Humans ; Metabolic Syndrome

Objective:To investigate the mechanism of Smad7 gene modified bone marrow mesenchymal stem cells ( Smad7-BMSCs) to prevent hepatic fibrosis in vitro. Methods:Smad7-EGFP-BMSCs were established by isolating and purifying BMSCs of rats, and transfecting Ad-Smad7-EGFP. HSC-T6 were divided into Group A, Group B, Group C and Group D, which were respectively incubated with Smad7-EGFP-BMSCs, BMSCs, Smad7 plasmid and PBS for 72 hours. The level of Smad7and TGF-β1protein in the culture solution was determined by ELISA. The expression of mRNA and protein of Smad7,TGF-β1,Col Ⅰ and α-SMA in the hepatic stellate cells were respectively determined by Western blot and RT-PCR. Cellular apoptosis was determined by flow cytometry. Results:(1)The results of ELISA showed that the level of TGF-β1 protein decreased(P<0. 01) but the level of Smad7 protein increased (P<0. 01) in Group B,Group C and Group D compared with Group A;the level of TGF-β1 protein decreased(P<0. 01) but the level of Smad7 protein increased (P<0. 01) in Group D compared with Group B and Group C. (2)The results of Western blot and RT-PCR showed that the level of mRNA and protein of Smad7,TGF-β1,Col Ⅰ and α-SMA decreased(P<0. 01) but the level of mRNA and protein of Smad7 protein increased (P<0. 01) in Group B,Group C and Group D compared with Group A;the level of mRNA and protein of Smad7,TGF-β1,ColⅠandα-SMA decreased(P<0. 01) but the level of mRNA and protein of Smad7 protein increased (P<0. 01) in Group D compared with Group B and Group C. (3)The results of flow cytometry showed that the rate of cellular apoptosis de-creased(P<0. 01),but the level of Smad7 protein increased (P<0. 01) in Group B,Group C and Group D compared with Group A;the rate of cellular apoptosis decreased(P<0. 01)in Group D compared with Group B and Group C. Conclusion:Smad7-BMSCs can have the effect of anti-hepatic fibrosis by affecting TGF-β1 signal pathway and promoting cellular apoptosis in hepatic stellate cells.

The clinical manifestations of neonatal shock are atypical so that the early identification was important.Heart rate variability analysis and pulse oximetry for perfusion index monitoring are great help for early identification of neonatal shock.Much attention should be given to the treatment of neonatal special type of shock in addition to follow the general principles of shock treatment.

Nanopublication is a novel semantic expression pattern.The relation between nanopublication and knowledge discovery was studied in terms of its emergence, improvement and progression in order to provide certain clues for discovering new knowledge.

[Objective]The purpose of the review was to summarize the research progress on traditional Chinese medicine to prevent and treat scar and its mechanism in recent 10 years. [Methods]Retrieving the literature of the past 10 years on Chinese medicine to prevent scar , it is to summarize scar mecha-nism of prevention and cure both in vivo and in vitro experiments. [Result]Main mechanism of preventing and treating scar by traditional Chinese medicine is through studying fibroblasts and col agen. The specific mechanism is that Chinese medicine can inhibit the growth of scar fibroblasts in a con-centration-time-dependent manner, so as to reduce the scar tissue and control of infection and other complications; Additionly, by adjusting the levels of col agenⅠ,Ⅲ, Chinese medicine can regulate synthesis of col agen, reduce scar, and improve the quality of wound repair. [Conclusion]Traditional Chinese medicine has certain advantages in preventing and treating scar, but needs to further standardize methodology to broaden ideas. So Chinese medicine has a promising furure on anti-scar in the clinic.

Objective To discuss the nursing intervention effect on reducing anxiety of patients with amputation due to trauma. Methods 30 trauma patients needing amputation were given pertinent nursing intervention according to the psychological problems existing in them by special nurses from admission to operation.Self-rating anxiety scale (SAS)was used to evaluated the anxiety of patients ten minutes after informing them of the operation and ten minutes before the operation.The anxiety level was compared before and after the intervention. Results After the nursing intervention,the level of anxiety was alleviated compared with that on admission. Conclusions Strengthening the nursing intervention can effectively reduce the anxiety level of patients with amputation due to trauma before operation,and improve the initiative and compliance of treatment in patients.

Objective To explore the curative effect of loop electrosurgical excision procedure (LEEP) on cervical intra-epithelial neoplasia(CIN) infected with high risk human papilloma virus (HR-HPV).Methods 80 CIN patients with HPV infection underwent LEEP were selected.HR-HPV DNA load was detected before operation and 3 months after operation,and at the same time,vaginal examination and cervical directed biopsy were conducted.Results Before LEEP operation,the differences between different levels of CIN HPV load were not significant(P ＞0.05,F =0.45).3 months after operation,CIN Ⅰ grade 19 cases (86.4％) turned to chronic inflammation,CIN Ⅱgrade 36 cases (83.7％) turned to chronic inflammation,CINⅢ grade 13 patients (86.6％) were chronic inflammation.Cervical HPV load postoperatively negative rate was 85％ and the lower HPV load,the higher postoperatively negative rate.Conclusion LEEP is the effective treatment for cervical lesion and can significantly decrease the HPV-DNA loading at cervix.

Cardiovascular Diseases ; etiology ; prevention & control ; Humans ; Hypertension ; complications ; Risk Factors

OBJECTIVE:To prepare a Chinese medicinal agent for external application on burn wounds.METHODS:To establish the formula,preparation technic and quality control standard of burn oil solutions I and to observe the therapetuic effect.RESULTS:The preparation technic was feasible and method of quality control simple.The mean recovery was 99.68%(N=5),RSD=0.80%.Burn wounds

Objective To study the expression and clinical significance of proliferating cell nuclear antigen(PCNA) and PTEN in endometrial carcinoma.Methods The expression of PTEN and PCNA of 18 cases of proliferative endometrium(normal group),21 cases of atypical hyperplasia of endometrium(precancerous lesion group),and 22 cases of endometrial carcinoma(endometrial carcinoma group) was determined immunohistochemically(SP method).Results PCNA expression in the normal group(16.1%),precancerous lesion group(71.4%),and endometrial carcinoma group(95.5%) was significantly different(P