Child ; Clinical Laboratory Techniques ; Humans ; Tuberculosis ; diagnosis

Background and purpose:Tumor necrosis factor related apoptosis-inducing ligand (TRAIL) is a death receptor ligand that can induce apoptosis in a variety of cancer cell lines, but not all cancer cells . Past studies suggested that some chemotherapeutic drugs intensified the sensitivity of TRAIL induced apoptosis.We investigated the effect and potential mechanism of combined paclitaxel and TRAIL on apoptosis of human gastric cancer cell in vitro. Methods:SGC7901 cells were cultured with paclitaxel at different concentrations(1,5,10,20,40 ?g/ml) and different time(12,24,36,48 hr), methyl thiazolyl tetrazolium (MTT) assay was used to measure the anticancer activity of paclitaxel.The ability of TRAIL alone,paclitaxel alone and TRAIL in combination with paclitaxel to induce apoptosis was measured by a flow cytometer. Expression of DR4,DR5, DcR1,DcR2,Caspase-8 and Bcl-2 mRNA was examined by RT-PCR.Results:The apoptosis rates of control group(C group),paclitaxel group(P group),TRAIL group (T group)and combination group(T+P)in 24 hr were 2.09%,10.65%,7.79% and 24.51%, respectively. The apoptosis rate in T+P group was significantly higher than that in C group, T group and P group(P

Objective:To develop an Online Game Cognitive Distortions Scale(OGCDS) and examine its applicability in Chinese adolescents.Methods:A pretest was given to 117 middle school students to determine a 17-item version of OGCDS.A sample of 495 middle school students were required to complete OGCDS,Internet Game Cognition Addiction Scale and Young's Internet Addiction Disorder scale.Reliability and validity of OGCDS were analyzed.Results:①The retest reliability,split-half and Cronbach's ? reliability coefficients of the total scale were 0.753,0.827 and 0.904,respectively.②Scores of the total scale and each subscale were both significantly correlated with scores of IGCAS and IAD scale at 0.01 levels (r=0.540～0.728;r=0.283～0.413).③Four factors were extracted by exploratory factor analysis,explaining 72.626% of the total variance;the load of each item varied from 0.490～0.886.④Confirmatory factor analysis confirmed that standard measurement model fit data well:?2=141.586,NFI=0.909,IFI=0.952,CFI=0.952,RMSEA=0.063.Conclusion:Reliability and validity of OGCDS is corresponded with psychometric standard.

Based on the cyanidine reaction, a quantitative electrocolorimetric method for the estimation of hesperidin is described. The main steps of the procedure are as follows:Extraction: The sample is washed successively with 95% alcohol and ether until the washings are free from yellow color when alkali is added. The washed sample is then extracted under re fluxing for 90 minutes with 50% alcohol (the best ratio between the sample and alcohol ia 1:100 by weight), filtered while hot, and the filtrates is kept at 70℃ until used.Color producing and comparison: Five ml of the above extract are added into a test tube containing 250 mg of magnesium powder. The tube thus prepared is placed into warm water of 15℃ and 2 ml of cone. HCl are added slowly (0.2 ml for every 2 minutes) with gentle shaking. After the addition of HC1 is completed, the content of the tube is shaken and incubated at 37℃ for 1 hour. The extract is then cooled, filtered and its optical density is determined. The error introduced by the colored sample solution is corrected by setting the optical density reading of the blank at zero before the reading of the unknown. The blank was prepared similarly as the unknown except that the reaction of magnesium powder with added HCl was completed before the addition of sample solution.The hesperidin content of the sample is determined by interpolation of the optical density values on a standard curve prepared by a series of gradient concentration of hesperidin.An amount of 0.025 mg hesperidin in 5 ml solution can be determined quantitatively by this method and the recovery was 97.1-100.3%.

117 guinea pigs were divided into groups, receiving 1mg, 5mg, 50mg and 100mg Vit C per os daily respectively in 2-4 weeks. There were conducted four experiments: (1) the effect of different vitamin C levels on the cold tolerance and adaptation in guinea pigs; (2) effect of large dose of vitemin C on sudden and long time exposure to cold: (3) the action of vitamin C, in large dose and short time in combination with hormones, on cold tolerance of guinea pigs; (4) effect of large dose of vitamin C on the myocardial enzyme activity, during the process of cold adaptation. Rectal temperature, body weight, weight and vitamin C contents of adrenal glands, the excretion of urinary 17-Ketostero'ids, activity of myocardial succinic dehydrogenase and cytochrom C oxidase were observed. The results revealed that large dose of Vitamin C per os might lessen the lowering of rectal temperature and body weight after exposure to cold; promote the activities of myocardial enzymes. Better results were obtained with combination of vitamin C with cortisones. It was noted that large dose of vitamin C apparently increased and accelerated the tolerance and adaptation of organisms to cold.

Objective To compare curative effects between laparoscopic oviduct-sparing operation and medical interventional treatment for the management of tubal pregnancy.Methods A total of 63 patients with tubal pregnancy were randomly divided into two groups.One group underwent laparoscopic salpingotomy and aspiration of the gestational sac content, while the other group underwent intra-tubal injection of methotrexate(MTX).Curative effects of the two procedures were compared.Results Laparoscopic oviduct-sparing operation was successfully performed on one session in 31 out of 32 patients,the success rate being 96.9%.Medical interventional treatment was conducted successfully in 24 out of 31 patients,23 of whom received one dose of MTX and 1 of whom received two doses,the success rate being 77.4%.There was no statistical difference between the two groups in success rates (?~2=3.765,P=0.052).The time for serum ?-hCG to normal levels was significantly shorter in patients receiving laparoscopic oviduct-sparing operation(9.1?2.1 d) than in patients receiving interventional treatment(26.3?3.9 d)(t=-20.974,P=0.000).Conclusions Both laparoscopic oviduct-sparing operation and medical interventional treatment can effectively preserve affected oviduct for patients with tubal pregnancy.As compared with medical interventional treatment,laparoscopic oviduct-sparing operation has a broader range of indications,less side effects,and a shorter recovery time of serum ?-hCG.

Objective To study the application of ultrasonic freestyle extended imaging(FEI) in thyroid,breast,artery,vein diseases.Methods Thirty-five patients with above mentioned diseases were examined by FEI and the images were analyzed.Results FEI could entirely show the scan region needed.It also could enhance image quality,show the physiologic radian of the body,measure the nidi accurately.Conclusion The FEI technology provides more image information and is of great help to improve the clinic diagnosis ratios.

Primary biliary cirrhosis(PBC) is a chronic,progressive cholestatic liver disease of unknown cause that usually affects middle-aged women.Ursodeoxycholic acid(UDCA)is the first selected drug to PBC which has definite effect.Treament of PBC with UDCA combined with immunodepressant become presents research focuses.Liver transplantation is the only treatment of the final stage of PBC.

There were 17 literatures related to the clinical applications of natural killer cells in hematological diseases in the 18th annual meeting of the European Hematology Association (EHA).Here an overview of these literatures is given that helps to tnderstand how natural killer cells can be explored for future clinical interventions.

OBJECTIVE:To explore hospital pharmaceutical care mode to promote clinical rational drug use.METHODS:The consumption data of drugs for patients with cardiovascular and cerebrovascular diseases during 2006 in dispensary of traditional Chinese medicine of our hospital were investigated and analyzed.RESULTS:The ratio of drug combination increased with the rising of patients' age,so did the incidence of adverse drug reactions(ADR).CONCLUSION:Pharmacists' pharmaceutical care plays an important role in promoting patients' rational drug use and treatment.