1.Morphological characterization of Haemonchus contortus in goats (Capra hircus) and sheep (Ovis aries) in Penang, Malaysia.
Wahab A Rahman ; Suhaila Abd Hamid
Tropical biomedicine 2007;24(1):23-7
The large stomach worm, Haemonchus contortus is an important pathogen of goats (Capra hircus) and sheep (Ovis aries). This paper describes characteristics of surface cuticular ridges (synlophe) of H. contortus adults from the two hosts. There were more ridges in H. contortus from goats compared to that from sheep. Total body length, vulvar morphology, spicule length and cervical papillae had been considered as markers of physical adaptation and were studied and described.
Sheep
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Haemonchus contortus
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Length
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upper case aitch
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Malaysia
2.Separation of sulfated urinary glycosaminoglycans by highresolution electrophoresis for isotyping of mucopolysaccharidoses in Malaysia
Nor Azimah Azize ; Zabedah Md Yunus ; Norsiah Md Desa ; Ngu Lock Hock ; Suhaila Abd Rahman
The Malaysian Journal of Pathology 2010;32(1):35-42
Mucopolysaccharidoses (MPS) are a group of inherited disorders caused by the defi ciency of specifi c
lysosomal enzymes involved in glycosaminoglycans (GAGs) degradation. Currently, there are 11
enzyme defi ciencies resulting in seven distinct MPS clinical syndromes and their subtypes. Different
MPS syndromes cannot be clearly distinguished clinically due to overlapping signs and symptoms.
Measurement of GAGs content in urine and separation of GAGs using high-resolution electrophoresis
(HRE) are very useful initial screening tests for isotyping of MPS before specifi c enzyme diagnostics.
In this study, we measured total urinary GAGs by a method using dimethylmethylene blue (DMB),
and followed by isolation and separation of GAGs using high resolution electrophoresis (HRE)
technique. Of 760 urine samples analyzed, 40 have abnormal GAGs HRE patterns. Thirty-fi ve
of these 40 cases have elevated urinary GAGs levels as well. These abnormal HRE patterns could
be classifi ed into 4 patterns: Pattern A (elevated DS and HS; suggestive of MPS I, II or VII; 16
cases), Pattern B (elevated HS and CS; suggestive of MPS III; 17 cases), and Pattern C (elevated
KS and CS; suggestive of MPS IV, 5 cases), and Pattern D (elevated DS; suggestive of MPS VI;
2 cases). Based on the GAGs HRE pattern and a few discriminating clinical signs, we performed
selective enzymatic investigation in 16 cases. In all except one case with MPS VII, the enzymatic
diagnosis correlated well with the provisional MPS type as suggested by the abnormal HRE pattern.
Our results showed that GAGs HRE is a useful, inexpensive and practical fi rst-line screening test
when MPS is suspected clinically, and it provides an important guide to further enzymatic studies
on a selective basis.