Objective To discuss the effect of quick freezer equipment and quick freezing time on the preparation of cryoprecipitate.Methods The cryoprecipitate,already prepared,was placed into the MBF21 freezer and minus 30℃ SANYO refrigerator for 30-minute and 50-minute storage.The activity of coagulation factor VⅧ,and the content of fibrinogen in different equipment were detected by automatic coagulation analyzer,in order to make sure whether it met the quality requirements.Results The content of fibrinogen in the cryoprecipitates from both of the equipment after 30-minute storage met the requirement,with the qualification rate of 100％.As to the activity determination of coagulation FⅧ,low temperature refrigerator showed a qualification rate of 62.5％,which was significantly lower than that of quick freezer with a qualification rate of 97.5％ (P＜0.01).The cryoprecipitates from both of the equipment after 50-minute storage,with part of fibrin precipitation,had a qualification rate of fibrinogen content lower than 50％ (P＞0.05).The qualification rate of quick freezer and low temperature refrigerator was 35％ and 12.5％,respectively,with significant difference.Conclusion Quick freezer can make the cryoprecipitate quick-frozen,which can ensure the quality.The coagulation factor VⅧ is unstable,whose activity decreased with the increase of temperature.We should try our best to shorten the time off the cold chain.

Objective To investigate the prognostic factors in acute myeloid leukemia subtype M2 (AML-M2) patients with chromosome translocation of 8 and 21. Methods By using G-banding analyses karyotype and combining the clinical data, prognostic factors in 94 cases of de novo adult AML-M2 in our hospital from 2001 to 2007 were retrospectively analyzed. Results Chromosome 8 and 21 translocation were identified in 53.2 % (50/94) of AML-M2 cases. In the patients with other aberrations in addition to t(8;21), their complete remission (CR) rates, overall survival (OS) was lower than the patients with sole t (8;21) and normal karyotype(P <0.05). And the patients with sole t (8;21) whose CR rates and OS had no difference with patients with normal karyotype (P>0.05). The patients were divided into 3 subgroups (low index, less than 2.5;intermediate index, 2.5-20;high index, 20 or more) according to WBC index. The CR had no difference among the 3 subgroups, but the OS of the 3 subgroups was different (P<0.05). The OS in the lowest index group was longer than that in the others. Conclusion Cytogenetically, 53.2% cases had chromosome 8 and 21 translocation, 46 % cases had t(8;21) with additional chromosomal abnormalities, and the main additional abnormalities were loss of a sex chromosome (LOS). t(8;21) AML-M2 patients with additional chromosome abnormalities had low complete remission rate and shorter survival time, and its prognosis was poorer. WBC index have no influence on complete remission rate but effected the survival time.

Objective To explore the relationship between tongue conditions and hemorrheology in acute stage of cerebral hemorrhage and to increase the accuracy of tongue conditions for the syndrome differentiation of acute stage of cerebral hemorrhage.Methods Eighty-eight qualified patients were enrolled into this study.The tongue conditions(tongue color,tongue coating and tongue shape) and hemorrheological indexes(whole blood viscosity,whole blood reduced viscosity,plasma viscosity,hematocrit and fibrinogen) were examined,and their relationship was also analyzed.Results The changes of tongue shape was obvious and low-shear whole blood viscosity was increased in the swollen tongue group(P

Objective To assess the molecular biological characteristics of occult hepatitis B virus (HBV) infection in blood donors in Quzhou.Methods A total of 24 178 blood samples from the free-of-charge blood donors were enrolled in the study,all of donations were tested by two different HBsAg ELISA.The occult HBV infection (OBI) was determined by DNA analysis among the HBsAg negative samples.DNA sequencing and mutational analysis were further performed in the HBV DNA positive samples.The overall situation of occult HBV infection was hereby evaluated and the possible underlying mechanisms discussed.Results Among the 24 020 HBsAg negative samples out of 24 178 blood donors,15 were HBV DNA positive.The occult HBV infection rate was 0.62‰ (15/24 020).Among the 15 samples,9 were genotype C (60％) and 6 genotype B (40％).In addition,a specific mutation in "a" epitope was observed in 11 OBI virus strains by amino acid analysis.Conclusion There was occult HBV infection among blood donors in Quzhou,which is probably associated with the gene mutation of HBV virus.