This paper emphasizes constitution factor,the chief pathogenesis of repeated respiratory tract infection(RRI) of children;the main pathogenesis are Qi deficiency of spleen-lung and retention of phlegm.According to children's physiology feature,spleen is frequently insufficient and RRI Children are given too much antibiotic and bitter-cold herbs,that causes damage to stomach.Regulating spleen is expected to figure prominently in prevention and treatment of RRI children.Regulating spleen not only means reinforcing the spleen,but also promoting digestion to eliminate stagnation and regulating Qi and eliminating phlegm to restore the normal physiological function of spleen and stomach,strengthen children's constitution and immunity.There are 4 therapeutic principles of RRI children: activating spleen,promoting lung and resolving phlegm during the acute phase;reinforcing the spleen,eliminating stagnation and eliminating phlegm;reinforcing the spleen and promoting lung during the deferment period;reinforcing the spleen and enriching Qi for prevention.

0.05).Conclusion SjvO_2 and OEF may objectively reflect brain oxygen metabolism and cerebral blood flow after acute cerebral infarction. Furthermore, they are valuable indicators for predicting prognosis.

Objective In order to control the organic pollution in surface water sources in Jiaxing area, deep water treatment method was applied to treat drinking water. The effectiveness and correlative hygiene issues of the constructive project were pre,assessed. Methods On the basis of selecting and comparing some water factories in domestic and abroad which were similar with this constructive project, the assessment was synthetically carried out by surveying on the spot, checking blueprint, examining technological process and equipments layout, worked technique and materials, after experimental deep water,treatment was pup in to pilot scale test. Results The drinking water deep treatment method could eliminate organic substances effectively from original water. The results of the pilot test showed that the removed rates of CODMn, TOC, color degree and NH3,N through this measure were respectively 95.57%, 25.35%, 32.80% and 37.40% higher than those through route drinking water treatment measures. The measure could translate Ames test result into negative. The dosage of alum and chlorine could be reduced 33% and 50% respectively. Water purity and taste could be improved. The arrangement and layout of the facilities of this constructive project was advanced and reasonable, which was conformed to the hygienic requirements. Conclusion This constructive project could effectively solve the serious pollution problem of organic substances in water source in Jiaxing area and improve the purification effectiveness of drinking water and water quality. The occupational hazards that possibly present in the productive process of this project could be prevented. From the view of hygiene, this constructive project was feasible.

Objective To investigate the clinical characteristics and diagnosis of some patients with cryptococcus neoformans meningitis(CNM).Methods The clinical features were analyzed and summarized for the findings of 16 CNM cases.Results The clinical cachexia manifestations were not found in the 16 CNM cases.The higher intracranial hypertension and the slight elevation of CSF protein content were found in the earlier period.The cryptococcus neoformans could be found in CSF through lots of lumbar puncture usually.Conclusion The symptom in the earlier period of cryptococcus neoformans meningitis is not typical.It is difficult to find the cryptococcus neoformans.In order to definite the diagnosis,more examinations of CSF must be done.

[Objective] To develop an HPLC method for determining Gallic acid in Milin Capsules.[Method] The contents of Gallic acid were determined by HPLC.Agilent1200 HPLC.Chromatography column was ZORBAXSB-C18(4.6?250mm,5?m);mobile phase:methanol-0.2% phosphoric(6∶94);flow rate was 1.0ml/min;UV detection wave length was 270nm.[Results] The average recovery of Gallic acid was 99.10%,RSD was 1.07%.[Conclusion] This method was simple,quick,stable,accurate,can be used as a method for determining Gallic acid in Milin Capsules.

Objective To apply fluo-rescencc-activated cell sorting(FACS) in sorting T lymphocyte (CD_3~+) and granulocyte (CD_(15)~+), which establish the separating method of series of cells from human peripheral blood, so that the scientific research and clinical research could be carried out were specifically. Methods 10 cases of normal peripheral blood were collected and T lymphocyte (CD_3~+) and granulocyte (CD_(15)~+) were stained with florescence conjugated antibodies. The positive cells were sorted by FACS. Results Before sorting the peripheral blood, the proportion of the T lymphocyte(CD_3~+) and granulocyte (CD_(15)~+) in the leukocyte is 48.8 % and 30.8 %; after sorting by FACS, the purity of T lymphocytes (CD_3~+) is up to 98 % and the recovery is about 95 %; the purity of granulocyte (CD_(15)~+) is up to 97 % and the recovery is about 96 %. Conclusion The FACS could Mlow us to quickly sort T lymphocyte (CD_3~+) and granulocyte (CD_(15)~+) with higher recovery and higher purity from the peripheral blood.

Astrocytoma ; metabolism ; pathology ; Biomarkers, Tumor ; metabolism ; Brain Neoplasms ; metabolism ; pathology ; Cell Proliferation ; Diagnosis, Differential ; Glioblastoma ; metabolism ; pathology ; Humans ; Ki-67 Antigen ; metabolism ; Melanoma ; metabolism ; pathology ; Meningeal Neoplasms ; metabolism ; pathology ; Meningioma ; metabolism ; pathology ; Nevus ; metabolism ; pathology ; Proliferating Cell Nuclear Antigen ; metabolism ; Skin Neoplasms ; metabolism ; pathology

Objective To investigate the expressions and clinical significance of survivin, and lymphatic vessel endothelial hyaluronan receptor 1 ( LYVE-1) in gastric cancers.Methods The expres-sions of survivin and LYVE-1 were detected with streptavidin avidin-peroxidase ( SP) immunohistochemical method in 70 cases of gastric cancers and 50 cases of normal gastric tissues.The relationship between the expressions of survivin and LYVE-1 with the clinicopathological parameters was analyzed.Results The ex-pression rate of survivin was 65.71%(46/70) in gastric cancers, and 6.00% (3/50) in normal gastric tissues, with statistically significant difference between two groups (χ2 =43.048, P <0.01) .The expres-sion of survivin was different in gastric cancers:survivin expression rate was 76.92%in poorly differentia-ted gastric carcinomas, 75.00%in T3 ~T4 gastric carcinomas, 75.61%in the positive lymph node metas-tasis of gastric cancers, and 77.08%in the Ⅲ~Ⅳ gastric cancer tissues; which was significantly higher than the highly differentiated (51.61%), T1 ~T2(38.89%), lymph node-negative (51.72%), andⅠ~Ⅱperiod (40.91%) ( P <0.05).The expression of survivin in gastric cancers was no obvious correlation with the age, gender and tumor site ( P >0.05).The expression rate of LYVE-1 was 32.86%(23/70) in gastric cancers, and 4.00%(2/50) in gastric ulcers, with significant difference between two groups (χ2 =14.726, P <0.01) .The expression of LYVE-1 was different in gastric cancers:LYVE-1 expression rate was 40.38%in T3 ~T4 gastric carcinomas, 46.34%in the positive lymph node metastasis of gastric canc-ers, 41.67% in the Ⅲ ~Ⅳ gastric cancer tissues; which was significantly higher than the T1 ~T2 (11.11%), lymph node-negative (13.79%), andⅠ~Ⅱperiod (13.64%) ( P <0.05).The expres-sion of LYVE-1 in gastric cancers was no obvious correlation with the age, gender, tumor site, and the de-gree of differentiation ( P >0.05 ) .There was a positive correlation between expressions of survivin and LYVE-1 in gastric cancers ( r =0.271, P <0.05).Conclusions Survivin and LYVE-1 were abnormally expressed in gastric cancers with a colose relationships between two parameters.They might play important roles in tumorigenesis and progression of a gastric cancer.

Objective:To compare the effect of four different techniques on removal of vapor lock in the apical region of curved root canals.Methods:Forty simulated resin root canals with 45° curvature were prepared using WaveOne Primary,then the apical foramen were sealed with soft wax.The teeth were divided randomly into 4 groups thereafter (n =10).Contract solution was injected into the canals using a 30 G side-vented needle and scanned with cone-beam CT (CBCT) to identify the volume of the vapor lock.Four different techniques including photon-induced photoacoustic streaming (PIPS) laser-activated irrigation,gutta-percha cone technique,ultrasonic irrigation,and sonic irrigation were used to remove the vapor locks in the root canals.The residual volume of the vapor lock was identified again using CBCT scanning data.Accordingly,the reduction rates of the vapor lock were calculated.Furthermore,the initial and residual vapor lock length was calculated.The data were analyzed by using the One-Way ANOVA analysis and Kruskal-Wallis H test at a significance level of P ＜ 0.05.Results:There was no significant difference in the initial vapor lock volume (P ＞ 0.05).Residual volume of the vapor lock for PIPS laser-activated irrigation was 0 mm3,and that for gutta-percha cone technique was (0.02 ± 0.07) mm3,significantly lower than those of ultrasonic and sonic irrigation,the values being (0.20 ± 0.09)mm3 and (0.23 ±0.06) mm3 (P ＜0.001),respectively.The reduction rates of the vapor lock of PIPS laser-activated irrigation and gutta-percha cone technique were 100.00％ (100.00％,100.00％) and 100.00％ (77.66％,100.00％),respectively,significantly higher than those of ultrasonic irrigation [70.37％ (56.41％,91.43％)] and sonic irrigation [63.54％ (51.47％,74.00％),P ＜0.001].The length of the residual vapor lock for PIPS laser-activated irrigation was 0 mm,and that for gutta-percha cone technique was (0.15 ±0.47) mm,significantly lower than those of ultrasonic and sonic irrigation,values being (2.21 ±0.09) mm and (2.34 ±0.08) mm (P ＜0.001),respectively.The length of the residual vapor locks in the ultrasonic and sonic group remained approximately the same as the distance between the working tip and the apical foramen.Conclusion:PIPS laser activated irrigation and gutta-percha cone technique could remove the vapor lock from the apical region of curved canals effectivelv.

0.05). Conclusion: There is surface potential in teeth and the potent ial can be affected by the electrolyte surroundings.