0.05). But after 12 weeks, blood glucose level and urine volume in DM group were higher and greater than those in control group (both P0.05). (2) The urine osmolality in DM group was decreased while the plasma osmolality increased (P

Objective To study the relation between the regulation of mannose‐binding lectin (MBL) level and pulmonary tuber‐culosis(TB) susceptibility .Methods A total of 142 inpatients with pulmonary TB and 120 healthy controls were recruited to par‐ticipate in this case‐control study .Serum MBL level was detected ,meanwhile the restriction fragment length polymorphism (RFLP) was adopted to detect MBL2 gene polymorphism .Results The one‐way analysis of variance was adopted to analyze the MBL level in different genotype groups ,including the group YA/YA ,XA/YA ,XA/XA ,YA/YB ,XA/YB and YB/YB ,it was found that the MBL level had statistical differences among 3 groups and between any two groups(P<0 .01) .The MBL level in the pulmonary TB group was significantly higher than that in the healthy control group (P< 0 .01) .Among the healthy controls ,the MBL level in 93 .2% individuals(55/59) carrying YA/YA genotype was>1 000 ng/mL ,while which in 100% individuals(26/26) carrying geno‐type XA/XA or allele B was≤1 000 ng/mL .Conclusion The MBL level may be associated with the susceptibility to pulmonary TB .The YA/YA gene for determining high MBL level may be a protected gene .

Objective: To design a heart rate testing procedure which based on Labview. Methods:Collect the blood oxygen signals of the fingers, which keep in step with the pulse wave. Filter the signals and obtain the pregnant datum by the Labview procedures. Results: Achieve the pulse cycle“T”by Labview processing the blood oxygen signals of the fingers, and gain the heart rate by calculating the pulse in one minute(Pulse is equal to the number of heart beat in one minutes-1/T,the heart rate). Conclusion:It is an important and meaningful work to process the signals. Not only acquire the heart rate, but also prompt and diagnose the diseases of the cardiovascular system. It also can provide some meaningful diagnostic evidence.

OBJECTIVE:To observe the clinical efficacy and safety of Qingjin huatan decoction for patients with AECOPD phlegm-heat obstructing lung. METHODS:120 patients with AECOPD phlegm-heat obstructing lung were divided into observation group and control group according to random number table,with 60 cases in each group. Control group received routine therapy as low-flow oxygen therapy,anti-infective,bronchiectasis,expectorant,etc.;observation group additionally received Qingjin huatan decoction(decocted by 400 ml pure water),tid,on the basis of control group. The levels of IL-8,IL-17,IL-23 and TNF-α were observed in 2 groups before and 7 d after treatment. Clinical efficacies and the occurrence of ADR were compared between 2 groups. RESULTS:Before treatment,there was no statistical significance in the levels of IL-8,IL-17,IL-23 and TNF-α between 2 groups (P>0.05). After treatment,the levels of IL-8,IL-17,IL-23 and TNF-α in observation group were significantly decreased and lower than in control group,with statistical significance (P<0.05). The levels of IL-17,IL-23 and TNF-α in control group were decreased significantly compared to before treatment,with statistical significance(P<0.05);the level of IL-8 had no signifi-cant change compared to before treatment,without statistical significance (P>0.05). Excellent rate of observation group was 95.00%,which was significantly higher than 78.95% of control group,with statistical significance(P<0.05). There was no statis-tical significance in the incidence of ADR between 2 groups(P>0.05). CONCLUSIONS:Qingjin huatan decoction shows signifi-cant therapeutic efficacy in the treatment of AECOPD phlegm-heat obstructing lung,and can improve inflammatory factors with good safety.

0.05).Conclusion The polymorphism of rs2285676 in KCNJ11 may not be associated with T2DM in Hanethnic population in Chongqing.

Objective To analyze and evaluate the 48 selected highly polymorphic SNP loci in Chinese Han population on genetic and forensic aspects.Methods Samples from 200 unrelated Han individuals in East China were collected.48 X-SNP polymorphic genetic markers were selected according to the information providedby NCBI and HapMap,and then were typed by SNPlex~(TM) System for establishing genetic data.Results The 48 X-SNP loci were highly polymorphic markers in East China Han population except rs6527549,and their polymorphism information contents(PIC)were all above 0.32,the discrimination power(DP)in females and males were above 0.56 and 0.40 respectively,the probability of exclusion(PE)in duos and trios were above 0.20 and 0.32 respectively.In addition,linkage disequilibriums were observed among some loci.Conclusion The 48 X-SNP loci union is suitable for developing high-throughout automated research and also useful for special parentage testing.

Objective To investigate the effects of chloroquine on metabolism of insulin,glucose,lipids and expression of insulinase gene (EIG) in patients with type 2 diabetes mellitus.Methods Fasting plasma insulin (FINS),fasting plasma glucose (FPG),glycoslated hemoglobin (HbA1c),plasma lipids and lipoprotein,insulinase activity of erythrocytes (EIA) and EIG were determined in 30 patients with type 2 diabetes mellitus laking chloroquine for 14 day in dosage of 250mg twice daily,27 diabetic patients laking placebo and 20 normal subjects.Insulin sensitive index (ISI) were also calculated.Results Chloroquine caused a decrease in total plasma cholesterol,low density lipoprotein,EIA and EIG,and an increase in ISI,plasma high density lipoprotein and subclass 2.No change of these indices was observed in diabetic patients with placebo.Conclusion Chloroquine can ameliorate the dyslipidemia and insulin sensitivity of patients with type 2 diabetes mellitus.This may be due to a decrease in EIG,which may result in lowered degradation of insulin.

Objective To study plasma tumor necrosis factor ?(TNF ?) level and its relationship with insulin resistance in familial type 2 DM. Methods Plasma TNF ? were determined by RIA in 32 familial type 2 DM patients, their 37 non diabetic first degree relatives and 40 healthy control subjects. Results Plasma TNF ? was significantly higher in familial type 2 DM patients[(1.19?0.21)?g/L] than in healthy control subjects[(1.00?0.18)?g/L]( P 0.05). Regression analysis indicated that TNF ? was inversely related to ISI and was a significantly independent contributor to variations in ISI. 39% of the variance in ISI was explained by TC, body mass index(BMI)and TNF ?. Conclusion The finding of an association between high plasma levels of TNF ? and insulin resistance suggests that TNF ? may be involved in the pathophysiology of insulin resistance in familial type 2 DM patients.

BACKGROUND:Because of poor biocompatibility, conventional intraocular lenses (IOLs) used to improve visual acuity can result in a higher incidence of inflammatory foreign body reactions.
OBJECTIVE:To study the biocompatibility and therapeutic effects of hirudin peptide-modified acrylic IOL on eye diseases.
METHODS:By immersion coating method, we prepared a polymethylmethacrylate IOL by the modification with hirudin polypeptides and observed its biocompatibility through the cytological observation. In this study, six New Zealand white rabbits were equivalently randomized into conventional and hirudin peptide grouppolypeptide hirudin group. Rabbits in the conventional group were given conventional lens implantation; while those in the hirudin peptide group underwent hirudin peptide-modified polymethylmethacrylate IOL.
RESULTS AND CONCLUSION:Three days after implantation of hirudin peptide-modified polymethylmethacrylate IOL, cels proliferated and spread wel on the acrylic IOL, but on the cel membrane, there were a few cels most of which were gathered sphericaly with no spreading. In the hirudin peptide group, some cels attached to the border zone migrated to the IOL surface, but did not impede the central visual field; in the conventional group, the IOL surface was stil covered by fibrotic cel membrane.Comparedwith the conventional group, the score in the SchirmerItest was increased but the breakup time of tear film decreased in the hirudin peptide group. To conclude, the polymethylmethacrylate IOL modified by hirudin peptides has good physicochemical properties and biocompatibility, increases visual acuity, and yields satisfactory outcomes in the cataract treatment, which provides a new insight into the treatment of cataract.

T) in PRKAA2 is not associated with T2DM in Han population in Chongqing area.