of either sex. They were randomized into 5groups, named, blank control, positive control, low dosage experiment group, moderate dosage experiment group and high dosage experiment group, 8 rats in each one.METHODS: In blank control, the rats were bred everyday with basic forage that was tallied with AoAc animal nutrient criteria and they were free of drinking. In positive control, the rats were bred with high-lipid forage and free of drinking. In the groups of low, moderate and high dosages of plantain seed, the rats were bred with 2.5 g/kg, 5 g/kg and 15 g/kg plantain seed successively besides high-lipid forage and they were free of drinking. The weight was measured and the food intake was recorded every week. Fasting blood was collected to check total cholesterol in serum once every two weeks. The experiment was end in 12 weeks. Under anesthesia,the blood was collected from hypogastric aorta to check the level of serum blood lipid and the activities of superoxide dismutase (SOD) and catalase.After blood collection, the heart and liver were extracted immediately for management to measure SOD activity and content of lipid peroxide (LPO)in myocardial tissue and the activities of catalase and glutathione peroxidase in liver tissue.MAIN OUTCOME MEASURES: Level of blood lipid and activities of SOD and catalase in rats.RESULTS: Forty rats were employed and all entered result analysis. [1]Serum total cholesterol: It was lower significantly in high dosage group compared with positive control [(1.40±0.13, 1.83±0.13) mmol/L, P ＜ 0.05].[2] Serum SOD activity: It was lower remarkably in positive control compared with blank control [(174.29±10.33, 193.19±.7813) NU/mg, P ＜ 0.05].[3] LPO content in serum: It was higher significantly in positive control compared with blank control [(3.64±0.26, 2.91±0.50) mmol/mg, P ＜ 0.05]and it was lower significantly in moderate dosage group compared with positive control (3.13±0.26, 3.64±0.26, P ＜ 0.05). [4] Activity of catalase in liver tissue: It was lower remarkably in positive control compared with normal control (34.64±3.26, 44.72±2.67, P ＜ 0.05) and it was higher remarkably in moderate dosage group compared with positive control (44.84±3.79,34.96±3.64, P ＜ 0.05).CONCLUSION: Plantain seed reduces the levels of total cholesterol,triglycerin (TG) and LPO in serum and increases SOD activity. At the concentration of 15 g/kg, plantain seed acts most remarkably on eliminating oxygenic free radical and antioxidation and alleviates lipid metabolic disturbance.

Objective To assess the application of IS6110-restriction fragment length polymorphism (RFLP)and Spoligotyping in the epidemiology of tuberculosis and to discuss the characteristic of the Mycobacterium tuberculosis in different regions in China.Methods 158 isolates of Mycobacterium tuberculosis strains, were verified by IS6110-RFLP and spoligotyping.Results The discriminatory power of IS6110-RFLP was higher than that of spoligotyping. The result of spoligotyping was compared with the international spoligotype database. Fourteen types belonged to the shared types, in which the type 1 was epidemic and widely,called Beijing genotype. There was significantly difference among the mycobacterium tuberculosis between Guangdong and other regions in clustered rate and the proportion of Beijing genotype (P

Objective:To investigate the effect of cyclopamine on growth and proliferation of human mammary carcinoma cell line Bcap-37.Methods:The Bcap-37 cells were incubated with cyclopamine,which is the specific inhibitor of Hedgehog signaling pathway.Then the inverted microscope was used to observe the morphologic changes of the cells,and MTT assay,BrdU incorporation and cell cycle analysis were used to examine the influence of cyclopamine on Bcap-37 cell growth and DNA synthesis.Results:Compared with the control group,after the cells were incubated with cyclopamine,obvious morphologic changes of Bcap-37 cells were observed;the growth of Bcap-37 cell was inhibited in a dose and time dependent manner;the DNA synthesis of Bcap-37 cells was obviously inhibited;the number of Bcap-37 cells in S phase decreased and the cells were blocked in G2 phase.Conclusion:Cyclopamine can effectually inhibit the growth and proliferation of Bcap-37 cells,which indicates that Hedgehog signaling pathway may be inappropriately activated in breast cancer and inhibiting Hedgehog signaling pathway can be a useful method in breast cancer treatment.

Objective To establish multiple short tandem repeat (STR) amplification by fluorescence labeling polymerase chain reaction (PCR) for monitoring the effect of allogeneic hematopoietic stem cell transplantation .Methods Sixty‐five patients were analyzed .DNA from peripheral blood of donors and recipients in pre transplantation and post transplantation were extracted , 15 STR loci and sexual loci were amplified by PCR .Results After allo‐HSCT ,54 patients obtained type of donors ,but 3 patients did not ;eight patients showed mixed chimerism .Two cases of type of donor converted into mixed chimerism and two cases of mixed chimerism converted into type of donors after some time .The time of earliest detection ,duration and transformation of each state was different .The earliest detection showed on the 16th day after surgery ,and the last one showed five months later .As to the du‐ration ,the shortest and longest were two months and seven months ,respectively .Conclusion The key factors that significantly in‐fluence the application of STR‐PCR in monitoring the effect of allogeneic hematopoietic stem cell transplantation were samples ,STR loci and proper monitoring time .So to establish the suitable method for this region could truly take maximum advantage of the PCR‐STR method .While a appropriate detecting time and STR loci should be chose .

Objective: To investigate the influence of histamine content in nasal mucosa on blood flow. Methods: Sixty guinea pigs were randomly devided into control group and allergized group and guinea pigs were sacrificed before nasal challenge with allergen and immediately after nasal challenge, at 24, 48, 72 h. The content of histamine in nasal mucosa was examined. Nasal mucosa blood flow was examined in all guinea pigs before sacrifice. The data were analyzed using linear correlation and linear regression. Results: As compared with normal guinea pigs, the content of histamine and blood flow in nasal mucosa of allergic rhinitis guinea pigs were significantly increased before challengd with allergen( P