This paper emphasizes constitution factor,the chief pathogenesis of repeated respiratory tract infection(RRI) of children;the main pathogenesis are Qi deficiency of spleen-lung and retention of phlegm.According to children's physiology feature,spleen is frequently insufficient and RRI Children are given too much antibiotic and bitter-cold herbs,that causes damage to stomach.Regulating spleen is expected to figure prominently in prevention and treatment of RRI children.Regulating spleen not only means reinforcing the spleen,but also promoting digestion to eliminate stagnation and regulating Qi and eliminating phlegm to restore the normal physiological function of spleen and stomach,strengthen children's constitution and immunity.There are 4 therapeutic principles of RRI children: activating spleen,promoting lung and resolving phlegm during the acute phase;reinforcing the spleen,eliminating stagnation and eliminating phlegm;reinforcing the spleen and promoting lung during the deferment period;reinforcing the spleen and enriching Qi for prevention.

0.05).Conclusion SjvO_2 and OEF may objectively reflect brain oxygen metabolism and cerebral blood flow after acute cerebral infarction. Furthermore, they are valuable indicators for predicting prognosis.

[Objective] To develop an HPLC method for determining Gallic acid in Milin Capsules.[Method] The contents of Gallic acid were determined by HPLC.Agilent1200 HPLC.Chromatography column was ZORBAXSB-C18(4.6?250mm,5?m);mobile phase:methanol-0.2% phosphoric(6∶94);flow rate was 1.0ml/min;UV detection wave length was 270nm.[Results] The average recovery of Gallic acid was 99.10%,RSD was 1.07%.[Conclusion] This method was simple,quick,stable,accurate,can be used as a method for determining Gallic acid in Milin Capsules.

Objective In order to control the organic pollution in surface water sources in Jiaxing area, deep water treatment method was applied to treat drinking water. The effectiveness and correlative hygiene issues of the constructive project were pre,assessed. Methods On the basis of selecting and comparing some water factories in domestic and abroad which were similar with this constructive project, the assessment was synthetically carried out by surveying on the spot, checking blueprint, examining technological process and equipments layout, worked technique and materials, after experimental deep water,treatment was pup in to pilot scale test. Results The drinking water deep treatment method could eliminate organic substances effectively from original water. The results of the pilot test showed that the removed rates of CODMn, TOC, color degree and NH3,N through this measure were respectively 95.57%, 25.35%, 32.80% and 37.40% higher than those through route drinking water treatment measures. The measure could translate Ames test result into negative. The dosage of alum and chlorine could be reduced 33% and 50% respectively. Water purity and taste could be improved. The arrangement and layout of the facilities of this constructive project was advanced and reasonable, which was conformed to the hygienic requirements. Conclusion This constructive project could effectively solve the serious pollution problem of organic substances in water source in Jiaxing area and improve the purification effectiveness of drinking water and water quality. The occupational hazards that possibly present in the productive process of this project could be prevented. From the view of hygiene, this constructive project was feasible.

Objective To investigate the clinical characteristics and diagnosis of some patients with cryptococcus neoformans meningitis(CNM).Methods The clinical features were analyzed and summarized for the findings of 16 CNM cases.Results The clinical cachexia manifestations were not found in the 16 CNM cases.The higher intracranial hypertension and the slight elevation of CSF protein content were found in the earlier period.The cryptococcus neoformans could be found in CSF through lots of lumbar puncture usually.Conclusion The symptom in the earlier period of cryptococcus neoformans meningitis is not typical.It is difficult to find the cryptococcus neoformans.In order to definite the diagnosis,more examinations of CSF must be done.

Objective To apply fluo-rescencc-activated cell sorting(FACS) in sorting T lymphocyte (CD_3~+) and granulocyte (CD_(15)~+), which establish the separating method of series of cells from human peripheral blood, so that the scientific research and clinical research could be carried out were specifically. Methods 10 cases of normal peripheral blood were collected and T lymphocyte (CD_3~+) and granulocyte (CD_(15)~+) were stained with florescence conjugated antibodies. The positive cells were sorted by FACS. Results Before sorting the peripheral blood, the proportion of the T lymphocyte(CD_3~+) and granulocyte (CD_(15)~+) in the leukocyte is 48.8 % and 30.8 %; after sorting by FACS, the purity of T lymphocytes (CD_3~+) is up to 98 % and the recovery is about 95 %; the purity of granulocyte (CD_(15)~+) is up to 97 % and the recovery is about 96 %. Conclusion The FACS could Mlow us to quickly sort T lymphocyte (CD_3~+) and granulocyte (CD_(15)~+) with higher recovery and higher purity from the peripheral blood.

Objective To investigate the effect of penehyclidine (PHCD) on Toll-like receptor 4 (TLR4)mRNA and Toll-like receptor 2 (TLR2) mRNA expression in the lung tissue in rats with acute lung injury induced by lipopolysaccharide (LPS) .Methods Sixty healthy SD rats of both sexes weighing 200-220 g were randomly divided into 5 groups ( n = 12 each) :control group (group C) , LPS group and P1-3 groups. Acute lung injury was induced by intraperitoneal (IP) LPS 8 mg/kg in LPS and P1-3 groups. PHCD 0.3, 1.0 and 3.0 mg/kg were given IP after LPS administration in P1-3 groups. The animals were anesthetized at 6 h after IP LPS. Blood samples were collected for determination of serum TNF-α and IL-6 concentrations ( by ELISA) and then sacrificed, the lungs were immediately removed for determination of TLR4 mRNA and TLR2 mRNA expression (by RT-PCR), and microscopic examination. Results LPS significantly increased TLR4 mRNA and TLR2 mRNA expression in the lung tissue and serum TNF-α and IL-6 concentrations. PHCD 1.0 or 3.0 mg/kg significantly inhibited LPS-induced increase in TLR4 mRNA and TLR2 mRNA expression in the lung tissue and serum TNF-α and ILr6 concentrations.The lung histopathologic damage was significantly ameliorated in P2 and P3 groups as compared with group LPS.Conclusion PHCD can protect the lungs against LPS-induced acute lung injury through inhibiting TLR4 mRNA and TLR2 mRNA expression in the lung tissue and reducing the inflammatory response.

Objective To observe any changes in spinal cord hemodynamics after spinal cord injury and to investigate their mechanism. Methods Twenty female Sprague-Dawley rats were divided into a control group and a moderate injury group,10 rats in each group.A rat model of spinal cord injury caused by moderate compression was established using Nystrom's method.Changes in the velocity and volume of blood flow and in the diameters of vessels of the spinal cord were monitored for 6 hours using laser speckle imaging. Results No changes in the velocity or volume of blood flow or in the diameter of vessels were observed in the control group,but all of these indices diminished significantly in the injury group. Conclusions Laser speckle imaging can be used to monitor changes in spinal cord hemodynamics.Such changes after acute spinal cord injury indicate progressive post-injury ischemia.The occlusion of veins may be an important contributor to post-injury ischemia.

Objective To discuss effect of intellectual training on cognitive function, daily life ac-tivity and life quality of patients with Alzheimer's disease. Methods We used clinical dementia rating (CDR) scale to selecf 38 patients with mild to median degree of Alzheimer's disease.Intellectual training was carried out for 1 year and at the same time their family members took part in it and dietary instruction was given. Mini-mental state examination (MMSE), information-memory-concentration test (IMCT) and activity of daily living scale (ADL) were used as evaluation tools before and after training. Results The patients' degree of defect before training was not different from that of within the first three months after training (P＞0.05),but greatly alleviated 6 months and one year later (P＜ 0.05,P＜ 0.01 ). Conclusions Beneficial intellectual training could improve the cognitive function of patients with Alzheimer's disease.It alleviated their symptom,prolonged the process of dementia and increase the living and life quality of them.

Astrocytoma ; metabolism ; pathology ; Biomarkers, Tumor ; metabolism ; Brain Neoplasms ; metabolism ; pathology ; Cell Proliferation ; Diagnosis, Differential ; Glioblastoma ; metabolism ; pathology ; Humans ; Ki-67 Antigen ; metabolism ; Melanoma ; metabolism ; pathology ; Meningeal Neoplasms ; metabolism ; pathology ; Meningioma ; metabolism ; pathology ; Nevus ; metabolism ; pathology ; Proliferating Cell Nuclear Antigen ; metabolism ; Skin Neoplasms ; metabolism ; pathology