The paper discussed inflammation related cells(including neutrophilic granulocyte, lymphocyte, monocytosis, macrophage, endothelial, vascular smooth muscle cells and glial cells), extracellular matrix even neurons and also their products(IL-1, IL-6, TNF-α, ICAM-1, ECM) interacted during ischemic stroke.

Positron emission tomography (PET) is one of the most advanced technology in the nuclear medicine currently. This paper reviewed the application of the PET in the acupuncture for clinical treatment in recent years.

Objective To construct the eukaryotic expression vector for IL-37 fused with the enhanced green fluorescent protein (EGFP) and express the fusion protein IL-37-EGFP in 293T cells. Methods The IL-37 and EGFP gene were amplified by PCR, respectively. Then the gene fragments of IL-37 and EGFP were in-serted into the pcDNA3.1 vector to construct the recombinant eukaryotic expression vector pcDNA3.1/IL-37-EGFP. The pcDNA3.1/IL-37-EGFP recombinant expression vector was identified by enzymatic digestion , DNA sequencing, PCR identification. Then the recombinant plasmid pcDNA3.1/IL-37-EGFP was used to transfect 293T cells , the expression of EGFP in 293T cells was detected by fluorescence microscope , the expression of IL-37 in 293T cells was detected by RT-PCR and Western blot. Results The results of enzymatic digestion and DNA sequencing showed that the fusion gene of IL-37 and EGFP linked with (G4S)3 was cloned correctly into pcDNA3.1 vector. The green fluorescence was observed in 293T cells transfected with pcDNA3.1/IL-37-EGFP by fluorescence microscope. RT-PCR and Western blot showed that the expression with high level of IL-37 mRNA and IL-37 protein in 293T cells transfected with pcDNA3.1/IL-37-EGFP , respectively. Conclusion The recom-binant eukaryotic expression vector for IL-37 fused with EGFP, pcDNA3.1/IL-37-EGFP, was constructed suc-cessfully and expressed effectively in 293T cells.

Professional features,which include taking students as its main body and improving the students' practical and operational ability as its aim are embodied in microbiology experimental teaching to improve the teaching quality and train high medical talents with different majors.Meanwhile,the design is intended to make medical graduates with different majors to quickly combine the microbiology knowledge with the practical situation in work thus to better serve the society.

Objective To evaluate the validity and responsiveness of the Chinese version of the miniBalance Evaluation Systems Test(mini-BESTest).Methods A total of 208 stroke patients were evaluated by 2 professionals using both mini-BESTest and Berg balance scale (BBS).All data were analyzed using Pearson correlation,t-test and descriptive statistical analysis.Results Significant correlation was observed between each entry of the mini-BESTest and its domain,with correlation coefficients ranging from 0.78 to 0.93 (P ＜ 0.05).The scores of whole assessment and the three related domains were significantly correlated between mini-BESTest and BBS results,the correlation coefficients ranging from 0.68 to 0.87 (P ＜ 0.05).After 2 weeks of treatment,there was significant improvement in each domain and in the total assessment score (P ＜ 0.05).No floor or ceiling effects were observed in the total mini-BESTest scores,but there was a slight ceiling effect in the anticipatory and sensory orientation domains,and a slight floor effect in the reactive postural control domain.Conclusion The mini-BESTest has good validity and responsiveness,and can be applied to evaluate patients after stroke.

Objective To study the effects of mindfulness behavior training on coping style and illness uncertainty in patients of shoulder hand syndrome (SHS) after ischemic stroke.Methods 65 cases with SHS were randomly divided into two groups by digit method: the control group (n=32) and experimental group (n=33).Patients in control group only received routine rehabilitation, while patients in experimental group also received mindfulness behavior training.The daily life, medical coping style, illness uncertainty and mindfulness were evaluated respectively by Barthel Index (BI) , Medical Coping Style Questionnaire (MCMQ) , Illness Uncertainty Scale (IUS) and Mindful Attention Awareness Scale (MAAS).Results Before training, there were no significant difference in BI,MCMQ,IUS and MAAS (P＞0.05).After treatment, scores in BI improved significantly in the experimental group than that in the control group(72.4± 11.6 vs 62.9±10.1) ,scores in IUS improved significantly in the experimental group than that in the control group(69.3±9.3 vs 86.9±7.2) and scores in MCMQ and MAAS improved significantly in the experimental group than that in the control group(P＜0.05).26 cases whose Barthel index was more than 60 points in experimental group after treatment were found while 17 cases whose Barthel index was more than 60 points in control group were done after treatment (x2 =6.415, P＜0.05).Conclusion Mindfulness behavior training can regulate the patient coping style and weaken illness uncertainty,and improve functions rehabilitation.

Objective To explore the application of ultrasound- guide modified Seldinger technique for upper arm PICC insertion in infant patients. Methods Use the ultrasound-guide modified Seldinger technique to inserted PICC for 27 infant patients. Results All 27 cases were inserted successfully, success rate of the one puncture reached 92.6%. Conclusions By adequate preparation for infants, effective immobilization and good cooperation of operators, and combined with techniques which prevent PICC misplacement, overcome the shortcoming of bad vessel and non-compliance of infant patients, develop the advantage of ultrasound, could improve the rate of successful catheterization of upper arm PICC insertion in infant patients, and protect the vessels.

目的探讨以问题为基础的教学法(PBL)和康复病例实验报告两种教学法在运动疗法(PT)模块中的应用效果。方法选择2008 级四年制康复治疗学专业本科学生88 名，1 班采用PBL教学模式，2 班采用理论、实践相结合的康复病例采集与书写模式，采用自制教学效果评价表、学生实习跟踪调查进行评价。结果两班学生综合素质和分析问题的能力均有提高。2 班在观察能力、动手能力、理论与实践结合、疾病信息采集、分析判断能力、临床适应能力、服务态度与同情心、康复评定的全面性等方面好于1 班(P<0.05)，1 班在信息技术利用方面优于2 班(P<0.05)。结论融基础知识、专业知识、临床实践于一体，以书写康复病例为学科综合，培养学生以功能障碍为纲的临床思维，可以提高学生的动手能力、分析判断能力、医患沟通能力和临床适应能力，做到了课堂-见习-实习密切连接。

Objective To investigate the effect of somatic sense interactive game Kinect on executive dysfunction for patients with stroke. Methods 78 inpatients with first stroke were divided into intervention group and control group, and 35 cases in the intervention group and 36 cases in the control group finished the study. The control group accepted conventional rehabilitation, and the intervention group played the game of Sailing for Gold Coins and Golf of the Kinect in addition. They were assessed with Behavioral Assessment of Dysexecutive Syndrome (BADS) before and 4 weeks after intervention. Results The difference of scores of all the sub-scales and total of BADS before and after intervention were more in the intervention group than in the control group (P<0.05). Conclusion The somatic sense interactive game Kinect can be used to improve the executive function of the patients with stroke.

Objective To research the effects of somatic sense interactive game Kinect on balance and walking function in stroke patients.Methods 40 patients with stroke were divided into control group (n=20) and experimental group (n=20), who accepted conventional rehabilitation and additional somatic sense interactive game Kinect. They were evaluated with gait analysis, Berg Balance Scale, Holden Walking Function Rating Scale and Barthel Index before and after treatment. Results Both groups improved in all the indices after 4 weeks of treatment (P<0.001), and improved more in the experimental group than in the control group (P<0.05). Conclusion The somatic sense interactive game Kinect could further improve the balance and walking function of stroke patients.