Child ; Clinical Laboratory Techniques ; Humans ; Tuberculosis ; diagnosis

Objective To investigate the self-management self-efficacy,health-related quality of life of hemodialysis patients and explore the relationship between them. Methods Self-management self-efficacy questionnaire and SF-36 were used,and 55 hemodialysis patients participated in this study. Pearson correlate and regression analysis were used to test the relationship between them. Results 55 questionnaires were available. Positively associated between self-management self-efficacy and HRQOL(r=0.268,P＜0.05);significantly positively associated between physical activity self-efficacy and HRQOL(r=0.409,P＜0.01). Regression analysis showed that physical activity self-efficacy is the factor of HRQOL (t=3.261,P＜0.01).Conclusion Physical activity self-efficacy is the factor of HRQOL in hemodialysis patients,increasing self-management self-efficacy is benefit to their HRQOL.

Gastrodin (GAS) is the major bioactive component of the extracts from the rhizome of Gastrodia elata Blume. The aim of this study is to investigate the transport of GAS in Caco-2 cells and the interaction of P-glycoprotein and GAS. The apparent permeability coefficients (Papp) of GAS were measured as a function of directions and concentrations. It was demonstrated that the efflux ratio was < 2.0 over the range of 50-500 micromol x L(-1) of GAS from bi-directional transport studies. The transport rate of GAS was dependent on the concentrations. Papp of GAS was not affected by transport directions, GAS concentration or the classical inhibitors of P-glycoprotein (verapamil and GF 120918). The cellular accumulation of GAS in Bcap37/MDR1 cells transected with hMDR1 gene, was similar to that in Bcap37 cells. The accumulation in both cell lines was concentration dependent. GAS did not affect the accumulation of Rhodamine 123 in Bcap37/MDR1 cells over the range of 50-500 micromol x L(-1). It indicated that the transport of GAS in Caco-2 cell monolayers mainly is by passive paracellular transport pathway. P-glycoprotein did not participate in the absorption of GAS in the intestine or the transport across the blood-brain barrier.

Objective To investigate the ability of theory of mind use in elders,and determine the role of inhibitory control in the use of theory of mind. Methods The participants included 30 young-old people ,30 oldold people and 30 young people. Their ability of theory of mind use and inhibitory control were examined with The Referential Communication Task and Hayling Sentence Completion Test,respectively. Results The correct rate of referential communication task was significantly lower in old-old people (0.43 ± 0.32) than those of young-old people (0.69 ±0.24, P=0. 001 ) and young people (0. 77 ±0. 25, P＜0. 001 ). Partial correlation analysis showed that the total errors in Hayling Test-B was negatively correlated with the correct rate of Referential Communication Task ( r= -0.442, P＜0.001 ). Hiberarchy regression analysis showed that the total errors in Hayling Test-B entered the equation ( β = -0.451, P＜0. 001 ) and could independently explained 16.8% of the variance (△R2 = 0. 168, △F = 19.359, P ＜ 0.001 ) after controlling for age. Conclusion Old-old people show worse performance in the use of theory of mind. The impairment of inhibitory control can partly explain the decline of theory of mind use in old-old people.

ObjectiveTo discuss the curative effects of sodium aescinate tablet on traumatic leg swelling.Methods168 cases were divided into four groups.54 cases of Ⅰ degree leg swelling were randomly divided into two groups,one group were treated with no drug and the other with sodium aescinate tablet.114 cases of Ⅱ and Ⅲ degree leg swelling were randomly divided into two groups,one group were treated with mannitol and the other with mannitol and sodium aescinate tablet.All cases were treated for 7 days and observed on leg swelling.ResultsThe curative effects of group with sodium aescinate tablet were superior to the group with no drug.The curative effects of group with both sodium aescinate tablet and mannitol are superior to group with mannitol.ConclusionSodium aescinate tablet had obviously curative effects on trautic leg swelling.Both sodium aescinate and mannitol could be used to treat leg swelling of Ⅱ and Ⅲ degree.

Objective To investigate the correlation of serum alpha-fetoprotein (AFP) with clinical pathological factors and prognosis of hepatocellular carcinoma ( HCC ).Methods Two hundred and twenty patients with HCC who hospitalized in our hospital from January 2007 to June 2009 were enrolled in this study.The relationship of AFP levels before operation to clinical pathological factors such as pathological grade,tumor size,tumor encapsulation,tumor numbers,portal vein tumor thrombus,TNM stage and postoperative recurrence were analyzed.Results Serum AFP concentration before operation were related with tumor size ( x2 =11.76,P ＜ 0.05 ),tumor encapsulation ( x2 =17.07,P ＜ 0.05 ),portal vein tumor thrombus ( x2 =8.944,P ＜ 0.05 ),higher TNM stage ( x2 =4.929,P ＜ 0.05 ) and early postoperative recurrence rate ( x2 =5.569,P ＜ 0.05 ).However,it had no correlation with pathological edmondson grade tumor numbers.Conclusion HCC patients with high serum AFP concentration often has greater tumor without encapsulation,high occurrence rate of portal vein tumor thrombus and higher TNM stage,they are more likely to early postoperative recurrence.Serum AFP before operation is useful not only for diagnosis,but also as a prognostic biomarker to predict prognosis and recurrence.

Objective To evaluate the activities of 18 pairs of antimicrobials combinations against non - duplicate clinical isolates of multidrug resistant Acinetobacter baumannii (MDRAB) in vitro.Methods Collect isolates of Acinetobacter baumannii from different patients from October 2009 to May 2010,which were isolated in Clinical Laboratory Center of Beijing Friendship Hospital,Capital Medical University.Use broth microdilution method to detect MIC of mono-antimicrobial,and checkerboard broth microdilution method to detect combinatied MIC,and calculate fractional inhibitory concentration (FIC) index to determine drug combinations effects.When the performance of the same drug combinations conflicted,appropriate strains were selected for screening of drug-resistant mechanisms by polymerase chain reaction( PCR),including efflux pump genes.Results In tests in vitro,rifampicin and polymyxin B,imipenem and gentamicin,cefepime and levofloxacin showed synergy at high proportion,68.1％,45.5％,40.9％,respectively.Minocycline and rifampicin,ampicillin/sulbactam and tobramycin.Ceftazidime and ciprofloxacin showed additive effect at high proportion,81.8％,68.2％,68.2％,respectively.There were several combinations which appeared the opposite effects to tested strains.Strains No.19 corresponding reaction was synergy and No.21,No.26 corresponding reactions were antagonism.The three strains above were selected for screening resistant mechanisms.The difference is that genotypes of adeS were negative in No.19 and positive in No.21 and No.26.Conclusion Rifampicin and polymyxin B combination showed synergy against the MDRAB in vitro,which can be considered as the treatment choice for critical infections caused by MDRAB.Imipenem and gentamicin,cefepime and levofloxacin also showed synergy in vitro,but in some isolates showed antagonism.This phenomenon may be due to the gene adeS activated by certain antibiotics,and the activated adeS drived efflux pump express or overexpress,which made the drugs in bacterial cells pumped out,causing antagonistic effect.The individual differences in strains should be considered when clinic strain apply these two combinations above.

The content of geniposide in Compound Zhizi Granules was determined With TLC-scanner. The optimum experiment design obtained with orthogonal test could eliminate the disturbance of other components. The result was satisfactory.

OBJECTIVE:To explore hospital pharmaceutical care mode to promote clinical rational drug use.METHODS:The consumption data of drugs for patients with cardiovascular and cerebrovascular diseases during 2006 in dispensary of traditional Chinese medicine of our hospital were investigated and analyzed.RESULTS:The ratio of drug combination increased with the rising of patients' age,so did the incidence of adverse drug reactions(ADR).CONCLUSION:Pharmacists' pharmaceutical care plays an important role in promoting patients' rational drug use and treatment.