1.Establishment of the endometriosis rat model in intimal window period
Fang CAO ; Xiaoyang HUANG ; Sumei GE ; Ke XU ; Li CHEN
Chongqing Medicine 2015;(27):3805-3807
Objective To research the endometrial receptivity by established the endometriosis rat model in intimal window period.Methods The autologous endometrial tissues of 22 sexually mature SD rats were transplanted into the homonymy abdomi-nal wall and offside uterine mesometrium.After four weeks,we randomly dissected two rats and observed the growth of etopic en-dometrium.The EMs rats were mated with male rats in the same cage and vaginal plug was observed in the next day.The histomor-phology of eutopic and ectopic endometrial was observed in the fifth day of pregnancy.Results Ectopic endometrium was grown in the abdominal wall and mesometrium,which formed a small bulge cystic mass in 20 rats.All foci was showed adhesion to the sur-rounding tissues in a different extent.In the light microscope,the morphology of ectopic endometrial was as the same as nomal en-dometrium.The establishment of the endometriosis rat mode is successful.Vaginal plug was positive the next day.Nonoperative side uterine was full of fluid in the fifth day of pregnancy.Eutopic and ectopic endometrium were in the secretory phase..Conclusion It is a successful method to establish a rat model of endometriosis by autotransplantion of endometrium.The endometrium of EMs model rats is in embryo implantation window period,and it provides a convenient model for studying endometrial receptivity.
2.Diagnosis and treatment of acute pancreatitis complicated with splenic infarction and literature review
Ying LONG ; Lijuan WEI ; Sumei QIN ; Ge ZOU ; Liang MO ; Huaiyang CAI
Journal of Chinese Physician 2021;23(12):1814-1817
Objective:To investigate the etiology, pathogenesis, clinical manifestations, and treatment of acute pancreatitis (AP) complicated with spleen infarction.Methods:A case of AP combined with splenic infarction was retrospectively analyzed and the literature was reviewed.Results:AP complicated with splenic infarction suggested that the patient′s condition was serious. The impairment of splenic immune function caused by splenic infarction may be an important cause of severe acute pancreatitis (SAP). Early detection and active treatment can obtain a good prognosis, and anticoagulant or antiplatelet aggregation treatment was not the key treatment.Conclusions:AP combined with splenic infarction may be an risk factor for SAP.
3.Method Improvement for Content Determination of Astragaloside Ⅳ in Xiangju Granules and Its Consistency with the Com- ponents of Original Formulation
Chenxi SUN ; Ding GE ; Sumei WANG ; Kang GUO ; Jutao WANG
China Pharmacy 2019;30(14):1914-1919
OBJECTIVE: To improve the method for the content determination of astragaloside Ⅳ in Xiangju granules, and to evaluate the consistency of relevant preparations with the components of original formulation, so as to provide evidence for the modern preparation of TCM compound. METHODS: HPLC-ELSD method was established for the content determination of astragaloside Ⅳ in Xiangju granules, and compared with original standard TLC scanning. Using critrinin, ferulic acid, calycosin glucoside, liquiritin, glycyrrhizic acid, rosmarinic acid, buddleoside and magnoline as control, HPLC method was used to determine the release components of self-made Xiangju granules, Xiangju capsules, Xiangju tablets in water. Fingerprint characteristics chromatogram of different Xiangju preparations and original formulation extract were compared by using Similarity Evaluation System for Chromatographic Fingerprint of TCM (2012 version). At the same time, HPLC-ELSD method was used to determine and compare the release rate of astragaloside Ⅳ from different Xiangju preparations and original formulation extract in water. RESULTS: Established HPLC-ELSD method was specific. The linear range of astragaloside Ⅳ was 0.13-2.10 mg/mL. RSDs of precision, repeatability and stability tests were all lower than 3% (n=6), and average recovery was 97.66% (RSD=1.01%,n=6). Average content of astragaloside Ⅳ by this method was 0.398 mg/g (RSD=1.01%, n=3), which had better reproducibility than TLC scanning. The comparative results of characteristic fingerprints showed that the similarity among Xiangju granules, Xiangju capsules, Xiangju tablets and the original formulation dry extract powder was more than 0.850. Average release rates of astragaloside Ⅳ in Xiangju granules, Xiangju capsules, Xiangju tablets and the original formulation extract were 0.392, 0.358, 0.349, 0.389 mg, respectively. Compared with original formulation extract, there was no statistical significance in release rate of astragaloside Ⅳ in Xiangju granules (P>0.05), while there was statistical significance in Xiangju capsules and Xiangju tablets (P<0.01). CONCLU- SIONS: Established HPLC-ELSD method is accurate and feasible, and is suitable for the content determination of astragaloside Ⅳ in Xiangju granules. The main components of Xiangju granules are consistent with original formulation.