Methods: The experimental brain injury was induced in the SD rats by free falling object, and the expression of intercelluar adhesion molecule-1(ICAM-1)in the microvascular endothelial cells (EC) of rat brain was examined by immunohistochemistry and S-P histochemical methods.The rats were allocated to group A(normal blank control group), group B (administration of Borneolum), group C(brain injured model group) and group D(model rats treated with Borneolum).Results:ICAM-1 seldom expressed or never expressed in the normal rats brain,the administration of Borneolum could not induce its expression,and ICAM-1 expression increased in the injured brain(P

Objective: To study the effect of Fructus Psoraleae on antipyrine elimination in rats. Methods:After the medication of Fructus Psoraleae decoction once daily for 6 days in a dose of 5. 3 g/kg, antipyrine was fed to rats in a dose of 400mg/kg. Concentration of antipyrine in various time was measured by HPLC, and the pharmacokinetics parameters of antipyrine elimination was caculated. Results: The elimination rate constant (Kel) of antipyrine in rats fed with Fructus Psoraleae decoction was 1.55 times more than that in the control group, and the elimination half - life (t1/2) was shortened by 60. 7 % . Conclusion: Fructus Psoraleae decoction accelerate the elimination of antipyrine in rats by inducing the activities of hepatic oxygenases.

Objective To establish a HPLC fingerprint analysis method of salt-prepared Cortex Phellodendri. Methods C18 column was used, with gradient elution by the mobile phase consisted of acetonitrile-0. 1% phosphoric acid (contained 0. 2 % triethylamine). The detection wavelength was at 230 nm, and the flow rate was at 0. 8 mL/min. Results Seventeen char-acteristic peaks were selected and the fingerprints on HPLC was set up. Conclusion HPLC fingerprint method is repro-ducible, accurate and stable, and can be used for the quality control of salt-prepared Cortex PheUodendri.

Objective To develop a high performance liquid chromatography-ultraviolet detection(HPLC-UV) method for the determination of midazolam in rat plasma,and to study the pharmacokinetics of midazolam in single dose intravascular administration in rats.These results can provide a methodological reference for evaluating cytochrome P450 3A(CYP3A) activity.Methods Firstly,common carotid artery(CCA) intubate was set in rats,Midazolam was injected into rat vena caudalis and plasma samples were collected in different time.Then,the sample was extracted using dichlormethane and evaporated to dry thoroughly with N2 soft stream at 37 ℃.Finally,the residues were reconstituted with 150 ?L 30 %methanol and further analyzed by HPLC.A Phenomenex C18 column(4.6?150 mm i.d,5 ?m)was employed at 20 ℃.The mobile phase consisted of(A) methanol-acetonitrile-0.03 %phosphate solution(pH 2.85) in the proportion of 5:10:85(V/V/V) and(B) methanol-acetonitrile-0.03 %phosphate solution(pH 2.85) in the proportion of 5:10:85(V/V/V),using a linear gradient elution of 0 %B～100 %B at 0～18 minutes,then retaining for 5 minutes and returning to A.The flow rate was set at 0.5 mL/min and the ultraviolet detector was operated at 240 nm.Results Midazolam and internal standard were isolated on baseline in plasma apart from all other material.The the linear range was from 0.025～2.0 ?g/mL,and the detection limit was 2.5 ng/mL.The standard addition recoveries were from 99.98 %to 105.71 %and the extraction rates were from 91.29 %to 92.58 %.All of the intraday and interday variations were less then 14 %.The primary pharmacokinetics parameters of rat single dose intravascular administration were as follows:t1/2?was 0.582 h,Vd was 0.214 L,Cl was 0.584 L/h and AUC0→t was 0.419 ?g?h-1?mL-1.Conclusion Midazolam single dose intravascular administration has the characteristics of rapid distribution and elimination in blood and quick transport from blood to tissue.The method is sensitive,simple and suitable for the research of pharmacokinetie parameters of midazolam and description of possible pharmacological interactions of rat CYP3A1/2 or human CYP3A4/5 enzymes.

Objective To establish a HPLC fingerprint analysis method of salt-prepared Cortex Phellodendri. Methods C18 column was used, with gradient elution by the mobile phase consisted of acetonitrile-0.1 % phosphoric acid (contained 0.2 % triethylamine). The detection wavelength was at 230 nm, and the flow rate was at 0.8 mL/min. Results Seventeen characteristic peaks were selected and the fingerprints on HPLC was set up. Conclusion HPLC fingerprint method is reproducible, accurate and stable, and can be used for the quality control of salt-prepared Cortex Phellodendri.

Objective To determine the intestinal absorption characters of zingerone in rats.Methods The concentration of zingerone in the intestine perfusate was determined by HPLC.The absorption kinetics were obtained by the in-situ perfusion method in rats.Results The absorption rate constants(Ka) of zingerone were 0.0137,0.0135 and 0.0139 min-1 at the concentration of 25.0,50.0 and 100.0 ?g?mL-1,respectively;Ka of zingerone in the duodenum,jejunum,ileum and colon were 0.0070,0.0031,0.0032 and 0.0026 min-1,respectively.Conclusion Concentration of zingerone has no significant effect on its absorption kinetics.The best absorption segment of the intestine was duodenum,ileum,jejunum and colon by turns.The intestinal absorption of zingerone has the first-order kinetic characters with passive diffusion mechanism.

Objective To investigate the effect of borneol administered by gastric gavage or external application on ICAM-1 expression in rat retina with ischemia-reperfusion injury.Methods Rat ischemia-reperfusion model was established.Laser microscope and biological analysis microsoft were used to observe the change of ICAM-1 expression in retina or rats after gastric gavage or external application of borneol.Results Borneol administered by gastric gavage or external application obviously decreased the expression of ICAM-1 after the modeling for 24 hours,the difference being significant as compared with that in the model group(P

Objective To develop a HPLC-UV method for the determination of [6]-gingerol in the rat plasma.Methods [6]-Gingerol was extracted from the rat plasma by using liquid-liquid extraction,then was separated on a Hypersil  C18 column(250 mm?4.6 mm,5 ?m).The mobile phase was acetonitrile and water(45:55,v/v) with a flow-rate of 1.0 mL/min.The eluate from the HPLC column was monitored by the spectrophotometric detector at 280 nm.The injection volume was 20 ?L.[6]-Gingerol was identified based on its retention time compared with the reference standard.Results The linear calibration curve was obtained in the concentration range of 0.25～5.0 ?g/mL.The low quantitative limit was 0.1 ?g /mL.The RSD of inter-day was

Objective To study the interaction of tetramethylpyrazine and human serum albumin(HSA).Methods The fluorescent quenching of HSA with te tramethylpyrazine has been detecte d by fluorimetry.Results It was found that fluo-rescence of HSA were quenched by tetr amethylpyrazine.The fluorescent q uenching data were analyzed accordi ng to Stern -volmer equation and the bindi ng constant was obtained.Conclusion The mechanism of fluorescent quench ing is considered to be the formation of HSA-tetramethylpyrazine complex.

Objective: To separate and determine two constituents of Danshensu and protocatechuic aldehyde in compound Danshen Dropping Pill. Methods: The chromatographic conditions were as follow: Nucleosil C18 column, a mixture of methanol-water-acetic acid glacial (19∶80∶1) as the mobile phase with a flow rate of 1 mL per minute and the detection wavelength at 279 nm. P-hydroxybenoic acid was used as internal standard. Results: The calibration curves were linear in the range of 10～80 mg/L (r=0.9995) for Danshensu and 2～16 mg/L (r=0.9996) for protocatechuic aldehyde . The average recovery of Danshensu and protocatechuic aldehyde were 99.4 %and 99.5 %respectively. Conclusion: This method is accurate, simple and convenient, rapid and reproducible and can be used for the quality control of compound Danshen Pill.